Transcription factors and ovarian functions

Sirotkin, Alexander V.

doi:10.1002/jcp.22248

Cited by 27 publications

(13 citation statements)

References 57 publications

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“…In this context, the first thing to look at is the modulation of transcription factors that control cell fate. The first obvious observation is the initial rise and then decrease of several transcription factors associated with follicular differentiation: CDKN2A (cell division inhibition, Huang et al Figure 2), smad family member 7 (SMAD7), is predicted to be activated specifically from 44 to 68 h and inhibited from 20 to 44 h and from 68 to 92 h. Only two transcription factors, STAT1 and CCAAT/enhancer binding protein, alpha (CEBPA), are predicted to be inhibited specifically from 20 to 44 h and from 68 to 92 h. The transcription factors TP53, CREB binding protein (CREBBP), smad family member 4 (SMAD4), and forkhead box O3 (FOXO3) seem specific to the competence-acquisition period (20-44 h), and three of them (TP53, CREBBP, and FOXO3) are implicated in the mediation of the effect of hormones in the ovary (Sirotkin 2011). TP53 is upstream of the selected candidates TFPI2, IGF2, NRP1, and ANXA1, which will be addressed below.…”

Section: Gene Analysismentioning

confidence: 99%

Changes in granulosa cells' gene expression associated with increased oocyte competence in bovine

Nivet¹,

Vigneault²,

Blondin³

et al. 2013

Reproduction

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One of the challenges in mammalian reproduction is to understand the basic physiology of oocyte quality. It is believed that the follicle status is linked to developmental competence of the enclosed oocyte. To explore the link between follicles and competence in cows, previous research at our laboratory has developed an ovarian stimulation protocol that increases and then decreases oocyte quality according to the timing of oocyte recovery post-FSH withdrawal (coasting). Using this protocol, we have obtained the granulosa cells associated with oocytes of different qualities at selected times of coasting. Transcriptome analysis was done with Embryogene microarray slides and validation was performed by real-time PCR. Results show that the major changes in gene expression occurred from 20 to 44 h of coasting, when oocyte quality increases. Secondly, among upregulated genes (20-44 h), 25% were extracellular molecules, highlighting potential granulosa signaling cascades. Principal component analysis identified two patterns: one resembling the competence profile and another associated with follicle growth and atresia. Additionally, three major functional changes were identified: i) the end of follicle growth (BMPR1B, IGF2, and RELN), involving interactions with the extracellular matrix (TFPI2); angiogenesis (NRP1), including early hypoxia, and potentially oxidative stress (GFPT2, TF, and VNN1) and ii) apoptosis (KCNJ8) followed by iii) inflammation (ANKRD1). This unique window of analysis indicates a progressive hypoxia during coasting mixed with an increase in apoptosis and inflammation. Potential signaling pathways leading to competence have been identified and will require downstream testing. This preliminary analysis supports the potential role of the follicular differentiation in oocyte quality both during competence increase and decrease phases.Reproduction (2013) 145 555-565

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Section: Gene Analysismentioning

confidence: 99%

Changes in granulosa cells' gene expression associated with increased oocyte competence in bovine

Nivet¹,

Vigneault²,

Blondin³

et al. 2013

Reproduction

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“…These changes are accomplished by spatio-temporally regulated expression of genes in periovulatory follicles. Specific transcription factors induced by the LH surge directly control the dynamic changes in periovulatory gene expression, thus playing a central role in successful ovulation of COCs and luteal transformation [reviewed in (Richards, 2007; Russell and Robker, 2007; Sirotkin, 2010; Stouffer et al, 2007)].…”

Section: Introductionmentioning

confidence: 99%

The role for runt related transcription factor 2 (RUNX2) as a transcriptional repressor in luteinizing granulosa cells

Park

Franceschi

et al. 2012

Molecular and Cellular Endocrinology

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Transcription factors induced by the LH surge play a vital role in reprogramming the gene expression in periovulatory follicles. The present study investigated the role of RUNX2 transcription factor in regulating the expression of Runx1, Ptgs2, and Tnfaip6 using cultured granulosa cells isolated from PMSG-primed immature rats. hCG or forskolin+PMA induced the transient increase in Runx1, Ptgs2, and Tnfaip6 expression, while the expression of Runx2 continued to increase until 48 h. The knockdown of the agonist-stimulated Runx2 expression increased Runx1, Ptgs2, and Tnfaip6 expression and PGE2 levels in luteinizing granulosa cells. Conversely, the over-expression of RUNX2 inhibited the expression of these genes and PGE2 levels. The mutation of RUNX binding motifs in the Runx1 promoter enhanced transcriptional activity of the Runx1 promoter. The knockdown and overexpression of Runx2 increased and decreased Runx1 promoter activity, respectively. ChlP assays revealed the binding of RUNX2 in the Runx1 and Ptgs2 promoters. Together, these novel findings provide support for the role of RUNX2 in down-regulation of Runx1, Ptgs2, and Tnfaip6 during the late ovulatory period to support proper ovulation and/or luteinization.

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“…Reports have attested that endocrine hormones play an important role in the development of ovaries [25]. Several Fox factors have been shown to be involved in regulating vitellogenesis, fecundity and ovarian events such as follicular development and selection, ovarian cell proliferation and cancerogenesis, ovarian cell apoptosis, ovarian secretory activity and oocyte/cumulus maturation [26,27]. However, whether FoxA influences reproduction or not is still unknown.…”

Section: Introductionmentioning

confidence: 99%

Fork head transcription factor is required for ovarian mature in the brown planthopper, Nilaparvata lugens (Stål)

et al. 2011

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BackgroundThe brown planthopper (BPH), Nilaparvata lugens, is the most devastating rice pest in many areas throughout Asia. The reproductive system of female N. lugens consists of a pair of ovaries with 24-33 ovarioles per ovary in most individuals which determine its fecundity. The fork head (Fox) is a transcriptional regulatory molecule, which regulates and controls many physiological processes in eukaryotes. The Fox family has several subclasses and members, and several Fox factors have been reported to be involved in regulating fecundity.ResultsWe have cloned a fork head gene in N. lugens. The full-length cDNA of NlFoxA is 1789 bp and has an open reading frame of 1143 bp, encoding a protein of 380 amino acids. Quantitative real-time PCR (RT-qPCR) and Reverse Transcription- PCR (RT-PCR) analysis revealed that NlFoxA mRNA was mainly expressed in the fat body, midgut, cuticle and Malpighian tube, and was expressed continuously with little change during all the developmental stages. NlFoxA belongs to the FoxA subfamily of the Fox transcription factors. Knockdown of NlFoxA expression by RNAi using artificial diet containing double-stranded RNA (dsRNA) significantly decreased the number of offspring and impacted the development of ovaries. ELISA and Western blot analyses showed that feeding-based RNAi of NlFoxA gene also resulted in decreased expression of vitellogenin (Vg) protein.ConclusionNlFoxA plays an important role in regulation of fecundity and development of ovaries in the BPH via regulating vitellogenin expression.

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Transcription factors and ovarian functions

Cited by 27 publications

References 57 publications

Changes in granulosa cells' gene expression associated with increased oocyte competence in bovine

Changes in granulosa cells' gene expression associated with increased oocyte competence in bovine

The role for runt related transcription factor 2 (RUNX2) as a transcriptional repressor in luteinizing granulosa cells

Fork head transcription factor is required for ovarian mature in the brown planthopper, Nilaparvata lugens (Stål)

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