Transcription and Post-translational Regulation of Autophagy in Insects

Li, Rongsong; Xiao, Yang; Li, Kang; Tian, Ling

doi:10.3389/fphys.2022.825202

Cited by 6 publications

(4 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Likewise, the negative correlation of F3, composed of hymenoptaecin , an antimicrobial peptide gene (Casteels et al, 1993), and Cyp6a14.1 , a putative detoxification enzyme gene (Mao et al, 2009), with DWV suggests a complex response to the viral pathogen, and, potentially, virally mediated suppression of important host functions. It is, however, notable that none of the genes examined in the present study are known to be associated with cellular autophagy (Li et al, 2022), which is the immune pathway shown to be affected by exposure to Sylgard‐309 (Aronstein & Saldivar, 2005; Nakamoto et al, 2012). In the future, the effects of TSS adjuvants on this pathway in adults should be further examined to better explain the treatment‐dependent increases in DWV abundance reported in our study.…”

Section: Discussionmentioning

confidence: 67%

Trisiloxane Surfactants Negatively Affect Reproductive Behaviors and Enhance Viral Replication in Honey Bees

Fine,

Cox‐Foster,

Moor

et al. 2023

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Trisiloxane surfactants are often applied in formulated adjuvant products to blooming crops including almonds, exposing the managed honey bees (Apis mellifera) used for pollination of these crops and persisting in colony matrices like bee bread. Despite this, little is known regarding the effects of trisiloxane surfactants on important aspects of colony health like reproduction. Here, we use laboratory assays to examine how exposure to field relevant concentrations of three trisiloxane surfactants found in commonly used adjuvant formulations affect queen oviposition rates, worker interactions with the queen, and worker susceptibility to endogenous viral pathogens. Trisiloxane surfactants were administered at 5 mg/kg in pollen supplement diet for 14 days. No effects on worker behavior or physiology could be detected, but our results demonstrate that hydroxy‐capped trisiloxane surfactants can negatively affect queen oviposition and methyl capped trisiloxane surfactants cause increased replication of Deformed Wing Virus in workers, suggesting that trisiloxane surfactant use while honey bees are foraging may negatively impact colony longevity and growth.

show abstract

Section: Discussionmentioning

confidence: 67%

Trisiloxane Surfactants Negatively Affect Reproductive Behaviors and Enhance Viral Replication in Honey Bees

Fine,

Cox‐Foster,

Moor

et al. 2023

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…This complex responds effectively to reduced insulin signaling, extending lifespan, and highlighting the significance of autophagy in insect longevity. Notably, the phosphorylation of Atg1 by AMPK, especially at Ser269 and Ser270, is essential for autophagy initiation in both species (Li et al, 2022;Zhao et al, 2023). Phosphorylation/dephosphorylation pathways in eukaryotes.…”

Section: Phosphorylation and Dephosphorylationmentioning

confidence: 99%

Protein modification regulated autophagy in Bombyx mori and Drosophila melanogaster

Wu,

Lin,

Zhao

et al. 2023

Front. Physiol.

View full text Add to dashboard Cite

Post-translational modifications refer to the chemical alterations of proteins following their biosynthesis, leading to changes in protein properties. These modifications, which encompass acetylation, phosphorylation, methylation, SUMOylation, ubiquitination, and others, are pivotal in a myriad of cellular functions. Macroautophagy, also known as autophagy, is a major degradation of intracellular components to cope with stress conditions and strictly regulated by nutrient depletion, insulin signaling, and energy production in mammals. Intriguingly, in insects, 20-hydroxyecdysone signaling predominantly stimulates the expression of most autophagy-related genes while concurrently inhibiting mTOR activity, thereby initiating autophagy. In this review, we will outline post-translational modification-regulated autophagy in insects, including Bombyx mori and Drosophila melanogaster, in brief. A more profound understanding of the biological significance of post-translational modifications in autophagy machinery not only unveils novel opportunities for autophagy intervention strategies but also illuminates their potential roles in development, cell differentiation, and the process of learning and memory processes in both insects and mammals.

show abstract

“…From a regulatory perspective, in D. melanogaster, autophagy induction is under the control of the target of rapamycin (TOR) pathway [24,[38][39][40][41]. TOR inhibition in response to stress or exposure to rapamycin causes a cellular increase in autophagic flux, i.e.…”

Section: Introductionmentioning

confidence: 99%

Exploring the connection between autophagy and heat-stress tolerance in Drosophila melanogaster

Willot,

du Toit,

de Wet

et al. 2023

Proc. R. Soc. B.

View full text Add to dashboard Cite

Mechanisms aimed at recovering from heat-induced damages are closely associated with the ability of ectotherms to survive exposure to stressful temperatures. Autophagy, a ubiquitous stress-responsive catabolic process, has recently gained renewed attention as one of these mechanisms. By increasing the turnover of cellular structures as well as the clearance of long-lived protein and protein aggregates, the induction of autophagy has been linked to increased tolerance to a range of abiotic stressors in diverse ectothermic organisms. However, whether a link between autophagy and heat-tolerance exists in insect models remains unclear despite broad ecophysiological implications thereof. Here, we explored the putative association between autophagy and heat-tolerance using Drosophila melanogaster as a model. We hypothesized that (i) heat-stress would cause an increase of autophagy in flies' tissues, and (ii) rapamycin exposure would trigger a detectable autophagic response in adults and increase their heat-tolerance. In line with our hypothesis, we report that flies exposed to heat-stress present signs of protein aggregation and appear to trigger an autophagy-related homoeostatic response as a result. We further show that rapamycin feeding causes the systemic effect associated with target of rapamycin (TOR) inhibition, induces autophagy locally in the fly gut, and increases the heat-stress tolerance of individuals. These results argue in favour of a substantial contribution of autophagy to the heat-stress tolerance mechanisms of insects.

show abstract

Transcription and Post-translational Regulation of Autophagy in Insects

Cited by 6 publications

References 75 publications

Trisiloxane Surfactants Negatively Affect Reproductive Behaviors and Enhance Viral Replication in Honey Bees

Trisiloxane Surfactants Negatively Affect Reproductive Behaviors and Enhance Viral Replication in Honey Bees

Protein modification regulated autophagy in Bombyx mori and Drosophila melanogaster

Exploring the connection between autophagy and heat-stress tolerance in Drosophila melanogaster

Contact Info

Product

Resources

About