Transaminases for Green Chemistry: Recent Progress and Future Prospects

Pandya, Shreya; Gupte, Akshaya

doi:10.48022/mbl.2309.09009

Cited by 2 publications

(1 citation statement)

References 111 publications

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“…With a broad selectivity, enzymes or whole cells are very attractive catalyst tools for chemical synthesis in particular for valuable chiral compounds. [1,2] As an example, lipase-mediated production of the herbicide Dimethenamide-P by BASF allows the formation of a single enantiomer instead of the racemic mixture produced by a chemical process. [3] In addition to the improved selectivity of this reaction, the other major breakthrough with biocatalysis is in environmental sustainability compared to classical chemical synthesis, with mild operating conditions (e. g. aqueous media, low temperature and atmospheric pressure).…”

Section: Introductionmentioning

confidence: 99%

Wall‐Immobilized Biocatalyst vs. Packed Bed in Miniaturized Continuous Reactors: Performances and Scale‐Up

Michaud,

Nonglaton,

Anxionnaz‐Minvielle

2024

ChemBioChem

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Sustainable biocatalysis syntheses have gained considerable popularity over the years. However, further optimizations – notably to reduce costs – are required if the methods are to be successfully deployed in a range of areas. As part of this drive, various enzyme immobilization strategies have been studied, alongside process intensification from batch to continuous production. The flow bioreactor portfolio mainly ranges between packed bed reactors and wall‐immobilized enzyme miniaturized reactors. Because of their simplicity, packed bed reactors are the most frequently encountered at lab‐scale. However, at industrial scale, the growing pressure drop induced by the increase in equipment size hampers their implementation for some applications. Wall‐immobilized miniaturized reactors require less pumping power, but a new problem arises due to their reduced enzyme‐loading capacity. This review starts with a presentation of the current technology portfolio and a reminder of the metrics to be applied with flow bioreactors. Then, a benchmarking of the most recent relevant works is presented. The scale‐up perspectives of the various options are presented in detail, highlighting key features of industrial requirements. One of the main objectives of this review is to clarify the strategies on which future study should center to maximize the performance of wall‐immobilized enzyme reactors.

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Section: Introductionmentioning

confidence: 99%

Wall‐Immobilized Biocatalyst vs. Packed Bed in Miniaturized Continuous Reactors: Performances and Scale‐Up

Michaud,

Nonglaton,

Anxionnaz‐Minvielle

2024

ChemBioChem

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Two‐Step Biocatalytic Synthesis of (1S)‐Nor(pseudo)ephedrine Catalyzed by Immobilized Enzymes

Patti,

Magrini Alunno,

Semproli

et al. 2024

ChemCatChem

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Nor(pseudo)ephedrines [N(P)Es] are naturally occurring compounds showing sympathomimetic activity that have many applications especially in the chemical industry and in the pharmaceutical field. Recently a two‐step biocatalytic cascade for the preparation of (1S)‐N(P)E was designed, consisting of a benzoin‐type condensation catalyzed by the (S)‐selective acetoin:dichlorophenolindophenol oxidoreductase (Ao:DCPIP OR) followed by a transamination mediated by either a (S)‐ or (R)‐selective amine transaminase (ATA). In this study, we successfully immobilized both Ao:DCPIP OR and two ATAs of opposite enantioselectivity and used them in the biosynthetic cascade to N(P)Es. Immobilization yield, activity recovery, and stability of the immobilized enzymes, both after use (enzyme recycling) and storage (shelf‐life), were assessed. Ao:DCPIP OR immobilized on glyoxyl‐agarose exhibited remarkable stability under storage conditions throughout the 30‐days monitoring period. Moreover, it was successfully reused in the synthesis of (S)‐phenylacetylcarbinol [(S)‐PAC] yielding high conversion (>94%) and enantiomeric excess (>99%). The (R)‐selective At‐ATA from Aspergillus terreus, immobilized on Eupergit® C, demonstrated a slightly higher storage stability when compared to the (S)‐selective Sbv333‐ATA from Streptomyces sp. Bv333 immobilized on the same carrier. Remarkably, both enzymes were effectively reused for ten reaction cycles in the synthesis of N(P)Es, starting from the enzymatically synthesized (S)‐PAC, achieving complete conversions and excellent diastereoselectivity (>99%).

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Transaminases for Green Chemistry: Recent Progress and Future Prospects

Cited by 2 publications

References 111 publications

Wall‐Immobilized Biocatalyst vs. Packed Bed in Miniaturized Continuous Reactors: Performances and Scale‐Up

Wall‐Immobilized Biocatalyst vs. Packed Bed in Miniaturized Continuous Reactors: Performances and Scale‐Up

Two‐Step Biocatalytic Synthesis of (1S)‐Nor(pseudo)ephedrine Catalyzed by Immobilized Enzymes

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