Transcript profiling leads to biomarker identification for agarwood resin-loaded Aquilaria malaccensis

Islam, Md. Rofiqul; Banu, Sofia

doi:10.1007/s00468-021-02180-1

Cited by 5 publications

(3 citation statements)

References 45 publications

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“…In the course of this study, we employed an in silico approach, unveiling the differential upregulation of genes associated with the MAPK signaling pathway, transcription factors, and the biosynthesis of terpene backbones. The same had also been validated and is in align with our few previous studies where we obtained differential regulation of genes encoding MAPK, WRKY, MYC2, and terpenoid biosynthesis through qRT-PCR in naturally infected wood (Islam et al, 2020;Das et al, 2021;Islam and Banu, 2021;Das et al, 2023). We have previously observed that naturally infected Aquilaria woods exhibited higher expression levels of the genes responsible for sesquiterpene biosynthesis, which include ADXPS, AHMGR, AFPS, ASS, DGS, and ADXPR (Islam et al, 2020).…”

Section: Discussionsupporting

confidence: 87%

“…We have previously observed that naturally infected Aquilaria woods exhibited higher expression levels of the genes responsible for sesquiterpene biosynthesis, which include ADXPS, AHMGR, AFPS, ASS, DGS, and ADXPR (Islam et al, 2020). In the same way, in the infected agarwood, a higher expression of signaling genes (MK, WRKY1, and MAPK3), jasmonate biosynthesis genes (MYC2 and LOX), and sesquiterpenes genes (DSS, DGS, DXPS, FPS, SS4, and DGS1) was observed (Islam and Banu, 2021). An in silico investigation shows that the molecular mechanism behind the production of numerous types of aromatic chemicals is attributed by a AaTPS gene family (Das et al, 2021).…”

Section: Discussionmentioning

confidence: 62%

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Genome-wide analysis of respiratory burst oxidase homolog (Rboh) genes in Aquilaria species and insight into ROS-mediated metabolites biosynthesis and resin deposition

Begum,

Das,

Ahmed

et al. 2024

Front. Plant Sci.

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Respiratory burst oxidase homolog (Rboh) generates reactive oxygen species (ROS) as a defense response during biotic and abiotic stress. In Aquilaria plants, wounding and fungal infection result in biosynthesis and deposition of secondary metabolites as defense responses, which later form constituents of fragrant resinous agarwood. During injury and fungal invasion, Aquilaria tree generates ROS species via the Rboh enzymes. Despite the implication of Rboh genes in agarwood formation, no comprehensive genomic-level study of the Rboh gene family in Aquilaria is present. A systematic illustration of their role during stress and involvement in initiating signal cascades for agarwood metabolite biosynthesis is missing. In this study, 14 Rboh genes were retrieved from genomes of two Aquilaria species, A. agallocha and A. sinensis, and were classified into five groups. The promoter regions of the genes had abundant of stress-responsive elements. Protein–protein network and in silico expression analysis suggested their functional association with MAPK proteins and transcription factors such as WRKY and MYC2. The study further explored the expression profiles of Rboh genes and found them to be differentially regulated in stress-induced callus and stem tissue, suggesting their involvement in ROS generation during stress in Aquilaria. Overall, the study provides in-depth insight into two Rboh genes, AaRbohC and AaRbohA, highlighting their role in defense against fungal and abiotic stress, and likely during initiation of agarwood formation through modulation of genes involved in secondary metabolites biosynthesis. The findings presented here offer valuable information about Rboh family members, which can be leveraged for further investigations into ROS-mediated regulation of agarwood formation in Aquilaria species.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 62%

Genome-wide analysis of respiratory burst oxidase homolog (Rboh) genes in Aquilaria species and insight into ROS-mediated metabolites biosynthesis and resin deposition

Begum,

Das,

Ahmed

et al. 2024

Front. Plant Sci.

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“…The mechanism of Qi-Nan agarwood formation is still not fully comprehended, but it is quite clear that the primary components of agarwood from Qi-Nan are sesquiterpenes and phenylethyl chromones ( Islam and Banu, 2021 ), and the principal sesquiterpenes constituents of A. sinensis agarwood include guaianes, eudesmanes, acoranes, eremophilanes, cadinanes, agarospiranes, zizaanes, humulanes and prezizaanes. These compounds are primarily found in plants, predominantly as volatile components within essential oils, and are characterized by distinct aromas that enhance the fragrance of agarwood ( Li et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Anatomical, chemical and endophytic fungal diversity of a Qi-Nan clone of Aquilaria sinensis (Lour.) Spreng with different induction times

Li,

Fang,

Cui

et al. 2024

Front. Plant Sci.

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Recently, some new Qi-Nan clones of Aquilaria sinensis (Lour.) Spreng which intensively produces high-quality agarwood have been identified and propagated through grafting techniques. Previous studies have primarily focused on ordinary A. sinensis and the differences in composition when compared to Qi-Nan and ordinary A. sinensis. There are few studies on the formation mechanism of Qi-Nan agarwood and the dynamic changes in components and endophytic fungi during the induction process. In this paper, the characteristics, chemical composition, and changes in endophytic fungi of Qi-Nan agarwood induced after 1 year, 2 years, and 3 years were studied, and Qi-Nan white wood was used as the control. The results showed that the yield of Qi-Nan agarwood continued to increase with the induction time over a period of 3 years, while the content of alcohol extract from Qi-Nan agarwood reached its peak at two years. During the formation of agarwood, starch and soluble sugars in xylem rays and interxylary phloem are consumed and reduced. Most of the oily substances in agarwood were filled in xylem ray cells and interxylary phloem, and a small amount was filled in xylem vessels. The main components of Qi-Nan agarwood are also chromones and sesquiterpenes. With an increasing induction time, the content of sesquiterpenes increased, while the content of chromones decreased. The most abundant chromones in Qi-Nan agarwood were 2-(2-Phenethyl) chromone, 2-[2-(3-Methoxy-4-hydroxyphenyl) ethyl] chromone, and2-[2-(4-Methoxyphenyl) ethyl] chromone. Significant differences were observed in the species of the endophytic fungi found in Qi-Nan agarwood at different induction times. A total of 4 phyla, 73 orders, and 448 genera were found in Qi-Nan agarwood dominated by Ascomycota and Basidiomycota. Different induction times had a significant effect on the diversity of the endophytic fungal community in Qi-Nan. After the induction of agarwood formation, the diversity of Qi-Nan endophytic fungi decreased. Correlation analysis showed that there was a significant positive correlation between endophytic fungi and the yield, alcohol extract content, sesquiterpene content, and chromone content of Qi-Nan agarwood, which indicated that endophytic fungi play a role in promoting the formation of Qi-Nan agarwood. Qi-Nan agarwood produced at different induction times exhibited strong antioxidant capacity. DPPH free radical scavenging activity and reactive oxygen species clearance activity were significantly positively correlated with the content of sesquiterpenes and chromones in Qi-Nan agarwood.

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Genome-wide investigation of Cytochrome P450 superfamily of Aquilaria agallocha: Association with terpenoids and phenylpropanoids biosynthesis

Das

Begum

Akhtar

et al. 2023

International Journal of Biological Macromolecules

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Transcript profiling leads to biomarker identification for agarwood resin-loaded Aquilaria malaccensis

Cited by 5 publications

References 45 publications

Genome-wide analysis of respiratory burst oxidase homolog (Rboh) genes in Aquilaria species and insight into ROS-mediated metabolites biosynthesis and resin deposition

Genome-wide analysis of respiratory burst oxidase homolog (Rboh) genes in Aquilaria species and insight into ROS-mediated metabolites biosynthesis and resin deposition

Anatomical, chemical and endophytic fungal diversity of a Qi-Nan clone of Aquilaria sinensis (Lour.) Spreng with different induction times

Genome-wide investigation of Cytochrome P450 superfamily of Aquilaria agallocha: Association with terpenoids and phenylpropanoids biosynthesis

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