Transcription of the chicken anemia virus (CAV) genome and synthesis of its 52-kDa protein

Transcriptional analysis and genome expression of chicken anaemia virus

“…2 b). This result contrasts with that reported by Noteborn et al (1992) in which S~ nuclease protection analysis located the 3' end of the major transcript 25 nt downstream from the poly(A) addition signal.…”

“…2 a) was located at nt position 360 (A residue) of the CAV sequence. In agreement with Noteborn et al (1992), this places the 5' end of the major transcript 30 nt downstream of the TATA box at nt positions 329 to 325, and 25 nt upstream from the initiation codon of the first major ORF at nt positions 386 to 388 of the CAV sequence (Meehan et at., 1992).…”

“…3 a). Given the map positions of the 3' and 5' termini, these results support the view that the major 2-0 kb transcript is an unspliced message (Noteborn et al, 1992), Although sequence analysis suggests promoter activity is associated with the CAV non-transcribed region, and the region is rich in sequences of putative regulatory significance, no evidence of promoter activity has yet been demonstrated.…”

TAT-apoptin is efficiently delivered and induces apoptosis in cancer cells

“…Apoptin is a 13.6 kDa, serine and threonine rich protein which consists of 121 amino acids (aa) containing two positively charged and two proline rich domains (Noteborn et al, 1991(Noteborn et al, , 1994. Apoptin-induced apoptosis occurs independently of the tumour-suppressor gene p53 (Zhuang et al, 1995c).…”

Broyler damızlık ve ticari broyler sürülerinde tavuk anemi virüsünün ve antikor varlığının araştırılması

“…Gen sekansı belirlenen izolatlardan CAA 82-2 hariç hepsi VP1, VP2 ve VP3 proteinleri için 3 parçalı üst üste binmiş açık okuma alanı (Open Reading Frame-ORF) kod parçalarına sahiptir. ORF 1 (pozisyon 853-2200nt), VP1 (51.6kDa, 499AA) yapısal kapsid proteinini kodlar, ORF2 (pozisyon 380-1028 nt) kapsid oluşumu ile ilişkili olan VP2 (24kDa, 216AA) proteinini kodlar, ORF3 (pozisyon 486-849nt) yapısal olmayan VP3 (13.6kDa, 121 AA) proteinini kodlar (12,15,19). Noteborn ve ark.…”