Transcription of spoIVB is the only role of sigma G that is essential for pro-sigma K processing during spore formation in Bacillus subtilis

Gomez, Manuel; Cutting, Simon M.; Stragier, Patrick

doi:10.1128/jb.177.16.4825-4827.1995

Cited by 36 publications

(36 citation statements)

References 17 publications

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“…Interestingly, pro-aK is actually synthesized approximately 60 min prior to processing (t,), yet the earliest possible time at which cleavage can occur is 30 min after synthesis of pro-aH ( t 3 J . This delay in processing, even when SpoIVFB is constitutively active, has been attributed to the time required for the putative processing complex to assemble into the outer forespore membranes (Gomez et al, 1995). In contrast, the 60fC mutations introduced a substantial delay of about 3 h before aK-directed gene expression commenced (t,-J. This delayed aK-dependent gene expression implies that the 6ofC mutations (or the absence of BofC) do not facilitate constitutive activity of SpoIVFB.…”

Section: Timing Of Crk Activation In Spolllga1 Cellsmentioning

confidence: 94%

bofC Encodes a Putative Forespore Regulator of the Bacillus Subtilis σk Checkpoint

Gomez

Cutting

1997

Microbiology

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Section: Timing Of Crk Activation In Spolllga1 Cellsmentioning

confidence: 94%

bofC Encodes a Putative Forespore Regulator of the Bacillus Subtilis σk Checkpoint

Gomez

Cutting

1997

Microbiology

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“…SpoIVFB is presumably activated by the release of SpoIVFA and BofA-mediated inhibition in response to a signal received from the forespore. This intercompartmental signal consists of the action of only one Gdependent gene product, the forespore-expressed SpoIVB protein (Cutting et al, 1991a;Gomez et al, 1995;Van Hoy and Hoch, 1990). The importance of the K checkpoint is clearly illustrated when K is rendered active without the need for proteolytic cleavage.…”

Section: Introductionmentioning

confidence: 99%

SpoIVB has two distinct functions during spore formation in Bacillus subtilis

Oke

Shchepetov

Cutting

1997

Molecular Microbiology

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SummaryThe Bacillus subtilis SpoIVB protein is a critical component of the intercompartmental signal-transduction pathway that activates the sigma factor, K , in the mother cell of the sporulating cell. SpoIVB, synthesized in the forespore chamber, must act across two layers of phospholipid membrane to facilitate proteolytic processing of inactive pro-K to active K . We have used a genetic approach to dissect SpoIVB function and found that this protein has two distinct developmental functions. One function is that of intercompartmental signalling of pro-K processing. The other role is essential to spore formation and is illustrated by mutations of SpoIVB which allow cell-cell signalling of pro-K processing but prevent the formation of viable spores. Using localized and sitespecific mutagenesis we have identified a functional domain of SpoIVB that is involved in its non-signalling role.

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“…Disruption of spoIVB blocks pro-K processing, and transcription of spoIVB is largely dependent upon G (39,96). Transcription of spoIVB is the only function of G that is required for pro-K processing (95). The SpoIVB protein has a serine peptidase domain and a PDZ domain for protein-protein interactions; both domains are important for pro-K processing and sporulation (116,297).…”

mentioning

confidence: 99%

Compartmentalization of Gene Expression duringBacillus subtilisSpore Formation

Hilbert

Piggot

2004

Microbiol Mol Biol Rev

318

432

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Gene expression in members of the family Bacillaceae becomes compartmentalized after the distinctive, asymmetrically located sporulation division. It involves complete compartmentalization of the activities of sporulation-specific sigma factors, σF in the prespore and then σE in the mother cell, and then later, following engulfment, σG in the prespore and then σK in the mother cell. The coupling of the activation of σF to septation and σG to engulfment is clear; the mechanisms are not. The σ factors provide the bare framework of compartment-specific gene expression. Within each σ regulon are several temporal classes of genes, and for key regulators, timing is critical. There are also complex intercompartmental regulatory signals. The determinants for σF regulation are assembled before septation, but activation follows septation. Reversal of the anti-σF activity of SpoIIAB is critical. Only the origin-proximal 30% of a chromosome is present in the prespore when first formed; it takes ≈15 min for the rest to be transferred. This transient genetic asymmetry is important for prespore-specific σF activation. Activation of σE requires σF activity and occurs by cleavage of a prosequence. It must occur rapidly to prevent the formation of a second septum. σG is formed only in the prespore. SpoIIAB can block σG activity, but SpoIIAB control does not explain why σG is activated only after engulfment. There is mother cell-specific excision of an insertion element in sigK and σE-directed transcription of sigK, which encodes pro-σK. Activation requires removal of the prosequence following a σG-directed signal from the prespore

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Transcription of spoIVB is the only role of sigma G that is essential for pro-sigma K processing during spore formation in Bacillus subtilis

Cited by 36 publications

References 17 publications

bofC Encodes a Putative Forespore Regulator of the Bacillus Subtilis σk Checkpoint

bofC Encodes a Putative Forespore Regulator of the Bacillus Subtilis σk Checkpoint

SpoIVB has two distinct functions during spore formation in Bacillus subtilis

Compartmentalization of Gene Expression duringBacillus subtilisSpore Formation

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