Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes

Abstract

Transcription factor Lbx1 is known to play a role in the migration of muscle progenitor cells in limb buds and also in neuronal determination processes. In addition, involvement of Lbx1 in cardiac neural crest-related cardiogenesis was postulated. Here, we used mouse embryonic stem (ES) cells which have the capacity to develop into cells of all three primary germ layers. During in vitro differentiation, ES cells recapitulate cellular developmental processes and gene expression patterns of early embryogenesis. Transcript analysis revealed a significant upregulation of Lbx1 at the progenitor cell stage. Immunofluorescence staining confirmed the expression of Lbx1 in skeletal muscle cell progenitors and GABAergic neurons. To verify the presence of Lbx1 in cardiac cells, triple immunocytochemistry of ES cell-derived cardiomyocytes and a quantification assay were performed at different developmental stages. Colabeling of Lbx1 and cardiac specific markers troponin T, α-actinin, GATA4, and Nkx2.5 suggested a potential role in early myocardial development.

Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation

“…The ladybird protein is a member of the homeobox transcription factor family with an engrailed repressor domain [ 22 ]. In vertebrates, Lbx genes are expressed in the dorsal spinal cord and hindbrain [ 23 ], a subpopulation of cardiac neural crest cells [ 24 ], muscle precursor cells, and satellite cells of regenerating adult skeletal muscle [ 25 , 26 ]. Ectopic expression of LBX1 in chicken somites and limbs activates myogenic markers such as myogenin and myod , owing to the expansion of the myoblastic cell population [ 27 ].…”

The Susceptibility and Potential Functions of the LBX1 Gene in Adolescent Idiopathic Scoliosis

“…The LBX1 gene plays an essential role in regulating muscle precursor cell migration and maintaining its migratory potential ( Figure 1 ) (Brohmann et al, 2000 ). Previous transcript analyses and immunofluorescence staining confirmed the upregulation and location of LBX1 in muscle precursor cells (Schmitteckert et al, 2011 ). Migratory muscle precursor cells could remain undifferentiated during migration, and LBX1+ cells commence differentiation into skeletal muscle cells after arrival in the limb (Tani-Matsuhana et al, 2018 ).…”

Study on the Developmental Expression ofLbx1Gene inLongissimus Dorsiof Mashen and Large White Pigs

“…Muscle hypertrophy depended on the increasing of myofibrils and of the nucleus which relayed on fusion of satellite cells (Campion et al, 1981). Functionally, Lbx1 could induce myogenesis through guiding muscle precursor migration to the proper regions (Schafer and Braun 1999;Schmitteckert et al, 2011) and controlling myoblasts proliferation (Mennerich and Braun, 2001;Martin and Harland, 2006). Furthermore, Lbx1 played important roles in satellite cells differentiation and self-renew (Watanabe et al, 2007).…”

“…During myogenesis in mammals and amphibians, Lbx1 was necessary for the the migration of muscle precursor cells (Jagla et al, 1997;Schafer and Braun, 1999;Gross, et al, 2002;Schafer et al, 2003;Cheng et al, 2005;Martin and Harland, 2006;Wotton et al, 2008;Schmitteckert et al, 2011;). Previous studies have demonstrated that Lbx1 and Pax3 were co-expressed in all migration hypaxial muscle precursors (Gross, et al, 2000).…”