Transauricular intra-arterial and intravenous digital subtraction angiography for abdominal aortic aneurysm imaging in a rabbit model

Bi, Yonghua; Yu, Zepeng; Xu, Ke; Zhong, Hongshan; Wu, Gang; Han, Xinwei

doi:10.1016/j.amsu.2017.05.004

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Rabbits underwent intravenous digital subtraction angiography (IVDSA) imaging after 1, 3, 7, and 15 weeks similar to previous reports [ 9 , 12 , 13 , 20 ]. Specifically, a 22-G angiocatheter was placed in the ear vein for 5s DSA scanning (Siemens Artis Zee, Germany).…”

Section: Methodsmentioning

confidence: 77%

Rabbit aortic aneurysm model with enlarging diameter capable of better mimicking human aortic aneurysm disease

Chen

et al. 2018

PLoS ONE

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The self-healing phenomenon can be found in the elastase-induced abdominal aortic aneurysm (AAA) model, and an enlarging AAA model was successfully induced by coarctation. Unfortunately, aortic coarctation in these enlarging models is generally not found in human AAA disease. This study aimed to create an experiment model of enlarging AAA in rabbits to better mimic human aortic aneurysm disease. Eighty-four male New Zealand white rabbits were randomly divided into three equal groups: two aneurysm groups (A and B) and a SHAM group. Aneurysm group rabbits underwent extrinsic aortic stenosis below the right renal artery and received a 10-minute incubation of 60 μl elastase (1 unit/μl). Absorbable suture was used in Group A and nonabsorbable cotton thread was used in Group B. A sham operation was performed in the SHAM group. Aortic diameter was measured after 1, 3, 7, and 15 weeks; thereafter animals were sacrificed for histopathological, immunohistochemical and quantitative studies. Two rabbits died at 29 and 48 days, respectively, after operation in Group B. All aneurysms formed and enlarged progressively by 3 weeks in the Aneurysm groups. However, diameter enlargement in Group A was significantly lower than that in Group B at 7 weeks. Aneurysm groups developed intimal hyperplasia; intima-media thickness (IMT) increased significantly by week 7, and aortic media thickness and intima-media ratio (IMR) increased significantly by week 15. Marked destruction of elastin fibers and smooth muscle cells (SMCs) occurred 1 week later and increased progressively thereafter. Intimal hyperplasia and SMCs content in Group A increased significantly by week 15 compared with Group B. Aneurysm groups exhibited strong expression of matrix metalloproteinases 2 and 9 and RAM11 by week 1, and decreased progressively thereafter. In conclusion, this novel rabbit AAA model enlarges progressively without coarctation and is capable of better mimicking human aortic aneurysm disease.

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Section: Methodsmentioning

confidence: 77%

Rabbit aortic aneurysm model with enlarging diameter capable of better mimicking human aortic aneurysm disease

Chen

et al. 2018

PLoS ONE

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“…All rabbits underwent 5s Digital subtraction angiography (DSA) scanning (Siemens Artis Zee, Germany) at 1, 3, 5, and 15 weeks. [ 14 ] The diameter measurement and 3D-DSA analysis were performed with RadiAnti DICOM Viewer 3.4.2 (Medixant Company, Poland). Four weeks later, all rabbit in Group A underwent balloon dilation.…”

Section: Methodsmentioning

confidence: 99%

Hemodynamics and pathology of an enlarging abdominal aortic aneurysm model in rabbits

Chen

et al. 2018

PLoS ONE

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Hemodynamics may play an essential role in the initiation and progression of abdominal aortic aneurysm (AAA). We aimed to study the mechanism of self-healing process by the changes of hemodynamics and pathology in an enlarging AAA in rabbits. Seventy-two rabbits were randomly divided into three groups. Rabbits underwent extrinsic coarctation and received a 10-minute elastase incubation in Group A and Group B. Absorbable suture used in Group A was terminated by balloon dilation at week 4. Diameter was measured after 1, 3, 5, and 15 weeks, computational fluid dynamics analysis was performed at week 3 and week 15. Rabbits were sacrificed after 1, 5, and 15 weeks for pathological and quantitative studies. The higher velocity magnitude, intensified bulk flow and obvious vortex formation were observed in Group A at week 3 instead of week 15. Both low wall shear stress and high relative residence time increased in Group B, however, high oscillatory shear index had relatively less increase compared with Group A. Aortic diameter reached a plateau at 5 weeks in Group A, which was significantly lower than in week 15 in Group B. Intimal hyperplasia, intima-media thickness increased significantly in Group A at week 5, significantly higher than in week 15 in Group B. Marked destruction of elastin fibers and smooth muscle cells occurred at week 1, and increased significantly at week 15 in Group A. Aneurysm exhibited strong expression of matrix metalloproteinase 9 and mouse anti-rabbit macrophage 11 at week 1, and showed a tendency to decrease. Matrix metalloproteinase 2 expression decreased significantly in Group B at week 15 compared with week 5 and Group A. In conclusion, the self-healing of rabbit AAA may attributed to the regeneration of smooth muscle cells. The turbulence flow caused by coarctation is associated with continuous growth of rabbit AAA and prevents the self-healing phenomenon.

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Degeneration and Regeneration of Smooth Muscle Cells in Two Different Abdominal Aortic Aneurysm Models in Rabbits

Guo

et al. 2022

Annals of Vascular Surgery

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Transauricular intra-arterial and intravenous digital subtraction angiography for abdominal aortic aneurysm imaging in a rabbit model

Cited by 3 publications

References 19 publications

Rabbit aortic aneurysm model with enlarging diameter capable of better mimicking human aortic aneurysm disease

Rabbit aortic aneurysm model with enlarging diameter capable of better mimicking human aortic aneurysm disease

Hemodynamics and pathology of an enlarging abdominal aortic aneurysm model in rabbits

Degeneration and Regeneration of Smooth Muscle Cells in Two Different Abdominal Aortic Aneurysm Models in Rabbits

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