Transcriptional activation of a pectate lyase gene, RbPel1, during petal abscission in rose

Singh, Amar Pal; Pandey, Saurabh; Rajluxmi,; Pandey, Shveta; Nath, Paras; Sane, Aniruddha P.

doi:10.1016/j.postharvbio.2010.12.014

Cited by 26 publications

(18 citation statements)

References 32 publications

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“…In agreement with that, the expression of CitPL5 was up-regulated exclusively in the AZ-C during ethylene-promoted fruit abscission. CitPL19 was up-regulated in both the AZ-C and the FR and was closely related to PLs that were also up-regulated in the AZ of rose ( RbPEL1 ; Singh et al, 2011 ) and Arabidopsis ( AtPLL25 and AtPLL26 ; Lashbrook and Cai, 2008 ; Sun and Van Nocker, 2010 ). Furthermore, CitPL19 was previously reported to be up-regulated exclusively in the citrus LAZ (Agustí et al, 2008 , 2009 ), suggesting a role in fruit and leaf abscission but also in cell wall degradation events taking place in the FR.…”

Section: Resultsmentioning

confidence: 97%

“…Pectate lyases (PLs; EC 4.2.2.2) belong to pectate lyase family 1 (PL1; CAZy; http://www.cazy.org ; Henrissat, 1991 ; Lombard et al, 2014 ) and are classified in five different groups (Groups I, II, III, IV, and V; Sun and Van Nocker, 2010 ). Group I of PLs contained all enzymes previously reported to be induced during organ abscission in Arabidopsis ( AtPLL18, AtPLL19, AtPLL22, AtPLL23, AtPLL24 , and AtPLL25 ; Lashbrook and Cai, 2008 ; Niederhuth et al, 2013 ), soybean ( GmPL01 and GmPL02 ; Tucker et al, 2007 ), banana ( MaPEL1 ; Mbéguié-a-Mbéguié et al, 2009 ), and rose ( RbPEL1 ; Singh et al, 2011 ; Figure S5 ). In agreement with that, the expression of CitPL5 was up-regulated exclusively in the AZ-C during ethylene-promoted fruit abscission.…”

Section: Resultsmentioning

confidence: 99%

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Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus

et al. 2017

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Abscission is a cell separation process by which plants can shed organs such as fruits, leaves, or flowers. The process takes place in specific locations termed abscission zones. In fruit crops like citrus, fruit abscission represents a high percentage of annual yield losses. Thus, understanding the molecular regulation of abscission is of capital relevance to control production. To identify genes preferentially expressed within the citrus fruit abscission zone (AZ-C), we performed a comparative transcriptomics assay at the cell type resolution level between the AZ-C and adjacent fruit rind cells (non-abscising tissue) during ethylene-promoted abscission. Our strategy combined laser microdissection with microarray analysis. Cell wall modification-related gene families displayed prominent representation in the AZ-C. Phylogenetic analyses of such gene families revealed a link between phylogenetic proximity and expression pattern during abscission suggesting highly conserved roles for specific members of these families in abscission. Our transcriptomic data was validated with (and strongly supported by) a parallel approach consisting on anatomical, histochemical and biochemical analyses on the AZ-C during fruit abscission. Our work identifies genes potentially involved in organ abscission and provides relevant data for future biotechnology approaches aimed at controlling such crucial process for citrus yield.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus

et al. 2017

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“…The analysis revealed that the tomato genome carried all genes orthologous to those described previously in Arabidopsis and the tomato PL proteins could be divided into five subfamilies (Figure a), consistent with a previous investigation by Singh et al . (). Alignment of predicted PL domain sequences showed that three typical motifs, motif 1 (WIDH), motif 2 (DGLIDAIMASTAITISNNYF) and motif 3 (LIQRMPRCRHGYFHVVNNDY) could be recognized in the sequence (Figure b), thus further confirming the identity of the predicted PLs in tomato.…”

Section: Resultsmentioning

confidence: 97%

Silencing of SlPL, which encodes a pectate lyase in tomato, confers enhanced fruit firmness, prolonged shelf‐life and reduced susceptibility to grey mould

Lü

Huang

Xiong

et al. 2017

Plant Biotechnology Journal

164

110

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SummaryPectate lyase genes have been documented as excellent candidates for improvement of fruit firmness. However, implementation of pectate lyase in regulating fruit postharvest deterioration has not been fully explored. In this report, 22 individual pectate lyase genes in tomato were identified, and one pectate lyase gene SlPL (Solyc03g111690) showed dominant expression during fruit maturation. RNA interference of SlPL resulted in enhanced fruit firmness and changes in pericarp cells. More importantly, the SlPL‐RNAi fruit demonstrated greater antirotting and pathogen‐resisting ability. Compared to wild‐type, SlPL‐RNAi fruit had higher levels of cellulose and hemicellulose, whereas the level of water‐soluble pectin was lower. Consistent with this, the activities of peroxidase, superoxide dismutase and catalase were higher in SlPL‐RNAi fruit, and the malondialdehyde concentration was lower. RNA‐Seq results showed large amounts of differentially expressed genes involved in hormone signalling, cell wall modification, oxidative stress and pathogen resistance. Collectively, these data demonstrate that pectate lyase plays an important role in both fruit softening and pathogen resistance. This may advance knowledge of postharvest fruit preservation in tomato and other fleshy fruit.

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“…The expression levels of most SIPs did not change during petal development. The expression of unigenes annotated as genes involved in cell wall synthesis, modification or hydrolysis, such as cellulose synthase (CES), xylosidase (XYL), pectin esterase (PE), polygalacturonase (PG), and pectate lyase (PL) 17 18 19 20 , were also assessed, as these may participate in rose petal expansion ( Fig 5 , Supplementary Table S11 ). The expression of 10 CESs, three XYLs, four PEs, seven PLs, and six PGs in OF_PP petals was more than twice as high as those in the FB_PP stage, with particularly marked differences in the expression of the PLs.…”

Section: Resultsmentioning

confidence: 99%

Comparative RNA-seq analysis of transcriptome dynamics during petal development in Rosa chinensis

Han¹,

Wan²,

Cheng³

et al. 2017

Sci Rep

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The developmental process that produces the ornate petals of the China rose (Rosa chinensis) is complex and is thought to depend on the balanced expression of a functionally diverse array of genes; however, the molecular basis of rose petal development is largely unknown. Here, petal growth of the R. chinensis cultivar ‘Old Blush’ was divided into four developmental stages, and RNA-seq technology was used to analyse the dynamic changes in transcription that occur as development progresses. In total, 598 million clean reads and 61,456 successfully annotated unigenes were obtained. Differentially expressed gene (DEG) analysis comparing the transcriptomes of the developmental stages resulted in the identification of several potential candidate genes involved in petal development. DEGs involved in anthocyanin biosynthesis, petal expansion, and phytohormone pathways were considered in depth, in addition to several candidate transcription factors. These results lay a foundation for future studies on the regulatory mechanisms underlying rose petal development and may be used in molecular breeding programs aimed at generating ornamental rose lines with desirable traits.

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Transcriptional activation of a pectate lyase gene, RbPel1, during petal abscission in rose

Cited by 26 publications

References 32 publications

Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus

Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus

Silencing of SlPL, which encodes a pectate lyase in tomato, confers enhanced fruit firmness, prolonged shelf‐life and reduced susceptibility to grey mould

Comparative RNA-seq analysis of transcriptome dynamics during petal development in Rosa chinensis

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