Transcriptional activation of cyclooxygenase-2 by tumor suppressor p53 requires nuclear factor-kappaB

Benoît, Valérie; Moraes, Elaine Lazzaroni; Dar, Nazir Ahmad; Taranchon, E.; Bours, Vincent; Hautefeuille, A.; Tanière, Philippe; Chariot, Alain; Scoazec, Jean‐Yves; Gallo, C.; Merville, Marie‐Paule; Hainaut, Pierre

doi:10.1038/sj.onc.1209579

Cited by 53 publications

(49 citation statements)

References 50 publications

(51 reference statements)

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“…Thus, this p53-dependent SGK induction can be seen as compensatory mechanism, as previously reported for other p53-dependent genes coding for pro-survival candidates such as COX-2, for example [46,47]. This negative feedback loop may also explain why the Elongator deficient cells are not significantly more sensitive to the DNA-damaging-mediated apoptotic pathway.…”

Section: Discussionsupporting

confidence: 62%

Deregulated expression of pro-survival and pro-apoptotic p53-dependent genes upon Elongator deficiency in colon cancer cells

Cornez

Creppe

Gillard

et al. 2008

Biochemical Pharmacology

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b i o c h e m i c a l p h a r m a c o l o g y 7 5 ( 2 0 0 8 ) 2 1 2 2 -2 1 3Abbreviations: B2M, beta-2-microglobulin; BARD1, BRCA1-associated RING domain 1; BAX, BCL2-associated X protein; BTG2, B-cell translocation gene 2; CDK9, cyclin-dependent kinase 9; DMEM, Dulbecco's modified Eagle's medium; DUSP4, dual-specificity phosphatase 4; Elp, Elongator protein; EMEM, Eagle's modified essential medium; FACT, facilitates chromatin transcription; GFP, green fluorescent protein; HA, haemagglutinin; hELP1, human ELP1; IKAP, IKK-associated protein; IKK, inhibitor of kappa light chain gene enhancer in B cells kinase complex; NR2F2, nuclear receptor subfamily 2, group F, member 2; p21, cyclin-dependent kinase inhibitor 1A; PARP, poly (ADP-ribose) polymerase 1; PKIB, protein kinase, cAMP-dependent catalytic, inhibitor beta; RhoE/Rnd3, Rho family GTPase 3; SESN2, sestrin 2; SGK, serum-and glucocorticoid-induced protein kinase; shRNA, short hairpin RNA; TFIIF, general transcription factor IIF; TFIIH, general transcription factor IIH; TNFRSF10D, tumor necrosis factor receptor superfamily, member 10D.

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Section: Discussionsupporting

confidence: 62%

Deregulated expression of pro-survival and pro-apoptotic p53-dependent genes upon Elongator deficiency in colon cancer cells

Cornez

Creppe

Gillard

et al. 2008

Biochemical Pharmacology

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“…Many findings have contributed to establishing the relationship between the expression of apoptosis and COX-2 (Benoit et al 2006;de Moraes et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies on A549 cells have indicated that Akt and p38kinase pathways mediate apoptosis and inflammation (Jang 2009;Boo et al 2011). These pathways also play an important role in the regulation of many cellular responses, such as cell differentiation, proliferation, and cell growth.…”

Section: Introductionmentioning

confidence: 99%

Gallotannin regulates apoptosis and COX-2 expression via Akt and p38kinase pathway in human lung cancer cell line, A549

Gweon

Chung

et al. 2012

Animal Cells and Systems

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Gallotannin (GT) is derived from plant poly phenol and is associated with biological actions in a wide range of cells. In this study, we evaluated the effect of GT on apoptosis and cyclooxygenase-2 (COX-2) expression and attempted to shed light on the mechanism of action in A549 human lung carcinoma cells. We found that GT dramatically induced apoptosis as demonstrated by expression of p53 and active caspase-3 via western blot analysis and fragmented DNA as detected by DNA fragmentation and DAPI staining. We also observed that GT significantly causes COX-2 expression in a dose-dependent manner determined by western blot analysis. Phosphorylation of Akt and p38 was considerably increased by GT in A549 human lung carcinoma cells. Inhibition of Akt and p38kinase with LY294002 or SB203580 suppressed GT-induced apoptosis and COX-2 expression. Furthermore, we have shown that prevention of COX-2 with NS398 or indomethacin does not any effects on apoptosis induced by GT. Taken together, our present results suggest that GT regulates apoptosis and COX-2 expression through Akt and p38kinase pathway in A549, human lung carcinoma cells.

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“…Finally, either dependent on the HB-EGF-induced activation of the ras/raf/MAPK pathway or in cooperation with NF-kB, p53 induces expression of the cyclooxygenase 2 (Cox-2) gene following exposure to various DNA-damaging agents. 44,147 Cox-2, a crucial enzyme in prostaglandin synthesis is often overexpressed in many solid tumors and associated with tumor aggressiveness due to its well documented anti-apoptotic and tumor-promoting capabilities. 148,149 Cox-2 was also demonstrated to regulate p53-induced apoptosis, as the rate of cell death following DNA damage was potentiated not only in Cox-2-deficient cells, but also in cells in which Cox-2 activity was suppressed by non-steroidal anti-inflammatory drugs such as NS-398 and celecoxib.…”

Section: P53 and Mitogen-activated Protein Kinasesmentioning

confidence: 99%

The dark side of a tumor suppressor: anti-apoptotic p53

2008

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Depending on multiple factors DNA damage leads either to cell cycle arrest or apoptosis. One of the main players deciding the fate of a cell is the tumor suppressor p53 that modulates these responses in a transcription-dependent and -independent manner. Over the past few years, however, strong evidence accumulated that p53 engages also powerful pro-survival pathways by transcriptionally activating a multitude of genes whose products efficiently counteract apoptosis. Our review summarizes the current knowledge concerning approximately forty p53-regulated proteins that exert their anti-apoptotic potential by interfering with diverse cellular processes. These activities are surely essential for normal development and maintenance of a healthy organism, but may easily turn into the dark side of the tumor suppressor p53 contributing to tumorigenesis.

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Transcriptional activation of cyclooxygenase-2 by tumor suppressor p53 requires nuclear factor-kappaB

Cited by 53 publications

References 50 publications

Deregulated expression of pro-survival and pro-apoptotic p53-dependent genes upon Elongator deficiency in colon cancer cells

Deregulated expression of pro-survival and pro-apoptotic p53-dependent genes upon Elongator deficiency in colon cancer cells

Gallotannin regulates apoptosis and COX-2 expression via Akt and p38kinase pathway in human lung cancer cell line, A549

The dark side of a tumor suppressor: anti-apoptotic p53

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