Transcription factor EB protects against endoplasmic reticulum stress in human coronary artery endothelial cells

Haas, Michael J.; Feng, Victoria; Gonzales, Krista; Bikkina, Priyanka; Landicho, Marie Angelica; Mooradian, Arshag D.

doi:10.1016/j.ejphar.2022.175274

Cited by 8 publications

(2 citation statements)

References 31 publications

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“…Lysosomal stress can also activate transcription factor EB (TFEB), which acts as a main regulator of lysosomal biogenesis and function [55]. TFEB is regulated by multiple signaling pathways and can modulate several processes that are important in atherosclerosis, including lipophagy, autophagy, lipolysis, and inflammation [56][57][58][59][60][61]. Thus, lysosomal biogenesis in macrophages stimulated by TFEB may serve as a protective factor for atherosclerosis [62].…”

Section: Lysosomal Dysfunction In Atherosclerosismentioning

confidence: 99%

The Role of Punicalagin and Its Metabolites in Atherosclerosis and Risk Factors Associated with the Disease

Alalawi,

Albalawi,

Ramji

2023

IJMS

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Atherosclerotic cardiovascular disease (ACVD) is the leading cause of death worldwide. Although current therapies, such as statins, have led to a marked reduction in morbidity and mortality from ACVD, they are associated with considerable residual risk for the disease together with various adverse side effects. Natural compounds are generally well-tolerated; a major recent goal has been to harness their full potential in the prevention and treatment of ACVD, either alone or together with existing pharmacotherapies. Punicalagin (PC) is the main polyphenol present in pomegranates and pomegranate juice and demonstrates many beneficial actions, including anti-inflammatory, antioxidant, and anti-atherogenic properties. The objective of this review is to inform on our current understanding of the pathogenesis of ACVD and the potential mechanisms underlying the beneficial actions of PC and its metabolites in the disease, including the attenuation of dyslipidemia, oxidative stress, endothelial cell dysfunction, foam cell formation, and inflammation mediated by cytokines and immune cells together with the regulation of proliferation and migration of vascular smooth muscle cells. Some of the anti-inflammatory and antioxidant properties of PC and its metabolites are due to their strong radical-scavenging activities. PC and its metabolites also inhibit the risk factors of atherosclerosis, including hyperlipidemia, diabetes mellitus, inflammation, hypertension, obesity, and non-alcoholic fatty liver disease. Despite the promising findings that have emerged from numerous in vitro, in vivo, and clinical studies, deeper mechanistic insights and large clinical trials are required to harness the full potential of PC and its metabolites in the prevention and treatment of ACVD.

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Section: Lysosomal Dysfunction In Atherosclerosismentioning

confidence: 99%

The Role of Punicalagin and Its Metabolites in Atherosclerosis and Risk Factors Associated with the Disease

Alalawi,

Albalawi,

Ramji

2023

IJMS

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“…Te ER stress is mediated by three unfolded protein response (UPR) mediators [10][11][12][13]. Inositol requiring enzyme 1α (IRE1α), protein kinase RNA-like endoplasmic reticulum kinase (PERK), and activating transcription factor 6 (ATF6) are all localized to the ER membrane and associate with glucose regulated protein 78 (GRP78), an ER-resident molecular chaperone [10].…”

Section: Introductionmentioning

confidence: 99%

Omega-3 Fatty Acids Inhibit Endoplasmic Reticulum (ER) Stress in Human Coronary Artery Endothelial Cells

Warda,

Graham,

Batch-Joudi

et al. 2023

Journal of Food Biochemistry

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The biological effects of fatty acids differ by their structure. Saturated fatty acids and trans-fatty acids are recognized as promoters of coronary artery disease (CAD), while monounsaturated and omega-3 fatty acids may have salutary effects. Since cellular stress is recognized as a fundamental driver of CAD, the effect of these fatty acids on endoplasmic reticulum (ER) stress in human coronary artery endothelial cells (HCAECs) was measured using the ER stress-responsive alkaline phosphatase (ES-TRAP) assay. Docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and eicosapentaenoic acid ethyl ester (EPA-EE) suppressed ER stress induced with pharmacologic (tunicamycin) and physiologic (high-dextrose concentration) ER stress inducers. In tunicamycin-treated cells, DHA reduced the expression of unfolded protein response (UPR) markers such as phosphorylation of inositol requiring enzyme 1α (IRE1α) and protein kinase R-like endoplasmic reticulum kinase (PERK) and increased activating transcription factor 6 (ATF6) and glucose regulated protein 78 (GRP78) expression. Similarly, treatment with both oleic acid and arachidonic acid, but not elaidic acid (a trans-fatty acid), suppressed both tunicamycin and high-dextrose-induced ER stress while treatment with saturated fatty acids (C14 : 0, C16 : 0, and C18 : 0) enhanced both tunicamycin and high-dextrose-induced ER stress. The latter fatty acids at higher concentrations caused cytotoxicity. These results indicate that omega-3 fatty acids as well as select unsaturated fatty acids and arachidonic acid suppress ER stress in HCAEC.

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Cardiomodulatory Effects of Cardiometabolic and Antihyperglycemic Medications: The Roles of Oxidative and Endoplasmic Reticulum Stress

Mooradian

2024

Am J Cardiovasc Drugs

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Transcription factor EB protects against endoplasmic reticulum stress in human coronary artery endothelial cells

Cited by 8 publications

References 31 publications

The Role of Punicalagin and Its Metabolites in Atherosclerosis and Risk Factors Associated with the Disease

The Role of Punicalagin and Its Metabolites in Atherosclerosis and Risk Factors Associated with the Disease

Omega-3 Fatty Acids Inhibit Endoplasmic Reticulum (ER) Stress in Human Coronary Artery Endothelial Cells

Cardiomodulatory Effects of Cardiometabolic and Antihyperglycemic Medications: The Roles of Oxidative and Endoplasmic Reticulum Stress

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