Background and Purpose-Right to left shunt is involved in conditions in which postural changes may be pathogenically relevant. The aim of this work was to assess the frequency of posturally dependent right to left shunt. Methods-In 109 consecutive right to left shunt-positive subjects (male/femaleϭ40/69, age 43Ϯ12 years), we assessed with contrast-enhanced transcranial Doppler the bubble load during normal breathing and after the Valsalva maneuver in both standing and recumbent position randomizing the order of testing. Results-During normal breathing, the average bubble count was 11Ϯ20 in the recumbent and 26Ϯ60 in the standing position. After the Valsalva maneuver, it was 40Ϯ38 and 42Ϯ37, respectively. The increase of bubble load in standing position occurred in 42% of patients and was independent of the order of testing. Conclusions-The amount of permanent right to left shunt is posture-dependent in 40% of patients. Testing in the sitting position may thus be warranted in doubtful or inconclusive results obtained with the subject in the horizontal position.