Transcription and reverse transcription of artificial nucleic acids involving backbone modification by template-directed DNA polymerase reactions

Kuwahara, Masayasu; Takeshima, Hidetoshi; Nagashima, Jun-ichi; Minezaki, Satoshi; Ozaki, Hiroaki; Sawai, Hiroaki

doi:10.1016/j.bmc.2009.04.045

Cited by 16 publications

(12 citation statements)

References 43 publications

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“… 27 For these reasons artificial DNA backbones which are less challenging to form chemically are an attractive alternative. 28 Prevalent amongst these are the triazole, 29 amide 30 , 31 and phosphoramidate 32 – 34 linkages ( Fig. 1(ii) ).…”

Section: Introductionmentioning

confidence: 99%

Enzyme-free synthesis of cyclic single-stranded DNA constructs containing a single triazole, amide or phosphoramidate backbone linkage and their use as templates for rolling circle amplification and nanoflower formation

et al. 2018

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Section: Introductionmentioning

confidence: 99%

Enzyme-free synthesis of cyclic single-stranded DNA constructs containing a single triazole, amide or phosphoramidate backbone linkage and their use as templates for rolling circle amplification and nanoflower formation

et al. 2018

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“…3). Certain thermophilic and mutant polymerases such as Pwo, Vent (exo-), Deep Vent (exo-), and KOD Dash have been actively developed for incorporation of modified nucleotides during polymerization (Gudima et al ., 1998; Kuwahara et al ., 2003; Leal et al ., 2006; Kuwahara et al ., 2009; Veedu et al ., 2009; Kuwahara et al ., 2010).…”

Section: Stabilization Of the Oligonucleotide Aptamermentioning

confidence: 99%

Nucleic Acid Aptamers: New Methods for Selection, Stabilization, and Application in Biomedical Science

Kong¹,

Byun²

2013

Biomolecules and Therapeutics

158

115

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The adoption of oligonucleotide aptamer is well on the rise, serving an ever increasing demand for versatility in biomedical field. Through the SELEX (Systematic Evolution of Ligands by EXponential enrichment), aptamer that can bind to specific target with high affinity and specificity can be obtained. Aptamers are single-stranded nucleic acid molecules that can fold into complex threedimensional structures, forming binding pockets and clefts for the specific recognition and tight binding of any given molecular target. Recently, aptamers have attracted much attention because they not only have all of the advantages of antibodies, but also have unique merits such as thermal stability, ease of synthesis, reversibility, and little immunogenicity. The advent of novel technologies is revolutionizing aptamer applications. Aptamers can be easily modified by various chemical reactions to introduce functional groups and/or nucleotide extensions. They can also be conjugated to therapeutic molecules such as drugs, drug containing carriers, toxins, or photosensitizers. Here, we discuss new SELEX strategies and stabilization methods as well as applications in drug delivery and molecular imaging.

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“…However, the primary reason to incorporate the acetamido group next to the phosphate or diphosphate is a replacement of the ionic phosphate by the neutral amide bond, which may be considered as a non‐charged phosphate bioisostere . Furthermore, the replacement of the phosphate internucleotide bonds with an acetamido linkage has also been investigated . Although the phosphate and carbamoyl groups differ in length, flexibility, polarity, lipophilicity, and hydrogen bonding capabilities, increased stability toward enzymatic hydrolysis in comparison to phosphates makes this replacement valuable and thus it deserves further studies.…”

Section: Introductionmentioning

confidence: 99%

Novel Acyclic Phosphonylated 1,2,3‐Triazolonucleosides with an Acetamidomethyl Linker: Synthesis and Biological Activity

Głowacka

Balzarini

Wróblewski

2014

Archiv der Pharmazie

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A new series of 4-substituted [(1,2,3-triazol-1-yl)acetamido]methylphosphonates as acyclic nucleotide analogs were synthesized from diethyl (2-chloroacetamido)methylphosphonate via azidation followed by 1,3-dipolar cycloaddition with selected alkynes derived from natural nucleobases or their mimetics. All compounds were tested for their antiviral activities against DNA and RNA viruses as well as for cytostatic activity or cytotoxicity. Among all tested compounds, [(1,2,3-triazol-1-yl)acetamido]methylphosphonate 6e substituted with the N(3)-Bz-benzuracil moiety showed activity against the vesicular stomatitis virus (EC50 = 45 µM) in HeLa cell cultures.

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Transcription and reverse transcription of artificial nucleic acids involving backbone modification by template-directed DNA polymerase reactions

Cited by 16 publications

References 43 publications

Enzyme-free synthesis of cyclic single-stranded DNA constructs containing a single triazole, amide or phosphoramidate backbone linkage and their use as templates for rolling circle amplification and nanoflower formation

Enzyme-free synthesis of cyclic single-stranded DNA constructs containing a single triazole, amide or phosphoramidate backbone linkage and their use as templates for rolling circle amplification and nanoflower formation

Nucleic Acid Aptamers: New Methods for Selection, Stabilization, and Application in Biomedical Science

Novel Acyclic Phosphonylated 1,2,3‐Triazolonucleosides with an Acetamidomethyl Linker: Synthesis and Biological Activity

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