Transcriptional activity of Pannier is regulated negatively by heterodimerization of the GATA DNA-binding domain with a cofactor encoded by the <i>u-shaped</i> gene of <i>Drosophila</i>

Haenlin, Marc; Cubadda, Yolande; Blondeau, F; Heitzler, Pascal; Lutz, Yves; Simpson, Patricia; Ramain, Philippe

doi:10.1101/gad.11.22.3096

Cited by 184 publications

(178 citation statements)

References 54 publications

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“…In addition, recent studies have suggested that FOG-1 can interact with either GATA-1 or GATA-2 to promote megakaryopoiesis, arguing against the previous notion that FOG-1 functions independently in this process (5). Furthermore, in Drosophila, Ush repression of heart and sensory bristle development apparently requires the GATA binding partner Pannier (6,37). Unequivocal examples of FOG-independent functions have not been found.…”

Section: Discussionmentioning

confidence: 71%

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Combinatorial interactions of Serpent, Lozenge, and U-shaped regulate crystal cell lineage commitment during Drosophila hematopoiesis

Fossett

Hyman

Gajewski

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

124

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The GATA factor Serpent (Srp) is required for hemocyte precursor formation during Drosophila hematopoiesis. These blood cell progenitors give rise to two distinct lineages within the developing embryo. Lozenge, a Runx protein homologue, and Glial cells missing-1 and -2 are essential for crystal cell and plasmatocyte production, respectively. In contrast U-shaped, a Friend of GATA class factor, antagonizes crystal cell formation. Here we show that Srp, Lozenge, and U-shaped interact in different combinations to regulate crystal cell lineage commitment. Coexpression of Srp and Lozenge synergistically activated the crystal cell program in both embryonic and larval stages. Furthermore, expression of Lozenge and SrpNC, a Srp isoform with N-and C-terminal zinc fingers, inhibited u-shaped expression, indicating that crystal cell activation coincided with the down-regulation of this repressor-encoding gene. In contrast, whereas U-shaped and SrpNC together blocked crystal cell production, coexpression of U-shaped with noninteracting Srp proteins failed to prevent overproduction of this hemocyte population. Such results indicated that U-shaped and SrpNC must interact to block crystal cell production. Taken together, these studies show that the specialized SrpNC isoform plays a pivotal role during crystal cell lineage commitment, acting as an activator or repressor depending on the availability of specific transcriptional coregulators. These findings provide definitive proof of the combinatorial regulation of hematopoiesis in Drosophila and an in vivo demonstration of GATA and Runx functional interaction in a blood cell commitment program.

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Section: Discussionmentioning

confidence: 71%

“…In general, FOG proteins bind the N-terminal zinc-finger of their respective GATA partners. This interaction modifies GATAregulated transcription in a variety of tissues and in taxa ranging from flies to humans (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). Hematopoietic coregulators of GATA factors may include members of the Runx family.…”

mentioning

confidence: 99%

Combinatorial interactions of Serpent, Lozenge, and U-shaped regulate crystal cell lineage commitment during Drosophila hematopoiesis

Fossett

Hyman

Gajewski

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

124

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“…We therefore sought to estimate the affinity of the 15 N-labeled USH-F9⅐Pnr-NF interaction by analyzing our 1 H, 15 N HSQC titration data. Several USH-F9 resonances (Cys-14, Val-21, and Tyr-31) were in fast exchange, and independent fitting of their chemical shift changes using a simple heterodimerization model (Equation 5 and Fig. 3A) gave an average K a of (1.3 Ϯ 0.6) ϫ 10 4 M Ϫ1 .…”

Section: Resultsmentioning

confidence: 99%

“…The peak position for a resonance in intermediate exchange does not accurately reflect the weighted average between the free and bound states of the protein and can no longer be determined by a simple expression such as Equation 5. However, the amplitude of a signal from USH-F9 as a function of frequency for a particular Pnr-NF concentration can be determined from the imaginary component of the complex quantity G() (34), which is given by…”

Section: Methodsmentioning

confidence: 99%

Characterization of the Conserved Interaction between GATA and FOG Family Proteins

Kowalski¹,

Liew²,

Matthews³

et al. 2002

Journal of Biological Chemistry

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The N-terminal zinc finger (ZnF) from GATA transcription factors mediates interactions with FOG family proteins. In FOG proteins, the interacting domains are also ZnFs; these domains are related to classical CCHH fingers but have an His 3 Cys substitution at the final zinc-ligating position. Here we demonstrate that different CCHC fingers in the FOG family protein U-shaped contact the N-terminal ZnF of GATA-1 in the same fashion although with different affinities. We also show that these interactions are of moderate affinity, which is interesting given the presumed low concentrations of these proteins in the nucleus. Furthermore, we demonstrate that the variant CCHC topology enhances binding affinity, although the His 3 Cys change is not essential for the formation of a stably folded domain. To ascertain the structural basis for the contribution of the CCHC arrangement, we have determined the structure of a CCHH mutant of finger nine from U-shaped. The structure is very similar overall to the wild-type domain, with subtle differences at the C terminus that result in loss of the interaction in vivo. Taken together, these results suggest that the CCHC zinc binding topology is required for the integrity of GATA-FOG interactions and that weak interactions can play important roles in vivo.

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“…, an amorph allele of emc (Brown et al 1995); Bar 1 (B 1 )/FM7, a dominant gain-offunction mutation generated by duplication of the 16A region containing BarH1 and BarH2 genes, referred to as Bar (B); Df(1)Bar , a deficiency in which both B genes are deleted (Higashijima et al 1992); sgg 1 /FM7a Dp(1;2;4) w 1 , a lethal recessive X-ray allele of sgg (Bourouis et al 1990); Df (2L) BSC7/CyO, UAS-pnr D4 (Haenlin et al 1997); y w; FRT82, pnr vx6 / TM6B (Heitzler et al 1996); UAS-pnr ENR , a dominant-negative form of pnr, which contains the repressor domain from Engrailed (amino acids 2-298) (Klinedinst and Bodmer 2003); UAS-ush (Fossett et al 2001); Df (3L) Iro-C DFM3 FRT80/TM6B, a deficiency for ara, caup, and promoter for mirr, UAS-ara (Gomez-Skarmeta et al 1996); UAS-mirr (McNeill et al 1997); y w ey-FLP (Newsome et al 2000); and pnr-GAL4 . All stocks are described in FlyBase (http:/ /flybase.bio.indiana.edu).…”

mentioning

confidence: 99%

Genetic Interaction of Lobe With Its Modifiers in Dorsoventral Patterning and Growth of the Drosophila Eye

et al. 2005

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Dorsoventral (DV) patterning is essential for growth of the Drosophila eye. Recent studies suggest that ventral is the default state of the early eye, which depends on Lobe (L) function, and that the dorsal fate is established later by the expression of the dorsal selector gene pannier (pnr). However, the mechanisms of regulatory interactions between L and dorsal genes are not well understood. For studying the mechanisms of DV patterning in the early eye disc, we performed a dominant modifier screen to identify additional genes that interact with L. The criterion of the dominant interaction was either enhancement or suppression of the L ventral eye loss phenotype. We identified 48 modifiers that correspond to 16 genes, which include fringe ( fng), a gene involved in ventral eye patterning, and members of both Hedgehog (Hh) and Decapentaplegic (Dpp) signaling pathways, which promote L function in the ventral eye. Interestingly, 29% of the modifiers (6 enhancers and 9 suppressors) identified either are known to interact genetically with pnr or are members of the Wingless (Wg) pathway, which acts downstream from pnr. The detailed analysis of genetic interactions revealed that pnr and L mutually antagonize each other during second instar of larval development to restrict their functional domains in the eye. This time window coincides with the emergence of pnr expression in the eye. Our results suggest that L function is regulated by multiple signaling pathways and that the mutual antagonism between L and dorsal genes is crucial for balanced eye growth.

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Transcriptional activity of Pannier is regulated negatively by heterodimerization of the GATA DNA-binding domain with a cofactor encoded by the u-shaped gene of Drosophila

Cited by 184 publications

References 54 publications

Combinatorial interactions of Serpent, Lozenge, and U-shaped regulate crystal cell lineage commitment during Drosophila hematopoiesis

Combinatorial interactions of Serpent, Lozenge, and U-shaped regulate crystal cell lineage commitment during Drosophila hematopoiesis

Characterization of the Conserved Interaction between GATA and FOG Family Proteins

Genetic Interaction of Lobe With Its Modifiers in Dorsoventral Patterning and Growth of the Drosophila Eye

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