Transcription Factor CBF4 Is a Regulator of Drought Adaptation in Arabidopsis

Haake, Volker; Cook, Daniel; Riechmann, José Luis; Pineda, Omaira; Thomashow, Michael F.; Zhang, James Z.

doi:10.1104/pp.006478

Cited by 659 publications

(551 citation statements)

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“…To date, a number of transcription factor genes from different families such as DREB (Liu et al 1998;Kasuga et al 1999;Yamaguchi-Shinozaki and Shinozaki 2001;Haake et al 2002), MYB (Abe et al 1997), bZIP (Uno et al 2000), zinc finger (Mukhopadhyay et al 2004) and NAC Tran et al 2004) has been reported as having effect on improving stress tolerance. However, the evidence of improved stress resistance for most of these genes is from model species such as Arabidopsis.…”

Section: Discussionmentioning

confidence: 99%

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

et al. 2008

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Plants respond to adverse environment by initiating a series of signaling processes including activation of transcription factors that can regulate expression of arrays of genes for stress response and adaptation. NAC (NAM, ATAF, and CUC) is a plant specific transcription factor family with diverse roles in development and stress regulation. In this report, a stress-responsive NAC gene (SNAC2) isolated from upland rice IRA109 (Oryza sativa L. ssp japonica) was characterized for its role in stress tolerance. SNAC2 was proven to have transactivation and DNA-binding activities in yeast and the SNAC2-GFP fusion protein was localized in the rice nuclei. Northern blot and SNAC2 promoter activity analyses suggest that SNAC2 gene was induced by drought, salinity, cold, wounding, and abscisic acid (ABA) treatment. The SNAC2 gene was over-expressed in japonica rice Zhonghua 11 to test the effect on improving stress tolerance. More than 50% of the transgenic plants remained vigorous when all WT plants died after severe cold stress (4-8°C for 5 days). The transgenic plants had higher cell membrane stability than wild type during the cold stress. The transgenic rice had significantly higher germination and growth rate than WT under high salinity conditions. Over-expression of SNAC2 can also improve the tolerance to PEG treatment. In addition, the SNAC2-overexpressing plants showed significantly increased sensitivity to ABA. DNA chip profiling analysis of transgenic plants revealed many up-regulated genes related to stress response and adaptation such as peroxidase, ornithine aminotransferase, heavy metal-associated protein, sodium/hydrogen exchanger, heat shock protein, GDSL-like lipase, and phenylalanine ammonia lyase. Interestingly, none of the up-regulated genes in the SNAC2-overexpressing plants matched the genes up-regulated in the transgenic plants over-expressing other stress responsive NAC genes reported previously. These data suggest SNAC2 is a novel stress responsive NAC transcription factor that possesses potential utility in improving stress tolerance of rice.

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Section: Discussionmentioning

confidence: 99%

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

et al. 2008

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“…The protein sequences of all GmCBF-like/DREB1 genes are most similar to that of AtCBF4 (AtDREB1d) sequence (Figure 1). AtCBF4 is thought to be derived from same ancestor as AtCBF1-3 based on coding sequence and promoter sequence similarity between AtCBF4 and AtCBF1-3 [14]. There is a greater degree of synteny in the neighboring genes to GmDREB1A and 1B genes to the region surrounding AtCBF4 than to the region surrounding the tandem-linked AtCBF1-3 (data not shown); suggesting that the GmCBF/DREB1 genes may be derived more recently from the same ancestor as AtCBF4.…”

Section: Comparison Of Cold-inducible Cbf/dreb1 Genes In Soybean Withmentioning

confidence: 97%

“…AtCBF1, AtCBF2, and AtCBF3 (DREB1B, DREB1C, and DREB1A; respectively) are up regulated significantly and rapidly by cold stress specifically, but much less so by ABA or dehydration [12,13]. In contrast, AtCBF4 (DREB1D) and AtDREB2 genes are more specifically regulated by ABA, salinity, and dehydration but not by cold stress [12,14,15].…”

Section: Introductionmentioning

confidence: 99%

“…Overexpression of AtDREB1A or AtDREB2A in Arabidopsis transgenic plants induces the expression of cold responsive genes such as RD29a and COR47 without exposure to low temperature [18][19][20]. The accumulation of downstream genes mediated by CBF/DREB1 genes, increases abiotic stress tolerances to freezing, dehydration, and high salt [14,[20][21][22][23][24]. In cold-intolerant tomato, the transgenic expression of AtCBF1 gene increased cold regulated genes [25] [ 26,27] and the expression of AtCBF1 in tomato increases freezing tolerance [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

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Functionality of soybean CBF/DREB1 transcription factors

Yamasaki

Randall

2016

Plant Science

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Soybean (Glycine max) is considered to be cold intolerant and is not able to significantly acclimate to cold/freezing stress. In most cold tolerant plants, the Crepeat/DRE Binding Factors (CBF/DREBs) are critical contributors to successful cold-responses; rapidly increasing following cold treatment and regulating the induction of many cold responsive genes. In soybean vegetative tissue, we found strong, transient accumulation of CBF transcripts in response to cold stress; however, the soybean transcripts of typical cold responsive genes (homologues to Arabidopsis genes such as dehydrins, ADH1, RAP2.1, and LEA14) were not significantly altered.Soybean CBFs were found to be functional, as when expressed constitutively in Arabidopsis they increased the levels of AtCOR47 and AtRD29a transcripts and increased freezing tolerance as measured by a decrease in leaf freezing damage and ion leakage. Furthermore the constitutive expression of GmDREB1A;2 and GmDREB1B;1 in Arabidopsis led to stronger up-regulation of downstream genes and more freezing tolerance than GmDREB1A;1, the gene whose transcript is the major contributor to total CBF/DREB1 transcripts in soybean. The inability for the soybean CBFs to significantly up regulate the soybean genes that contribute to cold tolerance is consistent with poor acclimation capability and the cold intolerance of soybean.

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“…Arabidopsis RGLG2 is a RING domain E3 ligase which mediates ubiquitination of the transcription factor Ethylene Response Factor 53 (ERF53) for proteasome degradation (Cheng et al 2012). The expression of some droughtresponsive transcription factors genes such as DREB2A, CBF4 and RAP2.4 is controlled by ERF53 (Haake et al 2002;Lin et al 2008;Sakuma et al 2006). Therefore, downregulation of RGLG2 expression is required for induction of ERF53 and thereby activation of its downstream genes under drought stress.…”

Section: Stress Signaling Through Ros Regulationmentioning

confidence: 99%

Mining expressed sequence tags of rapeseed (Brassica napus L.) to predict the drought responsive regulatory network

Shamloo‐Dashtpagerdi

Razi

Ebrahimie

2015

Physiol Mol Biol Plants

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It is of great significance to understand the regulatory mechanisms by which plants deal with drought stress. Two EST libraries derived from rapeseed (Brassica napus) leaves in non-stressed and drought stress conditions were analyzed in order to obtain the transcriptomic landscape of drought-exposed B. napus plants, and also to identify and characterize significant drought responsive regulatory genes and microRNAs. The functional ontology analysis revealed a substantial shift in the B. napus transcriptome to govern cellular drought responsiveness via different stress-activated mechanisms. The activity of transcription factor and protein kinase modules generally increased in response to drought stress. The 26 regulatory genes consisting of 17 transcription factor genes, eight protein kinase genes and one protein phosphatase gene were identified showing significant alterations in their expressions in response to drought stress. We also found the six microRNAs which were differentially expressed during drought stress supporting the involvement of a posttranscriptional level of regulation for B. napus drought response. The drought responsive regulatory network shed light on the significance of some regulatory components involved in biosynthesis and signaling of various plant hormones (abscisic acid, auxin and brassinosteroids), ubiquitin proteasome system, and signaling through Reactive Oxygen Species (ROS). Our findings suggested a complex and multi-level regulatory system modulating response to drought stress in B. napus.

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Transcription Factor CBF4 Is a Regulator of Drought Adaptation in Arabidopsis

Cited by 659 publications

References 52 publications

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

Functionality of soybean CBF/DREB1 transcription factors

Mining expressed sequence tags of rapeseed (Brassica napus L.) to predict the drought responsive regulatory network

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