Transcription of<i>ST2A</i>Encoding A Sulfotransferase Family Protein That Is Involved in Jasmonic Acid Metabolism Is Controlled According to the Circadian Clock- and PIF4/PIF5-Mediated External Coincidence Mechanism in<i>Arabidopsis thaliana</i>

Yamashino, Takafumi; Kitayama, Miki; Mizuno, Takeshi

doi:10.1271/bbb.130559

Cited by 18 publications

(13 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, five of the genes that were upregulated in hps5 are involved in jasmonate (JA) biosynthesis or signalling. These genes encode phospholipase A 2A (PLA2A) [ 41 ], sulfotransferase 2A (ST2A) [ 42 ], lipoxygenase 2 (LOX2) [ 43 ], salt tolerance zinc finger (ZAT10) [ 44 ], and octadecanoid-responsive AP2/ERF59 (ORA59) [ 45 ]. This suggests that enhanced ethylene signaling might upregulate JA biosynthesis and signalling.…”

Section: Resultsmentioning

confidence: 99%

The Molecular Mechanism of Ethylene-Mediated Root Hair Development Induced by Phosphate Starvation

et al. 2016

View full text Add to dashboard Cite

Enhanced root hair production, which increases the root surface area for nutrient uptake, is a typical adaptive response of plants to phosphate (Pi) starvation. Although previous studies have shown that ethylene plays an important role in root hair development induced by Pi starvation, the underlying molecular mechanism is not understood. In this work, we characterized an Arabidopsis mutant, hps5, that displays constitutive ethylene responses and increased sensitivity to Pi starvation due to a mutation in the ethylene receptor ERS1. hps5 accumulates high levels of EIN3 protein, a key transcription factor involved in the ethylene signaling pathway, under both Pi sufficiency and deficiency. Pi starvation also increases the accumulation of EIN3 protein. Combined molecular, genetic, and genomic analyses identified a group of genes that affect root hair development by regulating cell wall modifications. The expression of these genes is induced by Pi starvation and is enhanced in the EIN3-overexpressing line. In contrast, the induction of these genes by Pi starvation is suppressed in ein3 and ein3eil1 mutants. EIN3 protein can directly bind to the promoter of these genes, some of which are also the immediate targets of RSL4, a key transcription factor that regulates root hair development. Based on these results, we propose that under normal growth conditions, the level of ethylene is low in root cells; a group of key transcription factors, including RSL4 and its homologs, trigger the transcription of their target genes to promote root hair development; Pi starvation increases the levels of the protein EIN3, which directly binds to the promoters of the genes targeted by RSL4 and its homologs and further increase their transcription, resulting in the enhanced production of root hairs. This model not only explains how ethylene mediates root hair responses to Pi starvation, but may provide a general mechanism for how ethylene regulates root hair development under both stress and non-stress conditions.

show abstract

Section: Resultsmentioning

confidence: 99%

The Molecular Mechanism of Ethylene-Mediated Root Hair Development Induced by Phosphate Starvation

et al. 2016

View full text Add to dashboard Cite

show abstract

“…For example, transcripts involved in JA biosynthesis/signaling ( JAR1 , LOX2 , WRKY40 , WRKY50 , WRKY70 ) mainly had decreased abundance (Table 5 ) but a significant increase in abundance of ST2A and CYP94B1 was observed in response to glyphosate. ST2A encodes a hydroxyjasmonate sulfotransferase in Arabidopsis, which inactivates the function of JA [ 39 ]. Additionally, transcripts involved in ethylene biosynthesis (see Additional file 6 ) had decreased ( ACS1 , ACS6 , ACS10 ) and increased ( ACS11 ) abundance (Table 5 ) in subsequent generations of aerial tissue derived from crown buds of glyphosate-treated plants.…”

Section: Resultsmentioning

confidence: 99%

Glyphosate’s impact on vegetative growth in leafy spurge identifies molecular processes and hormone cross-talk associated with increased branching

et al. 2015

View full text Add to dashboard Cite

BackgroundLeafy spurge (Euphorbia esula) is a perennial weed that is considered glyphosate tolerant, which is partially attributed to escape through establishment of new vegetative shoots from an abundance of underground adventitious buds. Leafy spurge plants treated with sub-lethal concentrations of foliar-applied glyphosate produce new vegetative shoots with reduced main stem elongation and increased branching. Processes associated with the glyphosate-induced phenotype were determined by RNAseq using aerial shoots derived from crown buds of glyphosate-treated and -untreated plants. Comparison between transcript abundance and accumulation of shikimate or phytohormones (abscisic acid, auxin, cytokinins, and gibberellins) from these same samples was also done to reveal correlations.ResultsTranscriptome assembly and analyses confirmed differential abundance among 12,918 transcripts (FDR ≤ 0.05) and highlighted numerous processes associated with shoot apical meristem maintenance and stem growth, which is consistent with the increased number of actively growing meristems in response to glyphosate. Foliar applied glyphosate increased shikimate abundance in crown buds prior to decapitation of aboveground shoots, which induces growth from these buds, indicating that 5-enolpyruvylshikimate 3-phosphate (EPSPS) the target site of glyphosate was inhibited. However, abundance of shikimate was similar in a subsequent generation of aerial shoots derived from crown buds of treated and untreated plants, suggesting EPSPS is no longer inhibited or abundance of shikimate initially observed in crown buds dissipated over time. Overall, auxins, gibberellins (precursors and catabolites of bioactive gibberellins), and cytokinins (precursors and bioactive cytokinins) were more abundant in the aboveground shoots derived from glyphosate-treated plants.ConclusionBased on the overall data, we propose that the glyphosate-induced phenotype resulted from complex interactions involving shoot apical meristem maintenance, hormone biosynthesis and signaling (auxin, cytokinins, gibberellins, and strigolactones), cellular transport, and detoxification mechanisms.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1627-9) contains supplementary material, which is available to authorized users.

show abstract

“…Probably, it inactivates the function of jasmonic acids and therefore enhances the hypocotyl growth ( Gidda et al, 2003 ). Yamashino et al (2013) showed that AtSOT15 transcription is also regulated by an external coincidence mechanism. Database research indicated that AtSOT15 might be a target of the phytochrome interacting transcriptional factors PIF4 and PIF5.…”

Section: Expression Of Sulfotransferasesmentioning

confidence: 99%

The multi-protein family of sulfotransferases in plants: composition, occurrence, substrate specificity, and functions

Hirschmann¹,

Krause²,

Papenbrock³

2014

Front. Plant Sci.

View full text Add to dashboard Cite

All members of the sulfotransferase (SOT, EC 2.8.2.-) protein family transfer a sulfuryl group from the donor 3′-phosphoadenosine 5′-phosphosulfate (PAPS) to an appropriate hydroxyl group of several classes of substrates. The primary structure of these enzymes is characterized by a histidine residue in the active site, defined PAPS binding sites and a longer SOT domain. Proteins with this SOT domain occur in all organisms from all three domains, usually as a multi-protein family. Arabidopsis thaliana SOTs, the best characterized SOT multi-protein family, contains 21 members. The substrates for several plant enzymes have already been identified, such as glucosinolates, brassinosteroids, jasmonates, flavonoids, and salicylic acid. Much information has been gathered on desulfo-glucosinolate (dsGl) SOTs in A. thaliana. The three cytosolic dsGl SOTs show slightly different expression patterns. The recombinant proteins reveal differences in their affinity to indolic and aliphatic dsGls. Also the respective recombinant dsGl SOTs from different A. thaliana ecotypes differ in their kinetic properties. However, determinants of substrate specificity and the exact reaction mechanism still need to be clarified. Probably, the three-dimensional structures of more plant proteins need to be solved to analyze the mode of action and the responsible amino acids for substrate binding. In addition to A. thaliana, more plant species from several families need to be investigated to fully elucidate the diversity of sulfated molecules and the way of biosynthesis catalyzed by SOT enzymes.

show abstract

Transcription ofST2AEncoding A Sulfotransferase Family Protein That Is Involved in Jasmonic Acid Metabolism Is Controlled According to the Circadian Clock- and PIF4/PIF5-Mediated External Coincidence Mechanism inArabidopsis thaliana

Cited by 18 publications

References 39 publications

The Molecular Mechanism of Ethylene-Mediated Root Hair Development Induced by Phosphate Starvation

The Molecular Mechanism of Ethylene-Mediated Root Hair Development Induced by Phosphate Starvation

Glyphosate’s impact on vegetative growth in leafy spurge identifies molecular processes and hormone cross-talk associated with increased branching

The multi-protein family of sulfotransferases in plants: composition, occurrence, substrate specificity, and functions

Contact Info

Product

Resources

About