Transcription regulatory signals in the 5′ and 3′ regions of Oreochromis aureus ER gene

Tan, Nguan Soon; Lam, T.J.; Ding, Jeak Ling

doi:10.1016/s0303-7207(96)03911-1

Cited by 5 publications

(2 citation statements)

References 31 publications

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“…Interestingly, the recent characterization of a genomic clone encoding the ER from the fish Oreochromis aureus revealed the presence of a functional ERE in the leading exon, as well as a TGACCC half-site that could form a DR-25 response element with the downstream ERE half-site (63,64). Comparison of the O. aureus sequence with that of the rtER gene showed a very high homology between the sequences and the spacings of the nuclear receptor binding sites (Fig.…”

Section: Discussionmentioning

confidence: 96%

The Nuclear Orphan Receptors COUP-TF and ARP-1 Positively Regulate the Trout Estrogen Receptor Gene through Enhancing Autoregulation

Lazennec

Kern

Valotaire

et al. 1997

Molecular and Cellular Biology

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The rainbow trout estrogen receptor (rtER) is a positively autoregulated gene in liver cells. In a previous report, we showed that upregulation is mediated by an estrogen response element (ERE) located in the proximal promoter of the gene and that a half binding site for nuclear receptors (5-TGACCT-3) located 15 bp upstream of the ERE is involved in the magnitude of the estrogen response. We now report that the human orphan receptor COUP-TF and a COUP-TF-like protein from trout liver are able to bind to the consensus half-site. When cotransfected with the rtER gene proximal promoter, COUP-TF had no regulatory functions on its own. Interestingly, COUP-TF enhanced rtER transactivation properties in the presence of estradiol in a dose-dependent manner when cotransfected with the rtER gene promoter. Unliganded retinoid receptor heterodimers had the same helper function as COUP-TF in the presence of estradiol but were switched to repressors when the ligand all-trans-retinoic acid was added. Mutation of the consensus half-site only slightly reduced COUP-TF helper function, suggesting that it actually results from a complex mechanism that probably involves both DNA binding of COUP-TF to the promoter and protein-protein interaction with another transcription factor bound to the promoter. Nevertheless, a DNA-binding-defective mutant of COUP-TF was also defective in ER helper function. Competition footprinting analysis suggested that COUP-TF actually establishes contacts with the consensus upstream half-site and the downstream ERE half-site that would form a DR-24-like response element. Interaction of COUP-TF with the DR-24 element was confirmed in footprinting assays by using nuclear extracts from Saccharomyces cerevisiae expressing COUP-TF. Finally, interaction of COUP-TF with mutants of the rtER gene promoter showed that COUP-TF recognizes the ERE when the upstream half-site is mutated. These data show that COUP-TF may activate transcription through interaction with other nuclear receptors. This cross-talk between liganded nuclear receptors and orphan receptors is likely to modulate the spectrum of action of a particular ligand-receptor complex and may participate in the cell-type specificity of the ligand effect.

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Section: Discussionmentioning

confidence: 96%

The Nuclear Orphan Receptors COUP-TF and ARP-1 Positively Regulate the Trout Estrogen Receptor Gene through Enhancing Autoregulation

Lazennec

Kern

Valotaire

et al. 1997

Molecular and Cellular Biology

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“…Forty‐eight hours after induction, cell extracts were assayed for CAT enzyme using CAT ELISA (Boehringer Mannheim). SEAP activity was measured as described by Tan et al [10]. The data presented represent the means±S.D.…”

Section: Methodsmentioning

confidence: 99%

Synergistic effects of nuclear factors – GATA, VBP and ER in potentiating vitellogenin gene transcription

et al. 1999

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The Oreochromis aureus vitellogenin (OaVtg) gene contains three imperfect oestrogen response elements (EREs) and GATA and VBP (vitellogenin binding protein) binding sites. An analysis of the promoter indicates that the 5P P-flanking region up to position 3 3625 is sufficient to mediate E 2 control. Furthermore, transfection of deletion and mutagenised promoters indicates that both GATA and VBP synergise with ER, and thus contribute to the regulation of the endogenous OaVtg gene. These findings support the notion that the interplay of promoter elements mediates proper hormone-dependent and tissue-specific expression of the OaVtg gene, regardless of non-consensus sequence context of EREs and VBP.z 1999 Federation of European Biochemical Societies.

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A novel piscine vitellogenin gene: structural and functional analyses of estrogen-inducible promoter

Teo

Tan

Lim

et al. 1998

Molecular and Cellular Endocrinology

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Transcription regulatory signals in the 5′ and 3′ regions of Oreochromis aureus ER gene

Cited by 5 publications

References 31 publications

The Nuclear Orphan Receptors COUP-TF and ARP-1 Positively Regulate the Trout Estrogen Receptor Gene through Enhancing Autoregulation

The Nuclear Orphan Receptors COUP-TF and ARP-1 Positively Regulate the Trout Estrogen Receptor Gene through Enhancing Autoregulation

Synergistic effects of nuclear factors – GATA, VBP and ER in potentiating vitellogenin gene transcription

A novel piscine vitellogenin gene: structural and functional analyses of estrogen-inducible promoter

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