Transcription factors: Time to deliver

Ulasov, A. V.; Rosenkranz, Andrey A.; Соболев, А. С.

doi:10.1016/j.jconrel.2017.11.004

Cited by 32 publications

(24 citation statements)

References 249 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The piglet microbiotas had a significantly greater impact on TF expression in the gut than in any other the other tissues. TFs regulate and initiate gene expression, and are thus involved in most biological processes 43 . Here, TF-gene expression levels in the colon were strongly affected by the piglet microbiotas.…”

Section: Discussionmentioning

confidence: 99%

“…The jejuna, oral mucosae, spleens, and livers were less affected. Specific TFs may thus be potentially useful as pharmaceutical drug targets 43 . In GF piglets, several tumor suppressor TFs, including the TP53 gene (tumor protein p53) and interferon regulatory factor-1 ( IRF1 ), were downregulated in guts of GF piglets as compared to CV piglets, suggesting that microbiotas are essential for the restoration of tumor suppressor activity by these TFs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Gene expression profiles of germ-free and conventional piglets from the same litter

Sun

Zhong

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Germ-free (GF) pigs have clear microbiological backgrounds, and are extensively used as large animal models in the biomedical sciences. However, investigations of the transcriptomic differences between GF and cesarean-derived conventional (CV) piglets are limited. To improve our understanding of GF pigs, and to increase the utility of pigs as an alternative non-rodent model, we used RNA sequencing to profile gene expression in five tissues (the oral mucosae, jejunum, colon, liver, and spleen) of four male GF piglets and four male CV piglets from the same litter. We identified 14 genes that were differentially expressed in all five tissues. Seven of these common differentially expressed genes (DEGs) were interferon-inducible genes, and all 14 were consistently downregulated in the GF piglets as compared to the CV piglets. Compared to the other tissues tested, the expression of transcription factors (TFs) in the colon was most affected by the absence of a microbiota. The expression patterns of immune-related genes were downregulated in the GF piglets as compared to the CV piglets, indicating that the intestinal microbiota influenced gene expression in other tissues besides the gut. Gene Ontology (GO) analysis indicated that, in pigs, the intestinal microbiota affected the expression of genes related to immune system function and development.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gene expression profiles of germ-free and conventional piglets from the same litter

Sun

Zhong

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Transcription factors (TFs) play an important role in pluripotent stem cell induction and transdifferentiation[ 56 ]. Recently, they have been used to differentiate hPSCs into desired cells or tissues such as neural[ 57 ], liver[ 58 , 59 ], and cardiac muscle[ 60 , 61 ].…”

Section: Differentiation Induction From Hpscsmentioning

confidence: 99%

Practical choice for robust and efficient differentiation of human pluripotent stem cells

Fang¹,

Liu²,

Zhou³

et al. 2020

WJSC

View full text Add to dashboard Cite

Human pluripotent stem cells (hPSCs) have the distinct advantage of being able to differentiate into cells of all three germ layers. Target cells or tissues derived from hPSCs have many uses such as drug screening, disease modeling, and transplantation therapy. There are currently a wide variety of differentiation methods available. However, most of the existing differentiation methods are unreliable, with uneven differentiation efficiency and poor reproducibility. At the same time, it is difficult to choose the optimal method when faced with so many differentiation schemes, and it is time-consuming and costly to explore a new differentiation approach. Thus, it is critical to design a robust and efficient method of differentiation. In this review article, we summarize a comprehensive approach in which hPSCs are differentiated into target cells or organoids including brain, liver, blood, melanocytes, and mesenchymal cells. This was accomplished by employing an embryoid body-based three-dimensional (3D) suspension culture system with multiple cells co-cultured. The method has high stable differentiation efficiency compared to the conventional 2D culture and can meet the requirements of clinical application. Additionally, ex vivo co-culture models might be able to constitute organoids that are highly similar or mimic human organs for potential organ transplantation in the future.

show abstract

“…Subcellular delivery systems have attracted growing attention of researchers (Jhaveri and Torchilin, 2016; Ulasov et al, 2018). There are several groups of drugs that can be efficiently delivered using these systems.…”

Section: Introductionmentioning

confidence: 99%

“…This review focuses on MNT delivery of AEEs; delivery of photosensitizers and other molecules by MNTs has been reviewed earlier (Sharman et al, 2004; Glover, 2012; Simoes et al, 2015; Slastnikova et al, 2015; Ulasov et al, 2018). …”

Section: Introductionmentioning

confidence: 99%

Modular Nanotransporters for Nuclear-Targeted Delivery of Auger Electron Emitters

Соболев

2018

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

This review describes artificial modular nanotransporters (MNTs) delivering their cargos into target cells and then into the nuclei – the most vulnerable cell compartment for most anticancer agents and especially for radionuclides emitting short-range particles. The MNT strategy uses natural subcellular transport processes inherent in practically all cells including cancer cells. The MNTs use these processes just as a passenger who purchased tickets for a multiple-transfer trip making use of different kinds of public transport to reach the desired destination. The MNTs are fusion polypeptides consisting of several parts, replaceable modules, accomplishing binding to a specific receptor on the cell and subsequent internalization, endosomal escape and transport into the cell nucleus. Radionuclides emitting short-range particles, like Auger electron emitters, acquire cell specificity and significantly higher cytotoxicity both in vitro and in vivo when delivered by the MNTs into the nuclei of cancer cells. MNT modules are interchangeable, allowing replacement of receptor recognition modules, which permits their use for different types of cancer cells and, as a cocktail of several MNTs, for targeting several tumor-specific molecules for personalized medicine.

show abstract

Transcription factors: Time to deliver

Cited by 32 publications

References 249 publications

Gene expression profiles of germ-free and conventional piglets from the same litter

Gene expression profiles of germ-free and conventional piglets from the same litter

Practical choice for robust and efficient differentiation of human pluripotent stem cells

Modular Nanotransporters for Nuclear-Targeted Delivery of Auger Electron Emitters

Contact Info

Product

Resources

About