Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy

The Composition of the Arabidopsis RNA Polymerase II Transcript Elongation Complex Reveals the Interplay between Elongation and mRNA Processing Factors

“…In the first two columns, the average Mascot scores are given as well as the number of times the interactor was detected in three independent affinity purifications. copurified comprised TFIIS, SPT4/SPT5, PAF1-C, and FACT, and to a lesser extent Elongator, which are all implicated in the regulation of Arabidopsis transcript elongation (Dürr et al, 2014;Grasser et al, 2009;He et al, 2004;Lolas et al, 2010;Nelissen et al, 2010;Oh et al, 2008). SPT6L and SPT6 were also identified in several TEF eluates, which is consistent with the colocalization of SPT6L with elongating RNAPII and its preferential binding to Ser2-phosphorylated CTD repeats.…”

“…The double mutants, along with the respective single mutants and Col-0 wild-type plants, were primarily examined regarding growth and developmental phenotypes for which differences were observed in the parental lines. We generated plants deficient in TFIIS (which have an essentially wild-type appearance; Grasser et al, 2009) and the FACT subunits (ssrp1 and spt16, which express reduced amounts of the FACT subunits and similarly display various developmental defects; Lolas et al, 2010). While the tfIIs ssrp1 double mutants are synergistically affected regarding rosette diameter and number of primary inflorescences (Figures 4A to 4C; Supplemental Figure 4A), the tfIIs spt16 plants are comparable to the spt16 single mutants (Figures 4D to 4F; Supplemental Figure 4B).…”

“…The Elongator complex has histone acetyl-transferase activity and modulates developmental and immune response pathways (Woloszynska et al, 2016). TFIIS assists RNAPII progression of transcription through various obstacles and is involved in the expression of DOG1, a key regulator of Arabidopsis seed dormancy (Grasser et al, 2009;Mortensen and Grasser, 2014). Another factor altering the performance of RNAPII is the SPT4/SPT5 heterodimer, which is thought to render the elongation complex stable and processive (Martinez-Rucobo and Cramer, 2013).…”

Evolution, biogenesis and function of promoter-associated RNAs

“…8 Intriguingly, TFIIS is not essential for yeast viability 44,45 and plants defective in TFIIS show only a mild reduced seed dormancy phenotype, 46 whereas homozygous null mutant TFIIS mice die at midgestation. 47 This may reflect an important role for TFIIS in gene regulation through tiRNA biogenesis in developmentally complex animals.…”

UV Damaged DNA Repair & Tolerance in Plants

“…Elucidation of the TC-NER mechanism in plants is still at its infancy. While there is no plant homologue for HMGN1, the Arabidopsis genome encodes homologues of XAB2, TFIIS, and five p300/CBP homologues, however the role of these genes in DNA repair has not been assessed (Grasser et al, 2009;Kunz et al, 2005;Pandey et al, 2002). Only recently was the homolog of human CSA cloned and characterized in Arabidopsis.…”