Trans-specific S-RNase and SFB alleles in Prunus self-incompatibility haplotypes

Abstract

Self-incompatibility in the genus Prunus is controlled by two genes at the S-locus, S-RNase and SFB. Both genes exhibit the high polymorphism and high sequence diversity characteristic of plant self-incompatibility systems. Deduced polypeptide sequences of three myrobalan and three domestic plum S-RNases showed over 97% identity with S-RNases from other Prunus species, including almond, sweet cherry, Japanese apricot and Japanese plum. The second intron, which is generally highly polymorphic between alleles was also remarkably well conserved within these S-allele pairs. Degenerate consensus primers were developed and used to amplify and sequence the co-adapted polymorphic SFB alleles. Sequence comparisons also indicated high degrees of polypeptide sequence identity between three myrobalan and the three domestic plum SFB alleles and the corresponding Prunus SFB alleles. We discuss these trans-specific allele identities in terms of S-allele function, evolution of new allele specificities and Prunus taxonomy and speciation.

Functional gametophytic self-incompatibility in a peripheral population of Solanum peruvianum (Solanaceae)

“…(Lu, 2001;Savage and Miller, 2006;Surbanovski et al, 2007;Vieira et al, 2007;Sutherland et al, 2008). Similar allele pairs across species could be a result of recently shared evolutionary history (Lu, 2001;Sutherland et al, 2008) or introgression among species (for example, Castric et al, 2008).…”

“…(Lu, 2001;Savage and Miller, 2006;Surbanovski et al, 2007;Vieira et al, 2007;Sutherland et al, 2008). Similar allele pairs across species could be a result of recently shared evolutionary history (Lu, 2001;Sutherland et al, 2008) or introgression among species (for example, Castric et al, 2008). Solanum peruvianum and S. chilense are certainly recently diverged (p0.55 million years, Städler et al, 2008), and analyses of multilocus sequence data suggest historical introgression (Städler et al, 2005(Städler et al, , 2008.…”

Prunus

“…7.3 illustrate several striking and related features that have been noted in recent studies of the evolution of S-RNase and SFB genes in Prunus (Nunes et al 2006;Sutherland et al 2008;Tsukamoto et al 2008;Vieira et al 2008). First, the two phylogenies have some similar characteristics: homoplasy is high (consistency indices are low) in both data sets, support for the relationships among lineages of both genes, especially the deeper internal branches, is generally weak, and many of those branches are quite short, suggesting that early diversification of these genes may have occurred rapidly in ancestral species, with subsequent lineage sorting and/or recombination giving rise to the extant alleles, as suggested by Tsukamoto et al (2008).…”

The myrobalan (Prunus cerasifera L.): a useful diploid model for studying the molecular genetics of self-incompatibility in plums

“…Genomic PCR with second intron consensus primers DNA from each of the 25 P. cerasifera accessions was ampliWed in PCR with consensus primers designed to span the polymorphic second intron of Prunus S-RNase and to hybridize with C1, C2, and C5 conserved regions, respectively: EM-PC1consFD (Sutherland et al 2008) and EM-PC2consFD in the forward orientation and EM-PC5consRD in the reverse (Sutherland et al 2004). The PCR reaction components and conditions were identical for both primer combinations, and are described in Sutherland et al (2004).…”