Trans-species variation in<i>Dmrt1</i>is associated with sex determination in four European tree-frog species

Brelsford, Alan; Dufresnes, Christophe; Perrin, Nicolas

doi:10.1111/evo.12891

Cited by 30 publications

(30 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Europe, Dmrt1is either sex-linked or likely important in sexual differentiation in several Hylidae, Rana temporaria , and Bufo viridis [43, 44]. Furthermore, Dmrt1is an important gene in sexual differentiation in the Japanese wrinkled frog ( Rana rugosa ), despite being autosomal [45].…”

Section: Discussionmentioning

confidence: 99%

“…Brelsford et al [26] suggested sampling from a broader geographic distribution and with more genetic variation may be useful for identifying sex-linked loci. Furthermore, Brelsford et al [44] used a GBS approach on four species of European Hylid frogs. With four males and four females of each species, they found between 17 and 54 putative sex-linked SNP loci for each species out of between 4043 and 10,211 possible polymorphic sites [41].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Sex-linked markers in the North American green frog (Rana clamitans) developed using DArTseq provide early insight into sex chromosome evolution

2016

View full text Add to dashboard Cite

BackgroundThe extent to which sex reversal is associated with transitions in sex determining systems (XX-XY, ZZ-ZW, etc.) or abnormal sexual differentiation is predominantly unexplored in amphibians. This is in large part because most amphibian taxa have homomorphic sex chromosomes, which has traditionally made it challenging to identify discordance between phenotypic and genetic sex in amphibians, despite all amphibians having a genetic component to sex determination. Recent advances in molecular techniques such as genome complexity reduction and high throughput sequencing present a valuable avenue for furthering our understanding of sex determination in amphibians and other taxa with homomorphic sex chromosomes like many fish and reptiles.ResultsWe use DArTseq as a novel approach to identify sex-linked markers in the North American green frog (Rana clamitans melanota) using lab-reared tadpoles as well as wild-caught adults from seven ponds either in undeveloped, forested habitats or suburban ponds known to be subject to contamination by anthropogenic chemicals. The DArTseq methodology identified 13 sex-linked SNP loci and eight presence-absence loci associated with males, indicating an XX-XY system. Both alleles from a single locus show partial high sequence homology to Dmrt1, a gene linked to sex determination and differentiation throughout Metazoa. Two other loci have sequence similarities to regions of the chimpanzee and human X-chromosome as well as the chicken Z-chromosome. Several loci also show geographic variation in sex-linkage, possibly indicating sex chromosome recombination. While all loci are statistically sex-linked, they show varying degrees of female heterozygosity and male homozygosity, providing further evidence that some markers are on regions of the sex chromosomes undergoing higher rates of recombination and therefore further apart from the putative sex determining locus.ConclusionThe ease of the DArTseq platform provides a useful avenue for future research on sex reversal and sex chromosome evolution in vertebrates, particularly for non-model species with homomorphic or cryptic or nascent sex chromosomes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3209-x) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Sex-linked markers in the North American green frog (Rana clamitans) developed using DArTseq provide early insight into sex chromosome evolution

2016

View full text Add to dashboard Cite

show abstract

“…2 ; Table 1 ). In particular, chromosome 1 is a highly potential candidate sex chromosome in anurans as it is reported to be a sex chromosome in some species of other genera, such as Hyla and Bufo [Stöck et al, 2013;Brelsford et al, 2016]. To date, sex-determining genes on the 6 sex chromosomes remain unidentified, although 3 sex-determining genes identified in vertebrates are mapped in G. rugosa : Dmrt1 and Amh on chromosome 1 and SOX3 on chromosome 7 Kodama et al, 2015].…”

mentioning

confidence: 99%

Sex Determination and Sex Chromosomes in Amphibia

Miura

2017

Sex Dev

113

View full text Add to dashboard Cite

Sex chromosomes in most amphibians are homomorphic (undifferentiated) in both sexes and are characterized by frequent turnover. This is in sharp contrast to sex chromosomes in 2 major vertebrate groups, the mammals and birds, where they are heteromorphic in one sex and are highly conserved. Sex-determining mechanisms in anuran amphibians, particularly in relation to the turnover of sex-determining genes and sex chromosomes, are summarized and their evolution is discussed.

show abstract

“…) and is identified as a strong candidate sex‐determining gene in several groups of frogs (Brelsford et al. ), including R. temporaria .…”

Section: Y‐ and X‐specific Dmrt Haplotypesmentioning

confidence: 99%

Phylogeography, more than elevation, accounts for sex chromosome differentiation in Swiss populations of the common frog ( Rana temporaria )*

et al. 2019

Self Cite

View full text Add to dashboard Cite

Sex chromosomes in vertebrates range from highly heteromorphic (as in most birds and mammals) to strictly homomorphic (as in many fishes, amphibians, and nonavian reptiles). Reasons for these contrasted evolutionary trajectories remain unclear, but species such as common frogs with polymorphism in the extent of sex chromosome differentiation may potentially deliver important clues. By investigating 92 common frog populations from a wide range of elevations throughout Switzerland, we show that sex chromosome differentiation strongly correlates with alleles at the candidate sex‐determining gene Dmrt1. Y‐specific Dmrt1 haplotypes cluster into two main haplogroups, YA and YB, with a phylogeographic signal that parallels mtDNA haplotypes: YA populations, with mostly well‐differentiated sex chromosomes, occur primarily south of the main alpine ridge that bisects Switzerland, whereas YB populations, with mostly undifferentiated (proto‐)sex chromosomes, occur north of this ridge. Elevation has only a marginal effect, opposing previous suggestions of a major role for climate on sex chromosome differentiation. The Y‐haplotype effect might result from differences in the penetrance of alleles at the sex‐determining locus (such that sex reversal and ensuing X‐Y recombination are more frequent in YB populations), and/or fixation of an inversion on YA (as supported by the empirical observation that YA haplotypes might not recombine in XYA females).

show abstract

Trans-species variation inDmrt1is associated with sex determination in four European tree-frog species

Cited by 30 publications

References 41 publications

Sex-linked markers in the North American green frog (Rana clamitans) developed using DArTseq provide early insight into sex chromosome evolution

Sex-linked markers in the North American green frog (Rana clamitans) developed using DArTseq provide early insight into sex chromosome evolution

Sex Determination and Sex Chromosomes in Amphibia

Phylogeography, more than elevation, accounts for sex chromosome differentiation in Swiss populations of the common frog ( Rana temporaria )*

Contact Info

Product

Resources

About