Transcriptional Activity of Nuclear Factor Kappa B (NF-κB) in Post-Traumatic Sensory Neurons (a histochemical study)

Гущина, С. В.; Волкова, О. В.; Kruglyakov, P. P.; Magoulas, Charalambos

doi:10.1007/s11055-011-9404-y

Cited by 1 publication

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References 13 publications

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“…It was noteworthy that CBS enzyme upregulation was correlated with the increase in the amount of NF-κB (p65) in the cell nucleus. NF-κB, a pivotal player in inflammatory responses and neuropathy, is constitutively expressed in the primary sensory neurons and glial cells in dorsal root ganglion [41]. It is known that NF-κB participates in pain processes, an effect attributed to the effect of NF-κB on gene transcription.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of Cystathionine Beta-Synthetase Expression by Nuclear Factor-Kappa B Activation Contributes to Visceral Hypersensitivity in Adult Rats with Neonatal Maternal Deprivation

Xie

et al. 2012

Mol Pain

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BackgroundIrritable bowel syndrome (IBS) is characterized by chronic visceral hyperalgesia (CVH) that manifested with persistent or recurrent abdominal pain and altered bowel movement. However, the pathogenesis of the CVH remains unknown. The aim of this study was to investigate roles of endogenous hydrogen sulfide (H2S) producing enzyme cystathionine beta-synthetase (CBS) and p65 nuclear factor-kappa B subunits in CVH.ResultsCVH was induced by neonatal maternal deprivation (NMD) in male rats on postnatal days 2–15 and behavioral experiments were conducted at the age of 7–15 weeks. NMD significantly increased expression of CBS in colon-innervating DRGs from the 7th to 12th week. This change in CBS express is well correlated with the time course of enhanced visceromoter responses to colorectal distention (CRD), an indicator of visceral pain. Administration of AOAA, an inhibitor of CBS, produced a dose-dependent antinociceptive effect on NMD rats while it had no effect on age-matched healthy control rats. AOAA also reversed the enhanced neuronal excitability seen in colon-innervating DRGs. Application of NaHS, a donor of H2S, increased excitability of colon-innervating DRG neurons acutely dissociated from healthy control rats. Intrathecal injection of NaHS produced an acute visceral hyperalgesia. In addition, the content of p65 in nucleus was remarkably higher in NMD rats than that in age-matched controls. Intrathecal administration of PDTC, an inhibitor of p65, markedly reduced expression of CBS and attenuated nociceptive responses to CRD.ConclusionThe present results suggested that upregulation of CBS expression, which is mediated by activation of p65, contributes to NMD-induced CVH. This pathway might be a potential target for relieving CVH in patients with IBS.

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Section: Discussionmentioning

confidence: 99%

Upregulation of Cystathionine Beta-Synthetase Expression by Nuclear Factor-Kappa B Activation Contributes to Visceral Hypersensitivity in Adult Rats with Neonatal Maternal Deprivation

Xie

et al. 2012

Mol Pain

View full text Add to dashboard Cite

show abstract

Transcriptional Activity of Nuclear Factor Kappa B (NF-κB) in Post-Traumatic Sensory Neurons (a histochemical study)

Cited by 1 publication

References 13 publications

Upregulation of Cystathionine Beta-Synthetase Expression by Nuclear Factor-Kappa B Activation Contributes to Visceral Hypersensitivity in Adult Rats with Neonatal Maternal Deprivation

Upregulation of Cystathionine Beta-Synthetase Expression by Nuclear Factor-Kappa B Activation Contributes to Visceral Hypersensitivity in Adult Rats with Neonatal Maternal Deprivation

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