Transcription factor OCT4 promotes cell cycle progression by regulating CCND1 expression in esophageal carcinoma

Li, Zhigang; Li, Xinxin; Li, Chunguang; Su, Yinghan; Fang, Wentao; Zhong, Caiyun; Ji, Weidan; Zhang, Qian; Su, Changqing

doi:10.1016/j.canlet.2014.07.049

Cited by 31 publications

(23 citation statements)

References 26 publications

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“…The percentage of OCT4-positive cells was significantly increased in higher grade, higher non-classic differentiation number and more invasive urothelial bladder cancer cases (27). Oct4 was also suggested to promote proliferation and invasion of esophageal cancer cells (8). The data in the present study further supports this role for Oct4 in cancer progression.…”

Section: A B C D Discussionsupporting

confidence: 81%

“…Particularly, invasion is involved in cancer cell metastasis, which ultimately leads to a poor prognosis. The promotive effect of Oct4 on cancer cell proliferation was reported in certain types of cancer cells, including hepatocellular carcinoma (23) and esophageal cancer (8). The identification of Oct4-positive putative stem cells may result in uncontrolled cell proliferation and treatment failure in esophageal squamous cell carcinoma (24).…”

Section: A B C D Discussionmentioning

confidence: 99%

“…Oct4 has been identified as overexpressed in human embryonic carcinoma (5), prostate cancer (6), and bladder cancer cells, and been associated with enhanced migration and invasion (7). Oct4 was also demonstrated to promote cell cycle progression in esophageal carcinoma (8), promote colony formation of glioma cells (9), and increase transmigration capacity in melanoma cells (10). These previous studies suggest that deregulation and dysfunction of Oct4 contribute to malignant establishment of a cancer stem cell phenotype (5).…”

Section: Introductionmentioning

confidence: 92%

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Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

et al. 2016

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Abstract. Osteosarcoma is the most common primary bone tumor in children and adolescents, typically presenting with a poor prognosis. Octamer-binding transcription factor 4 (Oct4) protein, encoded by the POU class 5 homeobox 1 gene, is important in maintaining self-renewal of pluripotent stem cells, and is closely associated with cancer. However, its role in osteosarcoma remains to be elucidated. The present study observed Oct4 was markedly increased in osteosarcoma cell lines and in human osteosarcoma tissue samples. Following Oct4 downregulation by small interfering RNA (siRNA) in osteosarcoma F5M2 cells, the cells exhibited significant decreases in proliferation and invasion ability, and an increase in cell apoptosis. Notably, downregulation of Oct4 decreased the expression of AK055347, a newly identified long noncoding RNA (lncRNA) in human tissues. The downregulation of AK055347 by siRNA resulted in a significant suppressive effect on proliferative and invasive ability, and promotion of cell apoptosis in osteosarcoma cells. Thus, the current study suggests Oct4 exerts a promoting effect in osteosarcoma, and identifies a novel lncRNA in osteosarcoma progression.

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Section: A B C D Discussionsupporting

confidence: 81%

Section: A B C D Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

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Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

et al. 2016

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“…Overall, miR-4458 may participate in inhibiting the onset of lung cancer, as a cancer suppressor gene. CCND1 protein is encoded by the human CCND1 gene (36). CCND1 is important in controlling the cell growth cycle, and numerous types of cancer abnormally express CCND1 proteins at a high level to stimulate cell growth (37).…”

Section: Discussionmentioning

confidence: 99%

Role of microRNA-4458 in patients with non-small-cell lung cancer

et al. 2016

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Abstract. Incidence and progression of non-small-cell lung cancer (NSCLC) is a multi-factor, multi-step process. The present study investigated the association between the expression level of microRNA (miR)-4458 in NSCLC and paracarcinoma liver tissues and survival rates, and studied the biological functions of miR-4458 at the cellular and protein level. NSCLC and paracarcinoma tissues were sequenced using a miR expression chip. The association between miR-4458 expression and tumor-node-metastasis staging, total survival rate and relapse-free survival rate was analyzed. miR-4458 was subjected to target gene prediction. The target protein of cyclin D1 (CCND1) was verified with western blot analysis, immunohistochemistry and a luciferase reporter assay. The relative level of miR-4458 in paracarcinoma tissues of 9 NSCLC patients decreased from 2.38 to 0.65 (P<0.001). Total five-year survival rates of the high-expression miR-4458 group (29.21%) significantly exceeded that of the low-expression group (14.37%) (P=0.025). The viability of human lung carcinoma A549 and H460 cells transfected with miR-4458 decreased significantly compared with cells transfected with a normal control (blank control plasmid) within 72 h (P<0.001). The percentage of A549 and H460 cells transfected with a miR-4458 mimic at the cell cycle stage G0/G1 was 69.94±8.05 and 68.15±7.75%, respectively. The percentages increased significantly compared with the control group (46.06±6.93 for A549 cells; 45.22±7.24 for H640 cells; P<0.001). CCND1 mRNA was downregulated significantly in H460 cells 72 h subsequent to the addition of miR-4458 mimics (P<0.001). The activity of mutant-CCND1 altered slightly, while the fluorescence intensity of the wild-type-CCND1 group decreased significantly following the addition of miR-4458 mimics. In conclusion, miR-4458 was expressed at low levels in lung cancer tissues, and it arrested cells in vitro at stage G0/G1 and inhibited cell proliferation. Therefore, miR-4458 may participate in the onset of lung cancer as a suppressor gene by inhibiting CCND1.

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“…Li et al reported that OCT4 is capable of repressing Tgf β 3 and Tgf β R3 and downregulating the epithelial-to-mesenchymal transition (EMT) regulator Snail (13). In esophageal carcinoma, OCT4 regulates CCND1 expression to promote cell progression and accelerate cell proliferation and invasion (14). Atlasi et al reported that the long variant OCT4A was found to be required for the pluripotency properties of ES cells (11).…”

Section: Introductionmentioning

confidence: 99%

OCT4B1 promotes cell growth, migration and invasion suppressing sensitivity to οxaliplatin in colon cancer

et al. 2015

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Abstract. OCT4B1, a splice variant of OCT4, is a key regulator in maintaining the properties of pluripotency and self-renewal in embryonic stem (ES) cells. Recent results have shown that OCT4B1 is involved in tumorigenesis. However, the contribution of OCT4B1 in the tumorigenesis and drug resistance of colon cancer remains to be determined. The aim of the present study was to determine whether OCT4B1, which maintains the stemness of ES cells, promoted cell growth by facilitating transition of the cell cycle and reduced apoptosis in colon cancer and drug-resistant cells using flow cytometry and western blotting. The results showed that, OCT4B1 promoted the growth of colon cancer and drug-resistant cancer cells by maintaining the activity of ES cells and by facilitating the transition of the cell cycle and reducing apoptosis. Additionally, OCT4B1 was able to reduce sensitivity to oxaliplatin by altering the expression of two important mediators in drug resistance, P-gp and ABCG2 [ATP-binding cassette, sub-family G (WHITE), member 2]. Furthermore, OCT4B1 enhanced the ability of migration and invasion through alteration of the epithelial-to-mesenchymal transition (EMT) in colon cancer. In conclusion, to the best of our knowledge, the results demonstrated for the first time that OCT4B1 functions as an oncogene in colon cancer and provides the development of novel therapeutic strategies to treat colon cancer, particularly drug resistance.

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Transcription factor OCT4 promotes cell cycle progression by regulating CCND1 expression in esophageal carcinoma

Cited by 31 publications

References 26 publications

Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

Role of microRNA-4458 in patients with non-small-cell lung cancer

OCT4B1 promotes cell growth, migration and invasion suppressing sensitivity to οxaliplatin in colon cancer

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