1984
DOI: 10.1128/mcb.4.7.1293
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Transcription control region within the protein-coding portion of adenovirus E1A genes.

Abstract: A single-base deletion within the protein-coding region of the adenovirus type 5 early region lA (EIA) genes, 399 bases downstream from the transcription start site, depresses transcription to 2% of the wild-type rate. Complementation studies demonstrated that this was due to two effects of the mutation: first, inactivation of an ElA protein, causing a reduction by a factor of 5; second, a defect which acts in cis to depress EIA mRNA and nuclear RNA concentrations by a factor of 10. A larger deletion within th… Show more

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Cited by 59 publications
(30 citation statements)
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“…Although the regulatory role(s) that factors Bi and B2, which bind to intragenic sites, may have in the control of P-globin expression has not been determined, examples exist of intragenic regulatory elements in the immunoglobulin genes (3,21) and the adenovirus ElA gene (35). The possible roles for factors Bi and B2 include: (i) negative control of P-globin expression in the uninduced MEL cell; (ii) a necessary, but not sufficient, positive role involving establishment of a permissive conformation on the P-globin gene in the uninduced MEL cell; and (iii) a positive requirement for 3-globin expression upon the induction of the MEL cell.…”
Section: Resultsmentioning
confidence: 99%
“…Although the regulatory role(s) that factors Bi and B2, which bind to intragenic sites, may have in the control of P-globin expression has not been determined, examples exist of intragenic regulatory elements in the immunoglobulin genes (3,21) and the adenovirus ElA gene (35). The possible roles for factors Bi and B2 include: (i) negative control of P-globin expression in the uninduced MEL cell; (ii) a necessary, but not sufficient, positive role involving establishment of a permissive conformation on the P-globin gene in the uninduced MEL cell; and (iii) a positive requirement for 3-globin expression upon the induction of the MEL cell.…”
Section: Resultsmentioning
confidence: 99%
“…However, the locations of these enhancers were initially regarded as a peculiarity of the immunoglobulin gene loci. More recently, internal control regions have been detected (but yet undefined) for the adenovirus ElA gene (38), human globin genes (3), and chicken thymidine kinase gene (31). Any conclusion that the conserved ,-actin intron sequences, especially those of IVS I, function as transcriptional enhancers must await direct experimentation.…”
Section: Methodsmentioning
confidence: 99%
“…ElA proteins are known to have positive transcriptional effects on different viral and cellular genes in the following cases: early viral genes on the viral genome, including the ElA gene itself (3,19,22,30,31); early viral genes on cellular chromosomes (8); activation of the 3-globin gene on a transfected circular plasmid (16, 34); and activation of chromosomal heat shock (23) and P-tubulin genes (33). In addition, ElA proteins can cause a suppressing effect on transfected enhancer-dependent transcriptional units in the circular simian virus 40 genome (4,35).…”
mentioning
confidence: 99%