Transcriptional activity of TGFβ1 and its receptors genes in thyroid gland

Kajdaniuk, Dariusz; Marek, Anna; Marek, Bogdan; Mazurek, Urszula; Fila-Daniłow, Anna; Fołtyn, Wanda; Morawiec-Szymonik, Elżbieta; Siemińśka, Lucyna; Nowak, Mariusz; Głogowska-Szeląg, Joanna; Niedziołka-Zielonka, Danuta; Seemann, Michał; Kos–Kudła, Beata

doi:10.5603/ep.a2016.0045

Cited by 4 publications

(3 citation statements)

References 21 publications

(40 reference statements)

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“…The authors observed an elevated mRNA expression of TGFB1 in PTC (n=06) compared to multinodular goiter (n=22, p=0.015) and Graves' disease (n=08, p=0.001). In this same study, they performed a serum analysis of TGF-β1 that did not present differences (32), supporting the similar findings of Zivancevic-Simonovic and cols. (33) and suggesting a local pathological effect of the protein.…”

Section: Discussionsupporting

confidence: 83%

Clinical utility of TGFB1 and its receptors (TGFBR1 and TGFBR2) in thyroid nodules: evaluation based on single nucleotide polymorphisms and mRNA analysis

Peres¹,

Teodoro²,

Amaral³

et al. 2021

Archives of Endocrinology and Metabolism

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Objective: Abnormalities involving the TGFB1 gene and its receptors are common in several types of cancer and often related to tumor progression. We investigated the role of single nucleotide polymorphisms (SNP) in the susceptibility to cancer, their impact on its features, as well as the role of mRNA expression of these genes in thyroid malignancy. Materials and methods: We genotyped TGFB1, TGFBR1, and TGFBR2 SNPs in 157 papillary thyroid cancer (PTC) patients and 200 healthy controls. Further, we investigated RNA samples of 47 PTC and 80 benign nodules, searching for differential mRNA expression. Results: SNPs rs1800472 and rs1800469 were associated with characteristics of PTC aggressiveness. Effect predictor software analysis of nonsynonymous SNP rs1800472 indicated increasing protein stability and post-translational changes. TGFB1 mRNA expression was upregulated in PTC and downregulated in benign samples, differentiating malignant from benign nodules (p<0.0001); PTC from goiter (p<0.0001); and PTC from FA (p<0.0001). TGFBR1 mRNA expression was upregulated in goiter and PTC, but downregulated in FA, distinguishing PTC from goiter (p=0.0049); PTC from FA (p<0.0001); and goiter from FA (p=0.0267). On the other hand, TGFBR2 was downregulated in all histological types analyzed and was not able to differentiate thyroid nodules. Conclusion: TGFB1 polymorphism rs1800472 may confer greater activity to TGF-β1 in the tumor microenvironment, favoring PTC aggressiveness. Evaluation of TGFB1 and TGFBR1 mRNA levels may be useful to identify malignancy in thyroid nodules.

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Section: Discussionsupporting

confidence: 83%

Clinical utility of TGFB1 and its receptors (TGFBR1 and TGFBR2) in thyroid nodules: evaluation based on single nucleotide polymorphisms and mRNA analysis

Peres¹,

Teodoro²,

Amaral³

et al. 2021

Archives of Endocrinology and Metabolism

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“…However, there is evidence of a more complex reciprocal regulation for both pathways [49]. Thus, the increased BMPR2 expression we found in our study could be paired with a decrease in the expression of the TGF-b1, something that has been demonstrated for patients with GD in a recent study carried out in biopsy samples from thyroid pathology patients [50].…”

Section: Discussionsupporting

confidence: 74%

Functional assessment of the BMPR2 gene in lymphoblastoid cell lines from Graves’ disease patients

Pousada

Lago-Docampo

Prado

et al. 2017

J Cellular Molecular Medi

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In this study, we analysed the possible influence of the c.419‐43delT BMPR2 variant in patients with Graves’ disease (GD), in a molecular basis, focusing our efforts on possible alterations in the mRNA processing and synthesis. The molecular assessment of this variant in patients with GD would shed light on the association between the BMPR2 gene and the disease. The variant was detected in 18%, 55% and 10% of patients with pulmonary arterial hypertension, GD and in general population, respectively. Patients with GD fold change showed increased BMPR2 expression when matched against the controls, with a mean of 4.21 ± 1.73 (P = 0.001); BMPR2 was overexpressed in the analysed cell cycle stages. Fold change analysis of variant carriers and non‐carriers showed slight overexpression and differences between phases, but none of them were statistically significant. BMPR2 expression was confirmed in the lymphoblastoid cell lines (LCLs) with a molecular weight of 115 kD, and no differences between variant carriers and non‐carriers were detected. To conclude, the BMPR2 variant c.419‐19delT appears in high frequency in patients with GD, and independently of its presence, BMPR2 is overexpressed in the LCLs from the GD patients tested. This increase could be paired with the described decreased expression of transforming growth factor‐β1 in thyroid tissue from patients with GD.

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“…Thus, it cannot be excluded that processes resulting from autoimmunity in the thyroid gland, leading to parenchymal destruction and fibrosis, also occur in myocardial tissue. Indeed, in both autoimmune thyroiditis and HF in diabetes, there is increased transforming growth factor-b expression associated with increased fibrotic processes [32,33]. Our results showed a significant positive correlation between the presence of connective tissue echoes in thyroid on ultrasound and echocardiographic parameters reflecting systolic and diastolic function of LV.…”

Section: Original Papersupporting

confidence: 48%

Association of antithyroid peroxidase antibodies with cardiac function in euthyroid women with type 1 diabetes mellitus — assessment with two-dimensional speckle‑tracking echocardiography

et al. 2022

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Introduction:The presence of diabetes is associated with loss of cardioprotection in premenopausal women; however, the mechanisms involved remain unknown. Autoimmune factors are suspected to play a role in cardiovascular complications, especially in type 1 diabetes (T1DM). The aim of this pilot study was to explore whether antithyroid peroxidase antibody (aTPO) as a marker of increased immune activity is related to cardiac dysfunction in young, asymptomatic women with T1DM. Material and methods: Eighty-eight euthyroid women (59 with T1DM and 29 healthy controls) underwent physical examination, laboratory tests, thyroid ultrasound, and two-dimensional speckle-tracking echocardiography. According to the antiperoxidase antibodies (aTPO) titre, the T1DM women were divided into an aTPO positive (T1DM aTPO+) (n = 34) and an aTPO negative (T1DM aTPO-) (n = 25) group. The relationship between thyroid autoimmunity parameters and echocardiographic parameters was evaluated. Results: Global longitudinal strain (GLS) was slightly reduced in the T1DM aTPO+ group compared to T1DM aTPO-and significantly compared to controls (p = 0.051 and p = 0.015, respectively). Although, the lower values of longitudinal strain of left ventricular were found in the majority of segments in the T1DM aTPO+ group in comparison to T1DM aTPO-and controls, significant differences were only found in the two-chamber view (specifically in the anterior segments) between the T1DM aTPO+ and T1DM aTPO-groups (p = 0.030) and in the four-chamber view (specifically in the anterolateral segments) between the T1DM aTPO+ group and controls (p = 0.021). Echocardiographic parameters of diastolic and systolic function of both ventricles were significantly correlated with parameters of thyroid autoimmunity. A logistic regression analysis showed that Hashimoto's thyroiditis (HT) duration [odds ratio (OR): 0.997, 95% confidence interval (CI): 0.995-0.999, p = 0.008), the dose of levothyroxine (OR: 0.814, 95% CI: 0.689-0.960, p = 0.013), and reduced echogenicity on thyroid ultrasound (OR: 0.309, 95% CI: 0.120-0.793, p = 0.013) had a significant influence on reduced GLS. Conclusions: Our results suggest that coexistence of aTPO with T1DM was associated with poorer myocardial function, particularly in the anterior and anterolateral segments, which may be related to an autoimmune factor. The impaired function of these segments is probably the first sign of myocardial systolic dysfunction in women with T1DM, which needs to be confirmed in further studies.

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Transcriptional activity of TGFβ1 and its receptors genes in thyroid gland

Cited by 4 publications

References 21 publications

Clinical utility of TGFB1 and its receptors (TGFBR1 and TGFBR2) in thyroid nodules: evaluation based on single nucleotide polymorphisms and mRNA analysis

Clinical utility of TGFB1 and its receptors (TGFBR1 and TGFBR2) in thyroid nodules: evaluation based on single nucleotide polymorphisms and mRNA analysis

Functional assessment of the BMPR2 gene in lymphoblastoid cell lines from Graves’ disease patients

Association of antithyroid peroxidase antibodies with cardiac function in euthyroid women with type 1 diabetes mellitus — assessment with two-dimensional speckle‑tracking echocardiography

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