Transcription Factor OsWRKY53 Positively Regulates Brassinosteroid Signaling and Plant Architecture

Tian, Xiaojie; Li, Xiufeng; Zhou, Wenjia; Ren, Yuekun; Wang, Zhenyu; Liu, Zhiqi; Tang, Jufen; Tong, Hongning; Fang, Jun; Bu, Qingyun

doi:10.1104/pp.17.00946

Cited by 115 publications

(112 citation statements)

References 61 publications

(115 reference statements)

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“…OsWRKY6 influences resistance to Xanthomonas oryzae pv. oryzae by directly targeting and positively regulating OsWRKY6 gene expression (Choi et al, 2015), whereas OsWRKY53, an important regulator of brassinosteroid signaling, directly targets and negatively regulates OsWRKY53 expression (Tian et al, 2017). In pepper, CaWRKY22 is a positive regulator of resistance toward Ralstonia solanacearum (Hussain et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Principles and characteristics of the Arabidopsis WRKY regulatory network during early MAMP‐triggered immunity

et al. 2018

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During microbe-associated molecular pattern-triggered immunity more than 5000 Arabidopsis genes are significantly altered in their expression, and the question arises, how such an enormous reprogramming of the transcriptome can be regulated in a safe and robust manner? For the WRKY transcription factors (TFs), which are important regulators of numerous defense responses, it appears that they act in a complex regulatory sub-network rather than in a linear fashion, which would be much more vulnerable to gene function loss either by pathogen-derived effectors or by mutations. In this study we employed RNA-seq, mass spectrometry and chromatin immunoprecipitation-seq to find evidence for and uncover principles and characteristics of this network. Upon flg22-treatment, one can distinguish between two sets of WRKY genes: constitutively expressed and induced WRKY genes. Prior to elicitation the induced WRKY genes appear to be maintained in a repressed state mainly by the constitutively expressed WRKY factors, which themselves appear to be regulated by non-WRKY TFs. Upon elicitation, induced WRKYs rapidly bind to induced WRKY gene promoters and by auto- and cross-regulation build up the regulatory network. Maintenance of this flg22-induced network appears highly robust as removal of three key WRKY factors can be physically and functionally compensated for by other WRKY family members.

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Section: Introductionmentioning

confidence: 99%

Principles and characteristics of the Arabidopsis WRKY regulatory network during early MAMP‐triggered immunity

et al. 2018

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“…The transcript level of OsWRKY53 was greatly elevated in gla1 (Figure B). Coincidentally, overexpression of OsWRKY53 also caused an increase in grain size (Tian et al ). These data suggest that GLA1 may be located upstream of OsWRKY53 to negatively regulate the OsMAPKK4‐OsMAPK6‐OsWRKY53 module in rice grain size and BR signaling.…”

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confidence: 99%

“…OsMAPKK4 interacts with and phosphorylates its downstream OsMAPK6 to influence grain size by forming a signaling cascade (OsMAPKK4‐OsMAPK6) (Liu et al ). Besides, OsWRKY53 acts as a downstream effector of OsMAPKK4‐OsMAPK6 cascade to positively regulate rice grain size and BR signaling (Tian et al ), indicating that the OsMAPKK4‐OsMAPK6‐OsWRKY53 module is involved in grain size regulation and BR signaling pathways. However, the regulatory mechanism of the MAPK signaling modules controlling grain size remains largely unknown.…”

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confidence: 99%

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GRAIN LENGTH AND AWN 1 negatively regulates grain size in rice

Wang

Zou

et al. 2019

JIPB

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Summary Grain size is an important factor determining yield in rice. Here, we identified a recessive mutant gene, grain length and awn 1 (gla1), which caused a significant increase in grain length and weight, and was associated with long awns. The gla1 mutation was mapped to a single‐nucleotide polymorphism in a gene encoding a cytoplasmically‐localized mitogen‐activated protein kinase (MAPK) phosphatase. Overexpression of GLA1 caused a decrease in grain length, and the GLA1 protein interacted with OsMAPK6. These results suggest that GLA1 may serve as a negative regulator of the OsMAPKK4‐OsMAPK6 cascade, controlling grain size via the dephosphorylation of OsMAPK6.

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“…Accumulating evidence has revealed that WRKY proteins play diverse roles in responses to biotic and abiotic stresses and are involved in various processes of plant growth and development by regulating the expression of target genes via binding to the W-box cis-element (Rushton et al ., 2010). For example, OsWRKY53 overexpression led to enlarged leaf angles and increased grain size, in contrast to the erect leaves and smaller seeds in the oswrky53 mutant (Tian et al ., 2017). Based on our data, the mRNA levels of WRKY4 and WRKY72 were significantly higher in ZmCLA4-RNAi plants than in WT plants, indicating that ZmCLA4 directly repressed the expression of ABA-responsive WRKY4/72 (homologous gene of OsWRKY53) and mediated the ABA pathway to reduce leaf angle in maize.…”

Section: Discussionmentioning

confidence: 99%

ZmCLA4 regulates leaf angle through multiple plant hormone-mediated signal pathways in maize

Dou

Han

et al. 2020

Preprint

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Leaf angle in cereals is an important agronomic trait contributing to plant architecture and grain yield by determining the plant compactness. Although ZmCLA4 was identified to shape plant architecture by affecting leaf angle, the detailed regulatory mechanism of ZmCLA4 in maize remains unclear. ZmCLA4 was identified as a transcriptional repressor using the Gal4-LexA/UAS system and transactivation analysis in yeast. The DNA affinity purification (DAP)-seq assay showed that ZmCLA4 not only acts as a repressor containing the EAR motif (CACCGGAC), but was also found to have two new motifs, CCGARGS and CDTCNTC. On analyzing the ZmCLA4-bound targeted genes, we found that ZmCLA4, as a cross node of multiple plant hormone-mediated pathways, directly bound to ARF22 and IAA26 to regulate auxin transport and mediated brassinosteroid signaling by directly binding to BZR3 and 14-3-3. ZmCLA4 bound two WRKY genes involved with abscisic acid, two genes (CYP75B1, CYP93D1) involved with jasmonic acid, B3 involved in the response to ethylene, and thereby negatively regulated leaf angle formation. We built a new regulatory network for the ZmCLA4 gene controlling leaf angle in maize, which contributed to the understanding of ZmCLA4’s regulatory mechanism and will improve grain yields by facilitating optimization of plant architecture.

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Transcription Factor OsWRKY53 Positively Regulates Brassinosteroid Signaling and Plant Architecture

Cited by 115 publications

References 61 publications

Principles and characteristics of the Arabidopsis WRKY regulatory network during early MAMP‐triggered immunity

Principles and characteristics of the Arabidopsis WRKY regulatory network during early MAMP‐triggered immunity

GRAIN LENGTH AND AWN 1 negatively regulates grain size in rice

ZmCLA4 regulates leaf angle through multiple plant hormone-mediated signal pathways in maize

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