Transcript level characterization of a cDNA encoding stress regulated NAC transcription factor in the mangrove plant Avicennia marina

Govindan, Ganesan; Sankararamasubramanian, H. M.; Narayanan, Jithesh Mundaya; Sivaprakash, K. R.; Parida, Ajay

doi:10.1016/j.plaphy.2008.05.002

Cited by 22 publications

(11 citation statements)

References 31 publications

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“…Both OsNAC5 and ONAC010 belong to group A and share a complete NAC DNA-binding domain (Wve motifs named a-e, as seen in Fig. 4) and a variable transcriptional regulation domain Ganesan et al 2008). We were able to detect low expression of ONAC010 in panicles and conWrm the eYciency and speciWcity of the primers used.…”

Section: Nac Domain Analysismentioning

confidence: 57%

“…4). According to Fang et al (2008), the rice NAC family can be classiWed into Wve groups (I-V), and phylogenetic analysis revealed that both OsNAC5 and ONAC010 belong to the group III (namely stress-responsive NAC genes, Ganesan et al 2008). Both OsNAC5 and ONAC010 belong to group A and share a complete NAC DNA-binding domain (Wve motifs named a-e, as seen in Fig.…”

Section: Nac Domain Analysismentioning

confidence: 99%

“…4 Amino acid alignment of the NAC domain of ONAC010 (Os07g37920), TtNAM-B1 (DQ869673) and OsNAC5 (Os11g08210). The Wve NAC conserved motifs are indicated by black bars a, b, c, d and e Ganesan et al 2008). The secondary structure of OsNAC5 is shown under the alignment.…”

Section: Osnac5 Expression In Xag Leaves and Paniclesmentioning

confidence: 99%

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Identification of up-regulated genes in flag leaves during rice grain filling and characterization of OsNAC5, a new ABA-dependent transcription factor

Sperotto

Ricachenevsky

Duarte

et al. 2009

Planta

192

153

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Rice is a poor source of micronutrients such as iron and zinc. To help clarify the molecular mechanisms that regulate metal mobilization from leaves to developing seeds, we conducted suppression subtractive hybridization analysis in flag leaves of two rice cultivars. Flag leaves are the major source of remobilized metals for developing seeds. We isolated 78 sequences up-regulated in flag leaves at the grain filling stage relative to the panicle exertion stage. Differential expression of selected genes (encoding 7 transport proteins, the OsNAS3 enzyme and the OsNAC5 transcription factor) was confirmed by quantitative RT-PCR. We show that OsNAC5 expression is up-regulated by natural (aging) and induced senescence processes (dark, ABA application, high salinity, cold and Fe-deficiency) and its expression is not affected in the presence of 6-benzylaminopurine (a senescence inhibitor) under dark-induced senescence. Salt induction of OsNAC5 expression is abolished by nicotinamide, an inhibitor of ABA effects. This result and the presence of cis-acting elements in the promoter region of the OsNAC5 gene suggest an ABA-dependent regulation. Using four different rice cultivars, we show that OsNAC5 up-regulation is higher and earlier in flag leaves and panicles of IR75862 plants, which have higher seed concentrations of Fe, Zn and protein. We suggest that OsNAC5 is a novel senescence-associated ABA-dependent NAC transcription factor and its function could be related to Fe, Zn and amino acids remobilization from green tissues to seeds.

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Section: Nac Domain Analysismentioning

confidence: 57%

Section: Nac Domain Analysismentioning

confidence: 99%

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Identification of up-regulated genes in flag leaves during rice grain filling and characterization of OsNAC5, a new ABA-dependent transcription factor

Sperotto

Ricachenevsky

Duarte

et al. 2009

Planta

192

153

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“…NAC transcription factors are diverse in function and responsive to biotic and abiotic stresses. AmNAC1, a NAC transcription factor from Avicennia marina, up-regulated upon salt and ABA hormone treatments (Ganesan et al 2008). Recently a number of NAC transcription factors (i.e., NAC1, NAC2, osNAC4) was reported to be differentially expressed in response to both slow and shock drought stress in wild emmer wheats ).…”

Section: Mirnas Play Important Role In Foliar Glyphosate Applicationmentioning

confidence: 99%

Genome-wide profiling and analysis of Festuca arundinacea miRNAs and transcriptomes in response to foliar glyphosate application

et al. 2010

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Glyphosate is a broad spectrum herbicide which has been widely used for non-selective weed control in turfgrass management. Festuca arundinacea cv. Falcon was shown to be one of the tolerant turfgrass species in response to varying levels of glyphosate [5% (1.58 mM), 20% (6.32 mM)] recommended for weed control. However, there is a lack of knowledge on the mRNA expression patterns and miRNA, critical regulators of gene expression, in response to varying levels of glyphosate treatments. Here, we investigate the transcriptome and miRNA-guided post-transcriptional networks using plant miRNA microarray and Affymetrix GeneChip Wheat Genome Array platforms. Transcriptome analysis revealed 93 up-regulated and 78 down-regulated genes, whereas a smaller number showed inverse differential expressions. miRNA chip analysis indicated a number of (34 out of the 853) plant miRNAs were differentially regulated in response to glyphosate treatments. Target transcripts of differentially regulated miRNAs were predicted and nine of them were quantified by quantitative real-time PCR (qRT-PCR). Target transcripts of miRNAs validate the expression level change of miRNAs detected by miRNA microarray analysis. Down-regulation of miRNAs upon 5 and 20% glyphosate applications led to the up-regulation of their target observed by qRT-PCR or vice versa. Quantification of F. arundinacea miRNA, homologous of osa-miR1436, revealed the agreement between the Affymetrix and miRNA microarray analyses. In addition to miRNA microarray experiment, 25 conserved F. arundinacea miRNAs were identified through homology-based approach and their secondary structures were predicted. The results presented serve as analyses of genome-wide expression profiling of miRNAs and target mRNAs in response to foliar glyphosate treatment in grass species.

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“…NAC proteins form a large family of plant-specific DNA binding transcription factors that are gaining importance in recent times with respect to understanding plant development and adaptation. In A. marina, AmNAC1 transcript expression was upregulated by NaCl and ABA treatment [97].…”

Section: Genes For Signal Transductionmentioning

confidence: 97%

Molecular Breeding for Enhancing Abiotic Stress Tolerance Using Halophytes

Parida

George

Kavita

2012

Improving Crop Resistance to Abiotic Stress

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Transcript level characterization of a cDNA encoding stress regulated NAC transcription factor in the mangrove plant Avicennia marina

Cited by 22 publications

References 31 publications

Identification of up-regulated genes in flag leaves during rice grain filling and characterization of OsNAC5, a new ABA-dependent transcription factor

Identification of up-regulated genes in flag leaves during rice grain filling and characterization of OsNAC5, a new ABA-dependent transcription factor

Genome-wide profiling and analysis of Festuca arundinacea miRNAs and transcriptomes in response to foliar glyphosate application

Molecular Breeding for Enhancing Abiotic Stress Tolerance Using Halophytes

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