Transbronchial real-time lung tumor localization with folate receptor–targeted near-infrared molecular imaging: A proof of concept study in animal models

Ishiwata, Tsukasa; Hiraishi, Yoshihisa; Bernards, Nicholas; Sata, Yuki; Gregor, Alexander; Aragaki, Masato; Yasufuku, Kazuhiro

doi:10.1016/j.jtcvs.2022.09.042

Cited by 2 publications

(1 citation statement)

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“…Therefore, it could be used as an important cancer biomarker and its level could be used to predict cancer stages [14,15]. FRs have been detected in tissue samples using different techniques, such as surface enhanced Raman spectroscopy (SERS) [16], proteomics assays [17], fluorescence imaging [18,19], and positron emission tomography (PET) [20,21]. However, these techniques are tedious, costly, time consuming, pose a risk to human health due to toxic radioligands, and have low sensitivity, such as FA-fluorescein isothiocyanate dye, which has a short systemic circulation time, and therefore does not target tumors efficiently [22].…”

Section: Introductionmentioning

confidence: 99%

Impedimetric Detection of Cancer Markers Based on Nanofiber Copolymers

Elnagar,

Elgiddawy,

El Rouby

et al. 2024

Biosensors

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The sensitive determination of folate receptors (FRs) in the early stages of cancer is of great significance for controlling the progression of cancerous cells. Many folic acid (FA)-based electrochemical biosensors have been utilized to detect FRs with promising performances, but most were complicated, non-reproducible, non-biocompatible, and time and cost consuming. Here, we developed an environmentally friendly and sensitive biosensor for FR detection. We proposed an electrochemical impedimetric biosensor formed by nanofibers (NFs) of bio-copolymers prepared by electrospinning. The biosensor combines the advantages of bio-friendly polymers, such as sodium alginate (SA) and polyethylene oxide (PEO) as an antifouling polymer, with FA as a biorecognition element. The NF nanocomposites were characterized using various techniques, including SEM, FTIR, zeta potential (ZP), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). We evaluated the performance of the NF biosensor using EIS and demonstrated FR detection in plasma with a limit of detection of 3 pM. Furthermore, the biosensor showed high selectivity, reliability, and good stability when stored for two months. This biosensor was constructed from ‘green credentials’ holding polymers that are highly needed in the new paradigm shift in the medical industry.

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