“…As can be seen (Figure 4a), the inhibitory effect of pdcd4 expression on the extended u-PAR promoter was abolished with a promoter construct deleted for region À402/À350 which contains putative Sp1, NF-1 and GATA-2 sequences (Hapke et al, 2001). Next, we asked as to whether an AP-1 consensus motif (À190/À171, (Lengyel et al, 1996)), and a combined motif that bound an AP-2-like protein, Sp1 and Sp3 (Allgayer et al, 1999c) within the À398 bp basal u-PAR promoter, which we had characterized extensively in previous works, might participate in Pdcd4-mediated u-PAR promoter regulation. Transient transfections and CAT-assays of RKO cells with CAT-reporter-constructs driven by mutations abolishing the binding of AP-1 transcription factors to region À190/À171, Sp1/Sp3 to region À152/ À135, and AP-2/Sp1 and Sp3 to region À152/À135, respectively, were conducted in parallel to transfections with a wild-type-(À398)-u-PAR promoter-CAT reporter, and compared for their ability to abolish Pdcd4-induced promoter suppression ( Figure 4b).…”