Transcript analysis of 203 novel genes fromSaccharomyces cerevisiaeinhap1androx1mutant backgrounds

Abstract

Hap1 and Rox1 are transcriptional regulators that bind regulatory sites in the promoters of oxygen-regulated genes in Saccharomyces cerevisiae. Hap1 is a heme-responsive activator of genes induced in aerobic conditions and Rox1 is a repressor of hypoxic genes in aerobic conditions. We have studied transcriptional regulation of a pool of 203 open reading frames (ORFs) from chromosomes IV, VII, and XIV in wild-type, hap1, and rox1 mutant genetic backgrounds in an attempt to extend the family of oxygen and heme regulated genes. Only three ORFs are significantly repressed by Rox1 but they cannot be considered as typical hypoxic genes because they are not overexpressed during hypoxia.

Impact of DNA-binding position variants on yeast gene expression

“…Another example is HAP1, which acts as repressor in the gene encoding function related to respiration and oxidative damage (43). In our work, position pair (2, 8) with (C, T) in the HAP1 motif is considered functional in the diauxic condition, which is consistent with the function attributed to HAP1 in previous experiments (44,45). Moreover, we found that several position pairs in HAP1 were functional under experimental conditions related to the cell cycle, which is consistent with previous findings (46).…”

Time-Course Analysis of Gene Expression During theSaccharomyces cerevisiaeHypoxic Response

“…For example, several studies have found that CYC1 , encoding cytochrome c, is downregulated, while HEM13 , encoding a heme biosynthetic enzyme, is upregulated (Zhang and Hach 1999; Lombardía et al 2000; Ter Linde and Steensma 2002; Hickman and Winston 2007; Butler 2013). However, other genes exhibit conflicting responses between studies.…”

Transcriptional response to mitochondrial NADH kinase deficiency in Saccharomyces cerevisiae

“…Also evident in Fig. 2 are the numerous genes associated with anaerobic/hypoxic growth (Lombardía et al, 2000; Abramova et al, 2001; Becerra et al, 2002; Kwast et al, 2002; Ter Linde and Steensma, 2002; Lai et al, 2005; Lai et al, 2006). ANB1 , encoding an anaerobically-induced transcription factor (Sertil et al, 2003), was up-regulated 7.9-fold in pos5 cells while cell wall mannoprotein gene DAN1 was up-regulated 12.7-fold and HES1 , associated with the regulation of ergosterol biosynthesis, was up-regulated 5.3-fold (Table 1).…”

“…Shianna et al (Shianna et al, 2006) similarly reported the up-regulation of genes associated with anaerobiosis or hypoxia in their strain S288c-based pos5 ∷kanMX hap1 ∷Ty1 double mutant cells. While transcriptional changes in gene expression associated with hypoxic or anaerobic cell growth are well-known (Lombardía et al, 2000; Abramova et al, 2001; Becerra et al, 2002; Kwast et al, 2002; Ter Linde and Steensma, 2002; Lai et al, 2005; Lai et al, 2006), we extended the list of hypoxia-related genes differentially expressed in pos5 cells to include AAC3, ANB1, AUS1, DAN1 and HEM13 , each of which were up-regulated in pos5 cells (Table 1). The changes in expression affecting iron sensing and transport genes described by Shianna et al (Shianna et al, 2006) were not observed in the present study and are therefore more likely related to the hap1 ∷Ty1 mutation and its effects on mitochondrial heme biosynthesis, than due to the pos5 ∷kanMX mutation.…”

“…HAP1 encodes the heme activator protein, a transcription factor involved in the complex regulation of gene expression in response to levels of heme and oxygen (Zitomer and Lowry, 1992; Zhang and Hach, 1999; Lombardía et al, 2000; Becerra et al, 2002; Ter Linde and Steensma, 2002; Emerling and Chandel, 2005; Hon et al, 2005). Many S. cerevisiae laboratory strains, including S288c and derivatives including BY4741 (Mortimer and Johnston, 1986; Brachmann et al, 1998), contain a Ty1 transposon insertion in HAP1 (Gaisne et al, 1999).…”