Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi

Mózsik, László; Iacovelli, Riccardo; Bovenberg, Roel A. L.; Driessen, Arnold J.M.

doi:10.3389/fbioe.2022.901037

Cited by 21 publications

(21 citation statements)

References 220 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5 c). Selection of platform strains has been extensively covered by Mózsik et al., 2022 [ 204 ], numerating genotypes created through either random mutagenesis, or rational editing to allow HR-mediated targeted gene integration and easy selection. Most widely used heterologous hosts for fungal BGCs activation are Saccharomyces cerevisiae and Aspergillus species [ 36 ].…”

Section: Synthetic Biology Toolkitmentioning

confidence: 99%

Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability

Jo¹,

Zhang²,

Tam³

et al. 2023

Materials Today Bio

View full text Add to dashboard Cite

Section: Synthetic Biology Toolkitmentioning

confidence: 99%

Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability

Jo¹,

Zhang²,

Tam³

et al. 2023

Materials Today Bio

View full text Add to dashboard Cite

“…The secondary metabolites contained therein include pyranone, alkaloids (purolles and pyridones), amides, cyclopeptides, polyketide (asperyellone), and sterols. 50,64,65 The content, especially the alkaloid group, is known to have the ability as an antioxidant and antibacterial. [66][67][68][69] The solvent used in this research is ethyl acetate which is a semipolar solvent.…”

Section: Molecular Identification Of Endophytic Fungimentioning

confidence: 99%

Antioxidant and Antibacterial Activity of Endophytic Fungi Isolated from The Leaves of Sungkai (Peronema canescens)

2023

TJNPR

View full text Add to dashboard Cite

Sungkai (Peronema canescens) is a medicinal plant widely used in Indonesia. Its leaves are believed to have the potential to treat fever and boost the immune system. This study investigated the antioxidant and antibacterial activity of endophytic fungi extracts obtained from Sungkai leaves. This will provide basic information for developing the potential of natural ingredients as antioxidants and antibacterials. Endophytic fungi residing in Sungkai leaves were identified morphologically. The antioxidant test was completed using DPPH, while the antibacterial activity was tested using the paper disk diffusion method. Most potential endophytic fungal isolates were identified through molecular identification, and the isolation of a bioactive compound was achieved using column chromatography. The structure of the compound was determined using 1D and 2D NMR spectroscopy methods. Four endophytic fungi isolates were observed in the Sungkai leaves (code RND1–RND4). RND3 demonstrated very strong antioxidant activity (IC50 < 20 µg/mL) and strong antibacterial activity. Based on the molecular test, RND3 was identified as Aspergillus niger. The pure compound isolated from RND3 revealed weak and strong antioxidant (IC50 > 500 µg/mL) and antibacterial (MIC ≤ 64 µg/mL) properties, respectively. Spectroscopic analysis showed that compound 1 was 3-benzyl-2,6-dihydroxy-1,4,11,13-tetramethyl-5- methylene-12,15-dioxo-14-oxabicycloheptadeca-8,16-diene-7-carboxylic acid. Through further research, this study can be used as a basis for the development of compounds as raw materials for drugs.

show abstract

“…Mostly such a variation may be due to hindrance in the aflatoxin biosynthesis genes or belong to other non non-aflatoxin producing species. The sequencing of aflatoxin biosynthesis gene cluster was extensively studied in order to understand the mechanism and biosynthesis pathway of aflatoxin in aflatoxigenic fungi 20 , 21 . The presence of these genes is required by the Aspergillus spp.…”

Section: Introductionmentioning

confidence: 99%

Whole genome sequencing and annotation of Aspergillus flavus JAM-JKB-B HA-GG20

Bharose,

Hajare,

Narayanrao

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Groundnuts are mostly contaminated with the mold Aspergillus flavus which produces a carcinogenic mycotoxin called as aflatoxin. It is very important to understand the genetic factors underlying its pathogenicity, regulation, and biosynthesis of secondary metabolites and animal toxicities, but it still lacks useful information due to certain gaps in the era of modern technology. Therefore, the present study was considered to determine the key genes and metabolites involved in the biosynthesis of aflatoxin by using a molecular approach in a virulent strain of Aspergillus. The whole genome sequence of highly toxic and virulent Aspergillus isolates JAM-JKB-B HA-GG20 revealed 3,73,54,834 bp genome size, 2, 26, 257 number of contigs with N50 value of 49,272 bp, 12,400 genes and 48.1% of GC contained respectively. The genome sequence was compared with other known aflatoxin producing and non-producing genome of Aspergillus spp. and 61 secondary metabolite (SM) gene clusters were annotated with the toxic strain JAM-JKB-BHA-GG20 which showed similarity with other Aspergillus spp. A total number of eight genes (ver-1, AflR, pksA, uvm8, omt1, nor-1, Vha and aflP) were identified related to biosynthesis of aflatoxin and ochratoxin. Also, 69 SSR with forward and reverse primers and 137 di and tri nucleotide motifs were identified in the nucleotide sequence region related to aflatoxin gene pathway. The genes and putative metabolites identified in this study are potentially involved in host invasion and pathogenicity. As such, the genomic information obtained in this study is helpful in understanding aflatoxin gene producing pathway in comparison to other Aspergillus spp. and predicted presence of other secondary metabolites clusters viz. Nrps, T1pks etc. genes associated with a biosynthesis of OTA mycotoxin.

show abstract

Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi

Cited by 21 publications

References 220 publications

Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability

Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability

Antioxidant and Antibacterial Activity of Endophytic Fungi Isolated from The Leaves of Sungkai (Peronema canescens)

Whole genome sequencing and annotation of Aspergillus flavus JAM-JKB-B HA-GG20

Contact Info

Product

Resources

About