Transcript profiling reveals potential regulators for oxidative stress response of a necrotrophic chickpea pathogen Ascochyta rabiei

Maurya, Ranjeet; Singh, Yeshveer; Sinha, Michael S.; Singh, K. B.; Mishra, Pallavi; Singh, Sudha; Verma, Sandhya; Prabha, K. Rama; Kumar, Kamal; Verma, Praveen Kumar

doi:10.1007/s13205-020-2107-8

Cited by 14 publications

(6 citation statements)

References 42 publications

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“…Stressful environmental conditions trigger excessive production of reactive oxygen species (ROS), such as superoxide radical (O 2− ), hydrogen peroxide H 2 O 2 , hydroxyl radicals (OH), and singlet oxygen (O 2 ; Maurya et al, 2020 ; Turan, 2020 ; Rehman et al, 2021b ). According to Asada and Takahashi (1987) , 1 % of oxygen is diverted to produce ROS in plants.…”

Section: Introductionmentioning

confidence: 99%

Effects of Light Spectra on Morphology, Gaseous Exchange, and Antioxidant Capacity of Industrial Hemp

et al. 2022

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One of the most important growth factors in cannabis cultivation is light which plays a big role in its successful growth. However, understanding that how light controls the industrial hemp growth and development is poor and needs advanced research. Therefore, a pot study was conducted to investigate the effects of different colors of light, that is, white light (WL), blue light (BL), red light (RL), and 50% red with 50% blue mix light (RBL) on morphology, gaseous exchange and antioxidant capacity of industrial hemp. Compared with WL, BL significantly increase hemp growth in terms of shoot fresh biomass (15.1%), shoot dry biomass (27.0%), number of leaves per plant (13.7%), stem diameter (10.2%), root length (6.8%) and chlorophyll content (7.4%). In addition, BL promoted net photosynthesis, stomatal conductance, and transpiration, while reduces the lipid peroxidation and superoxide dismutase and peroxidase activities. However, RL and RBL significantly reduced the plant biomass, gas exchange parameters with enhanced antioxidant enzymes activities. Thus, blue light is useful for large-scale sustainable production of industrial hemp.

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Section: Introductionmentioning

confidence: 99%

Effects of Light Spectra on Morphology, Gaseous Exchange, and Antioxidant Capacity of Industrial Hemp

et al. 2022

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“…The oxidative stress response of pathogens to external stimuli is generally used to understand their acclimatization mechanisms ( Maurya et al, 2020 ). In the present study, intracellular ROS and MDA production were increased ( Figure 6 ), verifying that ROS and MDA-mediated oxidative stress played a significant role in the antimicrobial effect of moringin against L. monocytogenes .…”

Section: Discussionmentioning

confidence: 99%

Multi-Target Antibacterial Mechanism of Moringin From Moringa oleifera Seeds Against Listeria monocytogenes

Wen

et al. 2022

Front. Microbiol.

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Moringin [4-(α-L-rhamnosyloxy) benzyl isothiocyanate] is an isothiocyanate from Moringa oleifera seeds. It is the bioactivated form of the glucosinolate precursor glucomoringin with various health benefits. However, few studies have examined the antibacterial activity of moringin. This study aimed to investigate the antimicrobial activity and mechanism of moringin against Listeria monocytogenes. The minimum inhibitory concentration (MIC), and growth curves were used to evaluate the bacteriostatic effect of moringin against L. monocytogenes. Transcriptome analysis by RNA sequencing was performed to elucidate the underlying mechanism of moringin against L. monocytogenes. The transcriptome results were validated. The results showed that moringin inhibited the growth of L. monocytogenes with a MIC of 400 μM. RNA sequencing results showed that the differences in the expression of genes related to the cell wall and membrane biosynthesis, phosphotransferase system (PTS), oxidative stress, energy metabolism, and DNA binding were significantly affected. As with the transcriptome results, the results of the mechanism verification found that moringin damaged the integrity of the cell wall and cell membrane, stimulated oxidative stress, interfered with energy metabolism and DNA replication, and finally led to the death of L. monocytogenes. The present study provides evidence that moringin exhibits strong antimicrobial activity against L. monocytogenes and insight into its potential mechanism.

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“…Recently, by using a reference‐based transcriptomics approach, 73 differentially expressed genes were identified under oxidative stress conditions (Maurya et al, 2020 ). These findings constitute the beginning of functional genomics of A. rabiei that will help in the detailed investigation of transcriptional reprogramming during infection.…”

Section: Molecular Studiesmentioning

confidence: 99%

Ascochyta rabiei: A threat to global chickpea production

Singh

Kumar

Purayannur

et al. 2022

Molecular Plant Pathology

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The necrotrophic fungus Ascochyta rabiei causes Ascochyta blight (AB) disease in chickpea. A. rabiei infects all aerial parts of the plant, which results in severe yield loss. At present, AB disease occurs in most chickpea‐growing countries. Globally increased incidences of A. rabiei infection and the emergence of new aggressive isolates directed the interest of researchers toward understanding the evolution of pathogenic determinants in this fungus. In this review, we summarize the molecular and genetic studies of the pathogen along with approaches that are helping in combating the disease. Possible areas of future research are also suggested. Taxonomy kingdom Mycota, phylum Ascomycota, class Dothideomycetes, subclass Coelomycetes, order Pleosporales, family Didymellaceae, genus Ascochyta , species rabiei. Primary host A. rabiei survives primarily on Cicer species. Disease symptoms A. rabiei infects aboveground parts of the plant including leaves, petioles, stems, pods, and seeds. The disease symptoms first appear as watersoaked lesions on the leaves and stems, which turn brown or dark brown. Early symptoms include small circular necrotic lesions visible on the leaves and oval brown lesions on the stem. At later stages of infection, the lesions may girdle the stem and the region above the girdle falls off. The disease severity increases at the reproductive stage and rounded lesions with concentric rings, due to asexual structures called pycnidia, appear on leaves, stems, and pods. The infected pod becomes blighted and often results in shrivelled and infected seeds. Disease management strategies Crop failures may be avoided by judicious practices of integrated disease management based on the use of resistant or tolerant cultivars and growing chickpea in areas where conditions are least favourable for AB disease development. Use of healthy seeds free of A. rabiei , seed treatments with fungicides, and proper destruction of diseased stubbles can also reduce the fungal inoculum load. Crop rotation with nonhost crops is critical for controlling the disease. Planting moderately resistant cultivars and prudent application of fungicides is also a way to combat AB disease. However, the scarcity of AB‐resistant accessions and the continuous evolution of the pathogen challenges the disease management process. Useful websites https://www.ndsu.edu/pubweb/pulse‐info/resourcespdf/Ascochyta%20blight%20of%20chickpea.pdf https://saskpulse.com/files/newsletters/180531_ascochyta_in_chickpeas‐compressed.pdf http://www.pulseaus.com.au/growing‐pulses/bmp/chickpea/ascochyta‐blight http://agriculture.vic.gov.au/agriculture/pests‐diseases...

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Transcript profiling reveals potential regulators for oxidative stress response of a necrotrophic chickpea pathogen Ascochyta rabiei

Cited by 14 publications

References 42 publications

Effects of Light Spectra on Morphology, Gaseous Exchange, and Antioxidant Capacity of Industrial Hemp

Effects of Light Spectra on Morphology, Gaseous Exchange, and Antioxidant Capacity of Industrial Hemp

Multi-Target Antibacterial Mechanism of Moringin From Moringa oleifera Seeds Against Listeria monocytogenes

Ascochyta rabiei: A threat to global chickpea production

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