RETRACTED ARTICLE: Transcription elongation factor A-like 7, regulated by miR-758-3p inhibits the progression of melanoma through decreasing the expression levels of c-Myc and AKT1

Li, Xilin; Song, Xianji; Li, Hong

doi:10.1186/s12935-020-01737-3

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…Reported roles for miR-758-3p include the regulation of proliferation, migration and invasion of carcinoma cells (Wu & Liu 2020, Liu et al 2021, Xiao et al 2021) via modulation of c-Myc and AKT signalling and long noncoding (lincRNA) and circular RNA (circRNA) actions (Ding et al 2021, Xiao et al 2021, Zhang et al 2021. While miR-758-3p do not influence proliferation in the placenta, migration and invasion are also key features of normal placentation (Knofler & Pollheimer 2012), and key roles for lincRNA and circRNA in placental and fetal development are emerging (Gong et al 2021).…”

Section: Discussionmentioning

confidence: 99%

miR-514a-3p: a novel SHP-2 regulatory miRNA that modulates human cytotrophoblast proliferation

Quilang,

Lui,

Forbes

2023

EJEA

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Src homology-2 domain-containing protein tyrosine phosphatase 2 (SHP-2), encoded by the PTPN11 gene, forms a central component of multiple signalling pathways and is required for insulin-like growth factor (IGF)-induced placental growth. Altered expression of SHP-2 is associated with aberrant placental and fetal growth indicating that drugs modulating SHP-2 expression may improve adverse pregnancy outcome associated with altered placental growth. We have previously demonstrated that placental PTPN11/SHP-2 expression is controlled by miRNAs. SHP-2 regulatory miRNAs may have therapeutic potential; however, the individual miRNA(s) that regulate SHP-2 expression in the placenta remain to be established. We performed in silico analysis of 3'UTR target prediction databases to identify libraries of Hela cells transfected with individual miRNA mimetics, enriched in potential SHP-2 regulatory miRNAs. Analysis of PTPN11 levels by quantitative (q) PCR revealed that miR-758-3p increased, while miR-514a-3p reduced PTPN11 expression. The expression of miR-514a-3p and miR-758-3p within the human placenta was confirmed by qPCR; miR-514a-3p (but not miR-758-3p) levels inversely correlated with PTPN11 expression. To assess the interaction between these miRNAs and PTPN11/ SHP-2, specific mimetics were transfected into first-trimester human placental explants and then cultured for up to 4 days. Overexpression of miR-514a-3p, but not miR-758-3p, significantly reduced PTPN11 and SHP-2 expression. microRNA-ribonucleoprotein complex (miRNP)-associated mRNA assays confirmed that this interaction was direct. miR-514a-3p overexpression attenuated IGF-I-induced trophoblast proliferation (BrdU incorporation). miR-758-3p did not alter trophoblast proliferation. These data demonstrate that by modulating SHP-2 expression, miR-514a-3p is a novel regulator of IGF signalling and proliferation in the human placenta and may have therapeutic potential in pregnancies complicated by altered placental growth.

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Section: Discussionmentioning

confidence: 99%

miR-514a-3p: a novel SHP-2 regulatory miRNA that modulates human cytotrophoblast proliferation

Quilang,

Lui,

Forbes

2023

EJEA

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“…More experiments are required to examine this hypothesis. Recently, a number of miRNAs have been reported in cancer progression through targeting human Tceal7, such as miR-182, miR-301a, miR-769-5p, miR-18b and miR-758-3p [57][58][59][60][61]. Taken together, more research will be required in the future to understand the complicated regulatory network of the Tceal7 gene.…”

Section: Discussionmentioning

confidence: 99%

Transcription Regulation of Tceal7 by the Triple Complex of Mef2c, Creb1 and Myod

Xiong

Wang

You

et al. 2022

Biology

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Tceal7 has been identified as a direct, downstream target gene of MRF in the skeletal muscle. The overexpression of Tceal7 represses myogenic proliferation and promotes cell differentiation. Previous studies have defined the 0.7 kb upstream fragment of the Tceal7 gene. In the present study, we have further determined two clusters of transcription factor-binding motifs in the 0.7 kb promoter: CRE#2–E#1–CRE#1 in the proximal region and Mef2#3–CRE#3–E#4 in the distal region. Utilizing transcription assays, we have also shown that the reporter containing the Mef2#3–CRE#3–E#4 motifs is synergistically transactivated by Mef2c and Creb1. Further studies have mapped out the protein–protein interaction between Mef2c and Creb1. In summary, our present studies support the notion that the triple complex of Mef2c, Creb1 and Myod interacts with the Mef2#3–CRE#3–E#4 motifs in the distal region of the Tceal7 promoter, thereby driving Tceal7 expression during skeletal muscle development and regeneration.

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“…Reported roles for miR-758-3p include regulation of proliferation, migration and invasion of carcinoma cells (Wu and Liu, 2020, Xiao et al, 2021, Liu et al, 2021 via modulation of c-Myc and AKT signalling and long-non-coding (lincRNA) and circular RNA (circRNA)…”

Section: Discussionmentioning

confidence: 99%

miR-514a-3p: a novel SHP-2 regulatory miRNA that modulates human cytotrophoblast proliferation

Quilang

Liu

Forbes

2022

Journal of Molecular Endocrinology

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Src homology-2 (SH2) domain containing protein tyrosine phosphatase 2 (SHP-2), encoded by the PTPN11 gene, forms a central component of multiple signalling pathways and is required for insulin-like growth factor (IGF) induced placental growth. Altered expression of SHP-2 is associated with aberrant placental and fetal growth indicating that drugs modulating SHP-2 expression may improve adverse pregnancy outcome associated with altered placental growth. Placental PTPN11/SHP-2 expression is controlled by microRNAs (miRNAs) so SHP-2 regulatory miRNAs may have therapeutic potential, however the individual miRNA(s) regulating placental SHP-2 expression remain to be established. We performed in-silico analysis of 3’UTR target prediction databases to identify libraries of HeLa cells transfected with individual miRNA-mimetics, enriched in potential SHP-2 regulatory miRNAs. Analysis of PTPN11 levels by qPCR revealed that miR-758-3p increased, whilst miR-514a-3p reduced PTPN11 expression. miR-514a-3p and miR-758-3p expression within the human placenta was confirmed by qPCR; miR-514a-3p (but not miR-758-3p) levels inversely correlated with PTPN11 expression. To assess the interaction between these miRNAs and PTPN11/SHP-2, specific mimetics were transfected into first trimester human placental explants and explants cultured for up to 4 days. Overexpression of miR-514a-3p, but not miR-758-3p, significantly reduced PTPN11 and SHP-2 expression. microRNA-ribonucleoprotein complex (miRNP)-associated mRNA assays confirmed that this interaction was direct. miR-514a-3p overexpression attenuated IGF-I induced trophoblast proliferation (BrdU incorporation). miR-758-3p did not alter trophoblast proliferation. These data demonstrate that by modulating SHP-2 expression, miR-514a-3p is a novel regulator of IGF- signalling and proliferation in the human placenta and may have therapeutic potential in pregnancies complicated by altered placental growth.

show abstract

RETRACTED ARTICLE: Transcription elongation factor A-like 7, regulated by miR-758-3p inhibits the progression of melanoma through decreasing the expression levels of c-Myc and AKT1

Cited by 5 publications

References 33 publications

miR-514a-3p: a novel SHP-2 regulatory miRNA that modulates human cytotrophoblast proliferation

miR-514a-3p: a novel SHP-2 regulatory miRNA that modulates human cytotrophoblast proliferation

Transcription Regulation of Tceal7 by the Triple Complex of Mef2c, Creb1 and Myod

miR-514a-3p: a novel SHP-2 regulatory miRNA that modulates human cytotrophoblast proliferation

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