Transcription factor SGF1 is critical for the neurodevelopment in the silkworm, Bombyx mori

Liu, Zhaoyang; Qi, Yu; Yang, Chunhong; Meng, Mingyao; Ren, Chunjiu; Mu, Zhimei; Cui, Wei-Zheng; Liu, Qingxin

doi:10.1016/j.gene.2016.04.034

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…Although RNAi success rate is low in several insect species, including silkworm, in the present study, application of RNAi efficiently suppressed silkworm U-shaped in larvae. Previously, this method has also been proved successful to knockdown partial genes, such as Vps4 (Xia et al, 2019), Kr-h1 (Zhu et al, 2020) and SGF1 (Liu et al, 2016). Recently, You et al (2020) improved the RNAi susceptibility of silkworm larvae by overexpressing RNAi's core machinery .…”

Section: Discussionmentioning

confidence: 99%

Bombyx mori U‐shaped regulates the melanization cascade and immune response via binding with the Lozenge protein

et al. 2021

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Zinc finger protein, an important transcription factor, regulates gene expression associated with various physiological and pathological processes. U‐shaped, belong to the Friend of GATA (FOG) transcription factor, plays a crucial role in hematopoiesis by interacting with the GATA transcription factor as a co‐factor. However, little is known about its functions in insects. In the present study, a U‐shaped cDNA was identified and characterized from the silkworm Bombyx mori and its potential roles in innate immunity investigated. The predicted silkworm U‐shaped amino acid sequence contained a classical nuclear localization signal (NLS) motif “GESSPKRRRR” at position 450–459, and arginine residues at position 456 and 478 are the critical sites of the NLS. U‐shaped mRNA was detected in all tested tissues of the B. mori; however, the highest levels were found in the hemocytes. U‐shaped mRNA expression levels were upregulated in the hemocyte after the Escherichia coli and Staphylococcus aureus challenge. Furthermore, U‐shaped knockdown significantly reduced the melanization process and suppressed the expression of melanization‐associated genes, including PPO1, PPO2, PPAE and BAEE. In addition, U‐shaped interacts with Lozenge protein to regulate the innate immune response of the insect. Our results revealed that U‐shaped binds directly to Lozenge protein to modulate the melanization process and innate immune responses in silkworm.

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Section: Discussionmentioning

confidence: 99%

Bombyx mori U‐shaped regulates the melanization cascade and immune response via binding with the Lozenge protein

et al. 2021

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“…The RNAi assay for BmFoxA was performed in Spli221 cells. A 355-bp fragment in the ORF region of BmFoxA was chosen as template for the synthesis of gene-specific dsRNA as described by Liu et al (25). Double-stranded RNA was synthesized from linearized templates by using the T7 RiboMAXTM Express RNAi system (Promega, Madison, WI).…”

Section: Rnaimentioning

confidence: 99%

“…Silk gland factor-1 (SGF-1) of B. mori is a homologue of D. melanogaster Fkh and plays a key role in the silk gland development by regulating the expression of ser-1, P25, fib-h, and other genes by binding to the Fox cis-regulatory element (CRE) (21)(22)(23)(24). SGF-1 is also important for the neurodevelopment (25). The specific binding between SGF-1 and the CRE in its target genes is influenced by the neighboring bases, and the efficient Fox elements may affect the promoter activity of the genes that express in the labial gland (26).…”

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confidence: 99%

Bombyx mori transcription factors FoxA and SAGE divergently regulate the expression of wing cuticle protein gene 4 during metamorphosis

Zhang

Zhao

et al. 2019

Journal of Biological Chemistry

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Edited by Xiao-Fan WangStage-specific gene expression governs metamorphosis of the silkworm, Bombyx mori. B. mori wing cuticle protein gene 4 (BmWCP4) is an essential gene for wing disc development expressed specifically during pupation. BmWCP4 transcription is suppressed at the larval stage by unknown mechanisms, which we sought to elucidate here. Bioinformatics analysis predicted seven potential Forkhead box (Fox) cis-regulatory elements (CREs) in the BmWCP4 promoter region, and we found that Fox CRE6 contributes to suppression of BmWCP4 expression. Electrophoretic mobility shift (EMSA) and DNA pull-down assays revealed that BmFoxA suppressed activity at the BmWCP4 promoter by specifically binding to the Fox CRE6. The expression level of BmFoxA in the wing discs was higher during the larval stage than at the pupal stage. In contrast, expression of another transcription factor, BmSAGE, increased over the course of development. Of note, the hormone 20-hydroxyecdysone (20E), which governs molting in insects, suppressed BmFoxA expression in the wing discs and up-regulated that of BmSage. EMSA and cell co-transfection assays indicated that BmSAGE interacted with BmFoxA and suppressed its binding to the Fox CRE6, thereby releasing BmFoxA-mediated suppression of BmWCP4. In summary, higher BmFoxA expression during the larval stage suppresses BmWCP4 expression by binding to the Fox CRE6 on the BmWCP4 promoter. During metamorphosis, BmSAGE forms a complex with BmFoxA to relieve this repression, initiating BmWCP4 expression. Taken together, this study reveals a switchlike role for BmFoxA in regulating BmWCP4 expression and provides new insights into the regulatory regulation of wing disc development in insects.

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Ras-Raf-MAPK signaling promotes nuclear localization of FOXA transcription factor SGF1 via Ser91 phosphorylation

Guo

et al. 2018

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Ras-Raf-MAPK signaling promotes cell proliferation and cell survival. We previously reported that Ras1 overexpression, specifically in the posterior silk glands (PSGs) of the silkworm Bombyx mori, increased fibroin synthesis and cell size, resulting in improved silk yields. In this study, we compared the iTRAQ-based phosphoproteomic profiles of PSGs from wild-type and Ras1-overexpressing silkworms. Silk gland factor 1 (SGF1), a FOXA transcription factor that plays a critical role in activating fibroin gene expression, was identified as a phosphoprotein harboring Ser91 as a potential MAPK phosphorylation site. Ser91 phosphorylation of SGF1 was enhanced by Ras1 overexpression, and this finding was verified by selected reaction monitoring. Consistently, MAPK activity is well correlated with Ser91 phosphorylation of SGF1 and its nuclear localization in PSG cells during silkworm development. Ras1 overexpression and treatment with inhibitors of Ras signaling promoted or inhibited SGF1 nuclear localization, respectively; mutation of Ser91 to Ala91 eliminated SGF1 nuclear localization. Moreover, MAPK binds to SGF1 and directly phosphorylates Ser91, demonstrating Ser91 as a MAPK phosphorylation site in SGF1. In conclusion, Ras-Raf-MAPK signaling promotes SGF1 nuclear localization for transactivation via Ser91 phosphorylation in silkworms, showing that FOXA transcription factors are regulated via MAPK phosphorylation in animals.

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Transcription factor SGF1 is critical for the neurodevelopment in the silkworm, Bombyx mori

Cited by 5 publications

References 31 publications

Bombyx mori U‐shaped regulates the melanization cascade and immune response via binding with the Lozenge protein

Bombyx mori U‐shaped regulates the melanization cascade and immune response via binding with the Lozenge protein

Bombyx mori transcription factors FoxA and SAGE divergently regulate the expression of wing cuticle protein gene 4 during metamorphosis

Ras-Raf-MAPK signaling promotes nuclear localization of FOXA transcription factor SGF1 via Ser91 phosphorylation

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