Transcription factor <i>AtMYB103</i> is required for anther development by regulating tapetum development, callose dissolution and exine formation in Arabidopsis

Zhang, Zaibao; Zhu, Jun; Gao, Jufang; Wang, Chen; Li, Hui; Li, Hong; Hui-qi, Zhang; Zhang, Sen; Wang, Dong-Mi; Wang, Quanxi; Huang, Hai; Xia, Hui-Jun; Yang, Zhong‐Nan

doi:10.1111/j.1365-313x.2007.03254.x

Cited by 336 publications

(320 citation statements)

References 37 publications

(57 reference statements)

Supporting

Mentioning

313

Contrasting

Order By: Relevance

“…Premature or retarded tapetal degeneration frequently causes male sterility (N. Li et al, 2007;Zhang et al, 2007;Shi et al, 2009;Phan et al, 2011). Genetic investigations discovered several transcription factors involved in rice tapetal degeneration, such as GAMYB (Kaneko et al, 2004;Liu et al, 2010), TDR (N. , EAT1 (Niu et al, 2013), and Os-API5 (X.…”

Section: Tip2 Regulates Tapetal Pcdmentioning

confidence: 99%

The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development

et al. 2014

View full text Add to dashboard Cite

In male reproductive development in plants, meristemoid precursor cells possessing transient, stem cell-like features undergo cell divisions and differentiation to produce the anther, the male reproductive organ. The anther contains centrally positioned microsporocytes surrounded by four distinct layers of wall: the epidermis, endothecium, middle layer, and tapetum. Here, we report that the rice (Oryza sativa) basic helix-loop-helix (bHLH) protein TDR INTERACTING PROTEIN2 (TIP2) functions as a crucial switch in the meristemoid transition and differentiation during early anther development. The tip2 mutants display undifferentiated inner three anther wall layers and abort tapetal programmed cell death, causing complete male sterility. TIP2 has two paralogs in rice, TDR and EAT1, which are key regulators of tapetal programmed cell death. We revealed that TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1. In addition, TIP2 can interact with TDR, indicating a role of TIP2 in later anther development. Our findings suggest that the bHLH proteins TIP2, TDR, and EAT1 play a central role in regulating differentiation, morphogenesis, and degradation of anther somatic cell layers, highlighting the role of paralogous bHLH proteins in regulating distinct steps of plant cell-type determination.

show abstract

Section: Tip2 Regulates Tapetal Pcdmentioning

confidence: 99%

The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The callase mixture includes endoglucanases and exoglucanases (Stieglitz and Stern, 1973;Scott et al, 2004). Endoglucanases cleave b-1,3-glucans into short-chain reducing sugars; exoglucanase hydrolysis releases a single Glc unit from the reducing ends of the substrate (Stieglitz, 1977;Zhang et al, 2007). A6 was suggested to possess endoglucanase activity (Hird et al, 1993).…”

Section: Callose Dissolution During Pollen Development Is a Highly Comentioning

confidence: 99%

“…It was known that callose defects can affect pollen wall formation and pollen viability (Dong et al, 2005;Enns et al, 2005;Zhang et al, 2007). Thus, we examined the expression of MALE STERILITY2 (MS2), DEFEC-TIVE IN EXINE FORMATION1 (DEX1), NO EXINE FORMATION1 (NEF1), and FACELESS POLLEN1 (FLP1), which were involved in pollen exine formation and sporopollenin synthesis (Aarts et al, 1997;PaxsonSowders et al, 2001;Ariizumi et al, 2003Ariizumi et al, , 2004, in both the wild type and cdm1 by qRT-PCR.…”

Section: The Expression Of Pollen Developmental Genes Was Changed In mentioning

confidence: 99%

The Arabidopsis CALLOSE DEFECTIVE MICROSPORE1 Gene Is Required for Male Fertility through Regulating Callose Metabolism during Microsporogenesis

Chai

Yang

et al. 2014

Plant Physiol.

View full text Add to dashboard Cite

ORCID IDs: 0000-0002-3941-9955 (P.L.); 0000-0001-8717-4422 (H.M.).During angiosperm microsporogenesis, callose serves as a temporary wall to separate microsporocytes and newly formed microspores in the tetrad. Abnormal callose deposition and dissolution can lead to degeneration of developing microspores. However, genes and their regulation in callose metabolism during microsporogenesis still remain largely unclear. Here, we demonstrated that the Arabidopsis (Arabidopsis thaliana) CALLOSE DEFECTIVE MICROSPORE1 (CDM1) gene, encoding a tandem CCCH-type zinc finger protein, plays an important role in regulation of callose metabolism in male meiocytes and in integrity of newly formed microspores. First, quantitative reverse transcription PCR and in situ hybridization analyses showed that the CDM1 gene was highly expressed in meiocytes and the tapetum from anther stages 4 to 7. In addition, a transfer DNA insertional cdm1 mutant was completely male sterile. Moreover, light microscopy of anther sections revealed that microspores in the mutant anther were initiated, and then degenerated soon afterward with callose deposition defects, eventually leading to male sterility. Furthermore, transmission electron microscopy demonstrated that pollen exine formation was severely affected in the cdm1 mutant. Finally, we found that the cdm1 mutation affected the expression of callose synthesis genes (CALLOSE SYNTHASE5 and CALLOSE SYNTHASE12) and potential callase-related genes (A6 and MYB80), as well as three other putative b-1,3-glucanase genes. Therefore, we propose that the CDM1 gene regulates callose metabolism during microsporogenesis, thereby promoting Arabidopsis male fertility.

show abstract

“…We identified 1,569 down-regulated genes and 848 upregulated genes (2,417 in total) with a change in expression of 2-fold or more in the tapetum and/or microspores at stages 8 and 9 between the wild type and ptc1 (Supplemental Data Set S1). Some genes previously identified as critical for postmeiotic anther development were down-regulated in the ptc1 microarray data (Table I), including a Cys protease (OsCP1; Lee et al, 2004), a fatty acyl-CoA reductase homologous to Arabidopsis MS2 (Aarts et al, 1997), lipid transfer proteins (Osc4, OsC6, YY1, and Os09g0525500; Tsuchiya et al, 1992;Hihara et al, 1996), a stilbene synthase (YY2; Hihara et al, 1996), BURP domain-containing proteins (RA8 and OsRAFTIN; Jeon et al, 1999), a P450 family member (CYP704B2; , a ribosomeinactivating protein (RA39; Jeon et al, 1999), and the putative homolog of AtMYB103 (Higginson et al, 2003;Zhang et al, 2007). Consistent with the RT-qPCR analysis, PTC1 showed up-regulation in the ptc1 mutant (2.97-fold at stage 8, 5.49-fold at stage 9), whereas genes involved in early tapetal and pollen regulation, such as CYP703A3 (Aya et al, 2009), TDR (Li et al, 2006), UDT1 (Jung et al, 2005), and GAMYB (Kaneko et al, 2004;Liu et al, 2010; Table I), showed no significant change.…”

Section: Ptc1 Is Preferentially Expressed In the Tapetum And Microspomentioning

confidence: 99%

PERSISTENT TAPETAL CELL1Encodes a PHD-Finger Protein That Is Required for Tapetal Cell Death and Pollen Development in Rice

et al. 2011

Self Cite

View full text Add to dashboard Cite

In higher plants, timely degradation of tapetal cells, the innermost sporophytic cells of the anther wall layer, is a prerequisite for the development of viable pollen grains. However, relatively little is known about the mechanism underlying programmed tapetal cell development and degradation. Here, we report a key regulator in monocot rice (Oryza sativa), PERSISTANT TAPETAL CELL1 (PTC1), which controls programmed tapetal development and functional pollen formation. The evolutionary significance of PTC1 was revealed by partial genetic complementation of the homologous mutation MALE STERILITY1 (MS1) in the dicot Arabidopsis (Arabidopsis thaliana). PTC1 encodes a PHD-finger (for plant homeodomain) protein, which is expressed specifically in tapetal cells and microspores during anther development in stages 8 and 9, when the wild-type tapetal cells initiate a typical apoptosis-like cell death. Even though ptc1 mutants show phenotypic similarity to ms1 in a lack of tapetal DNA fragmentation, delayed tapetal degeneration, as well as abnormal pollen wall formation and aborted microspore development, the ptc1 mutant displays a previously unreported phenotype of uncontrolled tapetal proliferation and subsequent commencement of necrosis-like tapetal death. Microarray analysis indicated that 2,417 tapetum-and microspore-expressed genes, which are principally associated with tapetal development, degeneration, and pollen wall formation, had changed expression in ptc1 anthers. Moreover, the regulatory role of PTC1 in anther development was revealed by comparison with MS1 and other rice anther developmental regulators. These findings suggest a diversified and conserved switch of PTC1/MS1 in regulating programmed male reproductive development in both dicots and monocots, which provides new insights in plant anther development.

show abstract

Transcription factor AtMYB103 is required for anther development by regulating tapetum development, callose dissolution and exine formation in Arabidopsis

Cited by 336 publications

References 37 publications

The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development

The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development

The Arabidopsis CALLOSE DEFECTIVE MICROSPORE1 Gene Is Required for Male Fertility through Regulating Callose Metabolism during Microsporogenesis

PERSISTENT TAPETAL CELL1Encodes a PHD-Finger Protein That Is Required for Tapetal Cell Death and Pollen Development in Rice

Contact Info

Product

Resources

About