Transcript levels of <i>AtMRPs</i> after cadmium treatment: induction of <i>AtMRP3</i>

Bovet, Lucien; Eggmann, Thomas; Meylan-Bettex, Marlyse; Polier, J.; Kammer, Peter M.; Marín, Elena; Feller, Urs; Martinoia, Enrico

doi:10.1046/j.1365-3040.2003.00968.x

Cited by 111 publications

(77 citation statements)

References 57 publications

(101 reference statements)

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“…In the yeast, ABC transporters are involved in the vacuolar sequestration of Cd (Ortiz et al 1992;Wemmie et al 1994). Bovet et al (2003) studied the expression of several AtMRP genes in Cd-exposed A. thaliana. The expression of four genes, including AtMRP4 and AtMRP10, was up-regulated exclusively in the roots where Cd is retained, but expression was also found in the shoots.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Zn-responsive genes from two accessions of the hyperaccumulator plant Thlaspi caerulescens

Hassinen

Tervahauta

Halimaa

et al. 2006

Planta

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Several populations with diVerent metal tolerance, uptake and root-to-shoot transport are known for the metal hyperaccumulator plant Thlaspi caerulescens. In this study, genes diVerentially expressed under various Zn exposures were identiWed from the shoots of two T. caerulescens accessions (calaminous and non-calaminous) using Xuorescent diVerential display RT-PCR. cDNA fragments from 16 Zn-responsive genes, including those encoding metallothionein (MT) type 2 and type 3, MRP-like transporter, pectin methylesterase (PME) and Ole e 1-like gene as well as several unknown genes, were eventually isolated. The fulllength MT2 and MT3 sequences diVer from those previously isolated from other Thlaspi accessions, possibly representing new alleles or isoforms. Besides the diVerential expression in Zn exposures, the gene expression was dependent on the accession. Thlaspi homologues of ClpP protease and MRP transporter were induced at high Zn concentrations. MT2 and PME were expressed at higher levels in the calaminous accession. The MTs and MRP transporter expressed in transgenic yeasts were capable of conferring Cu and Cd tolerance, whereas the Ole e 1-like gene enhanced toxicity to these metals. The MTs increased yeast intracellular Cd content. As no signiWcant diVerences were found between Arabidopsis and Thlaspi MTs, they apparently do not diVer in their capacity to bind metals. However, the higher levels of MT2 in the calaminous accession may contribute to the Zn-adapted phenotype.

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Section: Discussionmentioning

confidence: 99%

Isolation of Zn-responsive genes from two accessions of the hyperaccumulator plant Thlaspi caerulescens

Hassinen

Tervahauta

Halimaa

et al. 2006

Planta

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“…Only one protein, an MRP-like ABC transporter, exhibited an increased protein ratio in the low and high Cd 2+ experiments. Interestingly, this MRP exhibits the highest homology to AtMRP3, the Arabidopsis MRP, which responds most strongly to Cd 2+ treatment in Arabidopsis [17]. AtMRP3 partially restores Cd 2+ tolerance in a Cd 2+ -sensitive yeast, suggesting that it is involved in vacuolar Cd 2+ transport.…”

Section: Atmrp3 Is Upregulated Under Low and High CD 2+mentioning

confidence: 97%

“…Cd 2+ was extracted by a 1.8 M H 2 SO 4 treatment and analyzed as described [17]. To determine Cd 2+ content in the growth substrates, 50 g of Vermiculite was centrifuged at 3000 g for 10 min.…”

Section: Determination Of Free CD 2+mentioning

confidence: 99%

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Quantitative detection of changes in the leaf‐mesophyll tonoplast proteome in dependency of a cadmium exposure of barley (Hordeum vulgare L.) plants

et al. 2009

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Although the vacuole is the most important final store for toxic heavy metals like cadmium (Cd(2+)), our knowledge on how they are transported into the vacuole is still insufficient. It has been suggested that Cd(2+) can be transported as phytochelatin-Cd(2+) by an unknown ABC transporter or in exchange with protons by cation/proton exchanger (CAX) transporters. To unravel the contribution of vacuolar transporters to Cd(2+) detoxification, a quantitative proteomics approach was performed. Highly purified vacuoles were isolated from barley plants grown under minus, low (20 microM), and high (200 microM) Cd(2+ )conditions and protein levels of the obtained tonoplast samples were analyzed using isobaric tag for relative and absolute quantitation (iTRAQ). Although 56 vacuolar transporter proteins were identified, only a few were differentially expressed. Under low-Cd(2+) conditions, an inorganic pyrophosphatase and a gamma-tonoplast intrinsic protein (gamma-TIP) were up-regulated, indicating changes in energization and water fluxes. In addition, the protein ratio of a CAX1a and a natural resistance-associated macrophage protein (NRAMP), responsible for vacuolar Fe(2+) export was increased. CAX1a might play a role in vacuolar Cd(2+) transport. An increase in NRAMP activity leads to a higher cytosolic Fe(2+) concentration, which may prevent the exchange of Fe(2+) by toxic Cd(2+). Additionally, an ABC transporter homolog to AtMRP3 showed up-regulation. Under high Cd(2+) conditions, the plant response was more specific. Only a protein homologous to AtMRP3 that showed already a response under low Cd(2+) conditions, was up-regulated. Interestingly, AtMRP3 is able to partially rescue a Cd(2+)-sensitive yeast mutant. The identified transporters are good candidates for further investigation of their roles in Cd(2+) detoxification. AbstractAlthough the vacuole is the most important final store for toxic heavy metals like cadmium (Cd 2+ ), our knowledge on how they are transported into the vacuole is still scarce. It has been suggested that Cd 2+ can be transported either as phytochelatin-Cd 2+ by an unknown ABC transporter or in exchange with protons by CAX transporters. To unravel the contribution of vacuolar transporters to Cd 2+ detoxification, a quantitative proteomics approach was performed.Highly purified vacuoles were isolated from barley plants grown under minus, low (20 µM), and high (200 µM) Cd 2+ conditions and protein levels of the obtained tonoplast samples were analyzed using iTRAQ TM . Although, 56 vacuolar transporter proteins were identified, however only a few were differentially expressed. Under low-Cd 2+ conditions an inorganic pyrophosphatase and a γ-tonoplast intrinsic protein (γ-TIP) were upregulated, indicating changes in energization and water fluxes. In addition, the protein ratio of a calcium/proton exchanger (CAX1a) and an NRAMP, responsible for vacuolar Fe 2+ export were increased. CAX1a might play a role in vacuolar Cd 2+ transport. An increase in NRAMP activity leads to a higher cytosol...

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“…Several studies have indicated that ABC transporters could be involved in cadmium efflux, transport, sequestration and ⁄ or redistribution (Bovet et al, 2003(Bovet et al, , 2005Kim et al, 2007). The P-type ion pump HMA4 has been studied extensively (Verret et al, 2004(Verret et al, , 2005Mills et al, 2005;Talke et al, 2006;Courbot et al, 2007;Hanikenne et al, 2008).…”

Section: Researchmentioning

confidence: 99%

The Brassica juncea BjCdR15, an ortholog of Arabidopsis TGA3, is a regulator of cadmium uptake, transport and accumulation in shoots and confers cadmium tolerance in transgenic plants

et al. 2009

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Summary• A bZIP transcription factor from Brassica juncea (BjCdR15) was isolated by the cDNA-amplified fragment length polymorphism technique after cadmium treatment. Sequence analysis indicated high similarity between BjCdR15 and Arabidopsis TGA3. In Arabidopsis, TGA3 transcription is also induced by cadmium; hence, we investigated whether BjCdR15 is involved in cadmium tolerance and whether it can functionally replace TGA3 protein in Arabidopsis tga3-2 mutant plants.• BjCdR15 expression was detected mainly in the epidermis and vascular system of cadmium-treated plants, and increased in roots and leaves after cadmium treatment. The overexpression of BjCdR15 in Arabidopsis and tobacco enhanced cadmium tolerance: overexpressing plants showed high cadmium accumulation in shoots. Conversely, Arabidopsis tga3-2 mutant plants showed high cadmium content in roots and inhibition of its transport to the shoot.• We demonstrated that BjCdR15 can functionally replace TGA3: in 35S:: BjCdR15-tga3-2 plants, the long-distance transport of cadmium from root to shoot was restored and these plants showed an increased cadmium content in shoots compared with all other assays. In addition, BjCdR15 ⁄ TGA3 regulated the synthesis of phytochelatin synthase and the expression of several metal transporters.• The results indicate that BjCdR15 ⁄ TGA3 transcription factors play a crucial role in the regulation of cadmium uptake by roots and in its long-distance root to shoot transport. BjCdR15 ⁄ TGA3 may thus be considered as useful candidates for potential biotechnological applications in the phytoextraction of cadmium from polluted soils.

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Transcript levels of AtMRPs after cadmium treatment: induction of AtMRP3

Cited by 111 publications

References 57 publications

Isolation of Zn-responsive genes from two accessions of the hyperaccumulator plant Thlaspi caerulescens

Isolation of Zn-responsive genes from two accessions of the hyperaccumulator plant Thlaspi caerulescens

Quantitative detection of changes in the leaf‐mesophyll tonoplast proteome in dependency of a cadmium exposure of barley (Hordeum vulgare L.) plants

The Brassica juncea BjCdR15, an ortholog of Arabidopsis TGA3, is a regulator of cadmium uptake, transport and accumulation in shoots and confers cadmium tolerance in transgenic plants

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