Transcription and splicing: A two‐way street

Abstract

Previous research has suggested that individuals with Autism Spectrum Disorders (ASD) have difficulties understanding others communicative intent and with using contextual information to correctly interpret irony. We recorded the eye movements of typically developing (TD) adults ASD adults when they read statements that could either be interpreted as ironic or non-ironic depending on the context of the passage. Participants with ASD performed as well as TD controls in their comprehension accuracy for speaker's statements in both ironic and non-ironic conditions. Eye movement data showed that for both participant groups, total reading times were longer for the critical region containing the speaker's statement and a subsequent sentence restating the context in the ironic condition compared to the non-ironic condition. The results suggest that more effortful processing is required in both ASD and TD participants for ironic compared with literal non-ironic statements, and that individuals with ASD were able to use contextual information to infer a non-literal interpretation of ironic text. Individuals with ASD however spent more time overall than TD controls re-reading the passages, to a similar degree across both ironic and non-ironic conditions, suggesting that they either take longer to construct a coherent discourse representation of the text, or that they take longer to make the decision that their representation of the text is reasonable based on their knowledge of the world.

Widespread Transcriptional Readthrough Caused by Nab2 Depletion Leads to Chimeric Transcripts with Retained Introns

“…When protein-coding genes are transcribed by RNA polymerase II (RNA Pol II), the nascent RNA undergoes 5 0 end capping, splicing within the transcript body, and 3 0 end cleavage before a mature mRNA can be exported to the cytoplasm for translation. The majority of these processing events are co-transcriptional and closely coordinated with transcription and chromatin regulation (Alpert et al, 2017;Saldi et al, 2016;Tellier et al, 2020). For example, cleavage at the 3 0 end initiates transcription termination.…”

The Alter Retina: Alternative Splicing of Retinal Genes in Health and Disease

“…Moreover, chromatin organization can also change or determine splicing patterns. The spliceosome catalyzes splicing in a two-step trans-esterification reaction that joins each donor site to the correspondent acceptor site [ 8 ].…”

Transcriptional kinetic synergy: a complex landscape revealed by integrating modelling and synthetic biology

“…In addition, RNA polymerase has been found to be already bound on many inactive genes, suggesting that under certain scenarios activation does not rely on regulating polymerase binding, but modulating a subsequent step (Oven et al, 2007). Furthermore, the splicing machinery can also affect transcription (Tellier et al, 2020).…”