Transcranial low-level laser therapy (810 nm) temporarily inhibits peripheral nociception: photoneuromodulation of glutamate receptors, prostatic acid phophatase, and adenosine triphosphate

Sousa, Marcelo Victor Pires de; Ferraresi, Cleber; Kawakubo, Masayoshi; Kaippert, Beatriz; Yoshimura, Elisabeth Mateus; Hamblin, Michael R.

doi:10.1117/1.nph.3.1.015003

Cited by 32 publications

(31 citation statements)

References 42 publications

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“…We previously showed [7] that applying PBM with an 810 nm laser spot delivered to the head of the mouse, markedly raised the pain threshold in the hind foot with a maximum effect measured 2 hours later. In that study we also showed an analgesic effect for the response to cold, heat and inflammatory stimuli.…”

Section: Discussionmentioning

confidence: 99%

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Pain management using photobiomodulation: Mechanisms, location, and repeatability quantified by pain threshold and neural biomarkers in mice

Sousa

Kawakubo

Ferraresi

et al. 2018

Journal of Biophotonics

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Photobiomodulation (PBM) is a simple, efficient and cost-effective treatment for both acute and chronic pain. We previously showed that PBM applied to the mouse head inhibited nociception in the foot. Nevertheless, the optimum parameters, location for irradiation, duration of the effect and the mechanisms of action remain unclear. In the present study, the pain threshold in the right hind paw of mice was studied, after PBM (810 nm CW laser, spot size 1 or 6 cm , 1.2-36 J/cm ) applied to various anatomical locations. The pain threshold, measured with von Frey filaments, was increased more than 3-fold by PBM to the lower back (dorsal root ganglion, DRG), as well as to other neural structures along the pathway such as the head, neck and ipsilateral (right) paw. On the other hand, application of PBM to the contralateral (left) paw, abdomen and tail had no effect. The optimal effect occurred 2 to 3 hours post-PBM and disappeared by 24 hours. Seven daily irradiations showed no development of tolerance. Type 1 metabotropic glutamate receptors decreased, and prostatic acid phosphatase and tubulin-positive varicosities were increased as shown by immunofluorescence of DRG samples. These findings elucidate the mechanisms of PBM for pain and provide insights for clinical practice.

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Section: Discussionmentioning

confidence: 99%

“…Photobiomodulation therapy (PBMT) also known as low-level laser/light therapy (LLLT), is a rapidly growing approach to stimulate healing [5, 6], increase tissue regeneration [6] and reduce pain [7] and inflammation [8]. PBMT has no known side-effects, and as yet has shown no long-term adaptation to the treatment [9, 10].…”

Section: Introductionmentioning

confidence: 99%

Pain management using photobiomodulation: Mechanisms, location, and repeatability quantified by pain threshold and neural biomarkers in mice

Sousa

Kawakubo

Ferraresi

et al. 2018

Journal of Biophotonics

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“…1,3,[9][10][11][12][13] Many studies investigated the effectiveness of the transcranial NIR-PBM in clinical and normal conditions. [14][15][16][17][18] Improvement in the neurological or psychological symptoms has been reported after light irradiation in both animal and human models. 3,[19][20][21][22][23][24] The promotion of cognitive performance such as the reaction time has been observed in some cognitive studies assessed by the attentional tests or the psychomotor vigilance task (PVT).…”

Section: Introductionmentioning

confidence: 99%

Short-term Effects of Transcranial Near-Infrared Photobiomodulation on Motor Performance in Healthy Human Subjects: An Experimental SingleBlind Randomized Clinical Trial

et al. 2019

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Introduction: Transcranial near-infrared photobiomodulation (NIR-PBM) is a new noninvasive procedure which transcranially applies a near-infrared wavelength to the scalp with a laser or a light-emitting diode (LED) source. Improvement in the neurological or psychological symptoms has been reported following light irradiation. However, to our knowledge, there is no study to investigate the effects of transcranial NIR-PBM on motor performance directly. Therefore, the objective of this study was to investigate the short-term effects of transcranial NIR-PBM on motor performance in healthy human subjects. Methods: In this experimental single-blind randomized clinical trial study, 56 right-handed healthy participants, whose ages ranged from 18 to 30, were randomly assigned to (1) Real transcranial NIR-PBMC3 group (n=14), (2) Sham transcranial NIR-PBMC3 group (n=14), (3) Real transcranial NIR-PBMC4 group (n=14), and (4) Sham transcranial NIR-PBMC4 group (n=14). We applied the 808 nm laser with irradiation energy density of 60 J/cm2 and power density of 200 mw/cm2 to the C3 or C4 points of the scalp. The number of finger taps as an indicator of motor performance was assessed by the finger-tapping test (FTT) before and after irradiation of transcranial NIR-PBM on the corresponding points of the scalp for 5 minutes. Results: The results showed that the number of finger taps in both right and left hands following the use of transcranial NIR-PBM in the real transcranial NIR-PBMC3 group significantly increased (P<0.05). Conclusion: We concluded that using transcranial NIR-PBM with a laser source on C3 point of the motor cortex in right-handed healthy people can increase the number of finger taps in both hands as an indicator of motor performance improvement.

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“…Odontology [4]- [7] and Physiotherapy [8]. The best-known effects of the PBMT, which applies red and infrared lasers or LEDs in the treatments, are the reduction of inflammatory processes [9], improvement of wound healing [10], and reduction of pain [11].…”

Section: Introductionmentioning

confidence: 99%

“…To scan the whole specimen, either the specimen holder or the light source is moved. In the second design, on the bottom of the figure, calledepi-illuminated confocal microscope, there is only one lens(11), which works as condenser and objective lens. There is also a partial or dichromatic mirror(17).…”

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confidence: 99%

Investigation of the influence of red and infrared illumination on mechanical properties of cells: Photobiomodulation

Magalhães¹

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The photobiomodulation therapy (PBMT) has many demonstrated applications in the health area including anti-inflammatory and wound healing effects. The main objective of this work is to verify if the PBMT causes measurable changes in the mechanical properties of cells, specifically in red blood cells, epithelial cells and fibroblasts. In addition, to contribute to the knowledge of the action mechanisms of the PBMT, this study intends to support applications of the PBMT during invasive procedures, such as the direct photo-treatment of the blood in surgical procedures with cardiopulmonary bypass, regarding security of the cellular integrity. For this analysis, three experimental techniques were used: optical magnetic twisting cytometry (OMTC), defocusing microscopy and confocal laser-scanning microscopy. Human bronchial epithelial cells were evaluated with OMTC. The epithelial cell culture was either photo-treated or not, with red laser (λ=660 nm), and fixed power and time (power density of 153 mW/cm 2 , time 300 s). It was not possible to observe significant differences between photo-treated and control epithelial cells, for the hysteresivity (ratio between the cell loss and elastic shear moduli). The defocusing microscopy, similar to a phase contrast microscopy, was used to study human red blood cells from fresh blood. The red blood cells were either photo-treated or not, with red laser (λ=660 nm), and different powers and times (power densities from 0 to 510 mW/cm 2 , times from 0 to 180 s). Some morphological and mechanical characteristics of individual red blood cells were evaluated, such as volume, radial profile of cell thickness, lateral and vertical membrane fluctuations, for the photo-treated and control red blood cells. It was not possible to detect differences between the two groups, for any of the parameters analyzed. For both techniques, the absence of detectable differences might be due to several factors, such as the nonaction of the PBMT, with the parameters used, in the epithelial cells and red blood cells or to the small sensitivity of each technique. Confocal laser-scanning microscopy was used to evaluate the actin filaments of mouse fibroblasts. The fibroblast cell culture was either photo-treated or not, with red (λ=625 nm) or infrared (λ=808 nm) light and fixed power and time (power density from 113 to 158 mW/cm 2 , time 300 s). The nucleus and cell areas increased slightly when comparing photo-treated and control cells. On the other hand, the total actin, total actin density and the number of filaments decreased. These changes were detected for a short time after treatment, however, 10 after 24 h they are not anymore detectable. The total branch length does not seem to suffer any modifications. In summary, with the data acquired with the three techniques, it was found that the PBMT, in the red range, with the parameters used, could not cause noticeable changes in red blood cells and epithelial cells, in vitro. On the other hand, the PBMT in the red and near-infrared range, with the power and ...

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Transcranial low-level laser therapy (810 nm) temporarily inhibits peripheral nociception: photoneuromodulation of glutamate receptors, prostatic acid phophatase, and adenosine triphosphate

Cited by 32 publications

References 42 publications

Pain management using photobiomodulation: Mechanisms, location, and repeatability quantified by pain threshold and neural biomarkers in mice

Pain management using photobiomodulation: Mechanisms, location, and repeatability quantified by pain threshold and neural biomarkers in mice

Short-term Effects of Transcranial Near-Infrared Photobiomodulation on Motor Performance in Healthy Human Subjects: An Experimental SingleBlind Randomized Clinical Trial

Investigation of the influence of red and infrared illumination on mechanical properties of cells: Photobiomodulation

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