Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2

Ding, Qiang; Jin, Min Kyung; Wang, Yaoyue; Liu, Jiao; Kalds, Peter; Wang, Ying; Yang, Yuxin; Wang, Xiaolong; Chen, Yulin

doi:10.3390/cells9020445

Cited by 23 publications

(19 citation statements)

References 49 publications

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“…Hence, these miRNAs may play a role in ovarian organogenesis and hormone secretion through oocyte meiosis. Besides, miR-202-5p and miR-485-3p were significantly upregulated in LF; they have been indicated to have a regulatory role in ovarian hormone metabolism and granulosa cell development [ 55 , 56 ]. In zebrafish, knocking out miR-202 impaired the early steps of oogenesis/folliculogenesis and decreased the number of large (i.e., vitellogenic) follicles, ultimately leading to no reproductive success [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

“…As the potential role of these miRNAs in ovarian maturation was predicted, further experiments were needed to verify their function. Specifically, as reported in the literature [ 56 ], a series of functional experiments must be taken to identify their location and their upstream/downstream regulators, in order to reveal the mechanisms of ovarian maturation.…”

Section: Discussionmentioning

confidence: 99%

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A Preliminary Study on the Characteristics of microRNAs in Ovarian Stroma and Follicles of Chuanzhong Black Goat during Estrus

Zou

Liu

et al. 2020

Genes

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microRNAs (miRNAs) play a significant role in ovarian follicular maturity, but miRNA expression patterns in ovarian stroma (OS), large follicles (LF), and small follicles (SF) have been rarely explored. We herein aimed to identify miRNAs, their target genes and signaling pathways, as well as their interaction networks in OS, LF, and SF of Chuanzhong black goats at the estrus phase using small RNA-sequencing. We found that the miRNA expression profiles of LF and SF were more similar than those of OS—32, 16, and 29 differentially expressed miRNAs were identified in OS vs. LF, OS vs. SF, and LF vs. SF, respectively. Analyses of functional enrichment and the miRNA-targeted gene interaction network suggested that miR-182 (SMC3), miR-122 (SGO1), and miR-206 (AURKA) were involved in ovarian organogenesis and hormone secretion by oocyte meiosis. Furthermore, miR-202-5p (EREG) and miR-485-3p (FLT3) were involved in follicular maturation through the MAPK signaling pathway, and miR-2404 (BMP7 and CDKN1C) played a key role in follicular development through the TGF-β signaling pathway and cell cycle; nevertheless, further research is warranted. To our knowledge, this is the first study to investigate miRNA expression patterns in OS, LF, and SF of Chuanzhong black goats during estrus. Our findings provide a theoretical basis to elucidate the role of miRNAs in follicular maturation. These key miRNAs might provide candidate biomarkers for the diagnosis of follicular maturation and will assist in developing new therapeutic targets for female goat infertility.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Preliminary Study on the Characteristics of microRNAs in Ovarian Stroma and Follicles of Chuanzhong Black Goat during Estrus

Zou

Liu

et al. 2020

Genes

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“…MicroRNA (miRNA) is a short (20-24 nt) non-coding RNA, which mainly binds to the 3'UTR of the target genes' mRNA sequence to stimulate degradation of mRNA, to regulate mRNA expression at the posttranscriptional level and inhibit its translation [13,14]. Many previous studies have demonstrated that miRNA regulates the biological function of GCs by its targets.…”

Section: Introductionmentioning

confidence: 99%

MiR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine granulosa cells

Shi

Zhou

et al. 2020

J Animal Sci Biotechnol

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Background Granulosa cells (GCs) proliferation and estradiol synthesis significantly affect follicular development. The miR-214-3p expression in the ovarian tissues of high-yielding sows is higher than that in low-yielding sows, indicating that miR-214-3p may be involved in sow fertility. However, the functions and mechanisms of miR-214-3p on GCs are unclear. This study focuses on miR-214-3p in terms of the effects on GCs proliferation and estradiol synthesis. Results Our findings revealed that miR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine GCs. MiR-214-3p can increase the percentage of S-phase cells, the number of EdU labeled positive cells, and cell viability. However, E2 concentration was reduced after miR-214-3p agomir treatment. We also found that miR-214-3p up-regulates the expression of cell cycle genes including cell cycle protein B (Cyclin B), cell cycle protein D (Cyclin D), cell cycle protein E (Cyclin E), and cyclin-dependent kinase 4 (CDK4) at the transcription and translation levels, but down-regulates the mRNA and protein levels of cytochrome P450 family 11 subfamily A member 1 (CYP11A1), cytochrome P450 family 19 subfamily A member 1 (CYP19A1), and steroidogenic acute regulatory protein (StAR) (i.e., the key enzymes in estradiol synthesis). On-line prediction, bioinformatics analysis, a luciferase reporter assay, RT-qPCR, and Western blot results showed that the target genes of miR-214-3p in proliferation and estradiol synthesis are Mfn2 and NR5A1, respectively. Conclusions Our findings suggest that miR-214-3p plays an important role in the functional regulation of porcine GCs and therefore may be a target gene for regulating follicular development.

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“…MicroRNA (miRNA) is a short (20-24nt) non-coding RNA, which mainly binds to the 3'UTR of the target gene's mRNA sequence to stimulate degradation of mRNA to regulate mRNA expression at the posttranscriptional level and inhibit its translation (13,14). Many previous studies have demonstrated that miRNA regulates the biological function of GCs by its targets.…”

Section: Introductionmentioning

confidence: 99%

“…For example, in mouse GCs, miRNA-746-3p targets steroidogenic factor-1 (SF-1) to regulate 17β-estradiol synthesis (15). MiR-202-5p induces apoptosis in goat GCs by targeting TGFβR2 (16). Another research proved that miR-1275 controls GCs apoptosis and estradiol synthesis by impairing LRH-1/CYP19A1 axis (17).…”

Section: Introductionmentioning

confidence: 99%

MiR-214-3p Promotes Proliferation and Inhibits Estradiol Synthesis In Porcine Granulosa Cells

Shi

Zhou

et al. 2020

Preprint

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Background: Granulosa cells proliferation and estradiol synthesis significantly affect follicular development. The miR-214-3p expression in the ovarian tissues of high-yielding sows is higher than that in low-yielding sows, indicating that miR-214-3p may be involved in sow fertility. However, the functions and mechanisms of miR-214-3p on granulosa cells are unclear. In this study, miR-214-3p was transfected into porcine ovarian granulosa cells to investigate its functions in terms of proliferation and estradiol synthesis via flow cytometry, CCK-8 assay, EdU staining, ElisA, Real-Time PCR, and Western blot analyses. We also identified the targets of miR-214-3p via Luciferase Reporter Assay. Results: Our findings revealed that miR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine granulosa cells. We also found that miR-214-3p up-regulates the expression of cell cycle genes including Cell cycle protein B (Cyclin B), Cell cycle protein D (Cyclin D), Cell cycle protein E (Cyclin E), and Cyclin-dependent kinase 4 (CDK4) at the transcription and translation levels, while down-regulating the mRNA and protein levels of cytochrome P450 family 11 subfamily A member 1 (CYP11A1), cytochrome P450 family 19 subfamily A member 1 (CYP19A1), and steroidogenic acute regulatory protein (StAR) (i.e., the key enzymes in estradiol synthesis). On-line prediction, bioinformatics analysis, a luciferase reporter assay, RT-qPCR, and Western blot results showed that the target genes of miR-214-3p in proliferation and estradiol synthesis are Mfn2 and NR5A1, respectively. Conclusions: Our findings suggest that miR-214-3p plays an important role in the functional regulation of porcine granulosa cells and therefore may be a target gene for regulating follicular development.

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Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2

Cited by 23 publications

References 49 publications

A Preliminary Study on the Characteristics of microRNAs in Ovarian Stroma and Follicles of Chuanzhong Black Goat during Estrus

A Preliminary Study on the Characteristics of microRNAs in Ovarian Stroma and Follicles of Chuanzhong Black Goat during Estrus

MiR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine granulosa cells

MiR-214-3p Promotes Proliferation and Inhibits Estradiol Synthesis In Porcine Granulosa Cells

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