Transcription regulation strategies in methylotrophs: progress and challenges

Abstract

As a promising industrial microorganism, methylotroph is capable of using methane or methanol as the sole carbon source natively, which has been utilized in the biosynthesis of various bioproducts. However, the relatively low efficiency of carbon conversion has become a limiting factor throughout the development of methanotrophic cell factories due to the unclear genetic background. To better highlight their advantages in methane or methanol-based biomanufacturing, some metabolic engineering strategies, including upstream transcription regulation projects, are being popularized in methylotrophs. In this review, several strategies of transcription regulations applied in methylotrophs are summarized and their applications are discussed and prospected.

Production of Bacillus subtillis protein mass on the microbial mass of Methylococcus capsulatus

“…However, the industrial use of such a method of microbiological synthesis, according to X. Huang et al (2022), is limited by the relatively low efficiency of carbon processing by methanotrophic cells. For this purpose, the studies conducted utilised symbiotic microorganisms, in particular the culture of the probiotic bacteria Bacillus subtillis.…”

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“…However, the industrial use of such a method of microbiological synthesis, according to X. Huang et al (2022), is limited by the relatively low efficiency of carbon processing by methanotrophic cells. For this purpose, the studies conducted utilised symbiotic microorganisms, in particular the culture of the probiotic bacteria Bacillus subtillis.…”