Transcriptional activators Cat8 and Sip4 discriminate between sequence variants of the carbon source-responsive promoter element in the yeast Saccharomyces cerevisiae

Roth, Stephanie; Kumme, Jacqueline; Schüller, Hans‐Joachim

doi:10.1007/s00294-003-0476-2

Cited by 52 publications

(47 citation statements)

References 37 publications

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“…It is possible that these regulators recognize subsets of CSREs with different affinities, allowing regulation of both common and distinct genes. This hypothesis is consistent with the fact that mutant CSREs show differential activation by Cat8 and Sip4 (42). Rds2 and Cat8 have a limited set of common target genes (see below).…”

Section: Discussionsupporting

confidence: 70%

Regulation of Gluconeogenesis in Saccharomyces cerevisiae Is Mediated by Activator and Repressor Functions of Rds2

Soontorngun¹,

Larochelle²,

Drouin

et al. 2007

Molecular and Cellular Biology

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In Saccharomyces cerevisiae, RDS2 encodes a zinc cluster transcription factor with unknown function. Here, we unravel a key function of Rds2 in gluconeogenesis using chromatin immunoprecipitation-chip technology. While we observed that Rds2 binds to only a few promoters in glucose-containing medium, it binds many additional genes when the medium is shifted to ethanol, a nonfermentable carbon source. Interestingly, many of these genes are involved in gluconeogenesis, the tricarboxylic acid cycle, and the glyoxylate cycle. Importantly, we show that Rds2 has a dual function: it directly activates the expression of gluconeogenic structural genes while it represses the expression of negative regulators of this pathway. We also show that the purified DNA binding domain of Rds2 binds in vitro to carbon source response elements found in the promoters of target genes. Finally, we show that upon a shift to ethanol, Rds2 activation is correlated with its hyperphosphorylation by the Snf1 kinase. In summary, we have characterized Rds2 as a novel major regulator of gluconeogenesis.

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Section: Discussionsupporting

confidence: 70%

Regulation of Gluconeogenesis in Saccharomyces cerevisiae Is Mediated by Activator and Repressor Functions of Rds2

Soontorngun¹,

Larochelle²,

Drouin

et al. 2007

Molecular and Cellular Biology

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“…Two of these factors, Cat8 and Sip4, both contain zinc finger DNA binding domains similar to that in Gal4 (a C6 zinc cluster) (145,206) and bind specifically to a set of carbon source responsive elements (CSRE) under derepressing conditions (300, 395). Cat8 and Sip4 are closely related structurally and appear to have slightly different affinities for variants of the CSRE sequence (319). Although Sip4-dependent transcriptional activation has been shown to require phosphorylation of Sip4 by Snf1 kinase, removal of Sip4 has little or no effect on CSRE-regulated genes (141,145,206,299).…”

Section: Snf1-mediated Signal Transduction In Glucose Repression and mentioning

confidence: 98%

Glucose Signaling in Saccharomyces cerevisiae

Santangelo

2006

Microbiol Mol Biol Rev

479

481

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SUMMARY Eukaryotic cells possess an exquisitely interwoven and fine-tuned series of signal transduction mechanisms with which to sense and respond to the ubiquitous fermentable carbon source glucose. The budding yeast Saccharomyces cerevisiae has proven to be a fertile model system with which to identify glucose signaling factors, determine the relevant functional and physical interrelationships, and characterize the corresponding metabolic, transcriptomic, and proteomic readouts. The early events in glucose signaling appear to require both extracellular sensing by transmembrane proteins and intracellular sensing by G proteins. Intermediate steps involve cAMP-dependent stimulation of protein kinase A (PKA) as well as one or more redundant PKA-independent pathways. The final steps are mediated by a relatively small collection of transcriptional regulators that collaborate closely to maximize the cellular rates of energy generation and growth. Understanding the nuclear events in this process may necessitate the further elaboration of a new model for eukaryotic gene regulation, called “reverse recruitment.” An essential feature of this idea is that fine-structure mapping of nuclear architecture will be required to understand the reception of regulatory signals that emanate from the plasma membrane and cytoplasm. Completion of this task should result in a much improved understanding of eukaryotic growth, differentiation, and carcinogenesis.

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“…Cat8-binding sequences were compared to the consensus determined in reference 38, revealing multiple mismatches in the CSREs at ADH2 and ATO3, where Adr1 affects Cat8 binding (Table 6). Some of these mismatches severely affect in vitro binding of Cat8 (38). ADH2 has an additional upstream CSRE with only two mismatches, but we do not know if it is bound by Cat8.…”

Section: Relative Position and Binding Site Sequence May Determine Ifmentioning

confidence: 99%

Combined Global Localization Analysis and Transcriptome Data Identify Genes That Are Directly Coregulated by Adr1 and Cat8

Tachibana

Yoo

Tagne

et al. 2005

Molecular and Cellular Biology

141

164

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In Saccharomyces cerevisiae, glucose depletion causes a profound alteration in metabolism, mediated in part by global transcriptional changes. Many of the transcription factors that regulate these changes act combinatorially. We have analyzed combinatorial regulation by Adr1 and Cat8, two transcription factors that act during glucose depletion, by combining genome-wide expression and genome-wide binding data. We identified 32 genes that are directly activated by Adr1, 28 genes that are directly activated by Cat8, and 14 genes that are directly regulated by both. Our analysis also uncovered promoters that Adr1 binds but does not regulate and promoters that are indirectly regulated by Cat8, stressing the advantage of combining global expression and global localization analysis to find directly regulated targets. At most of the coregulated promoters, the in vivo binding of one factor is independent of the other, but Adr1 is required for optimal Cat8 binding at two promoters with a poor match to the Cat8 binding consensus. In addition, Cat8 is required for Adr1 binding at promoters where Adr1 is not required for transcription. These data provide a comprehensive analysis of the direct, indirect, and combinatorial requirements for these two global transcription factors.Cells respond to stresses such as variations in temperature, pH or osmotic or nutrient conditions by altering the expression of genes that allow the cell to respond to the new environment (10). These transcriptional changes are initiated by signals that converge on a limited number of transcription factors, which then act combinatorially to control many genes in multiple pathways. Microarrays can be used to identify these coordinated, combinatorial responses by detecting both the localization of key transcription factors and the corresponding changes in the transcriptome (1).

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Transcriptional activators Cat8 and Sip4 discriminate between sequence variants of the carbon source-responsive promoter element in the yeast Saccharomyces cerevisiae

Cited by 52 publications

References 37 publications

Regulation of Gluconeogenesis in Saccharomyces cerevisiae Is Mediated by Activator and Repressor Functions of Rds2

Regulation of Gluconeogenesis in Saccharomyces cerevisiae Is Mediated by Activator and Repressor Functions of Rds2

Glucose Signaling in Saccharomyces cerevisiae

Combined Global Localization Analysis and Transcriptome Data Identify Genes That Are Directly Coregulated by Adr1 and Cat8

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