trans-Resveratrol Ameliorates Stress-Induced Irritable Bowel Syndrome-Like Behaviors by Regulation of Brain-Gut Axis

Abstract

Background: Irritable bowel syndrome (IBS) is a functional disorder characterized by abdominal pain and abnormalities in defecation associated with psychiatric disorders such as depression and anxiety due to the dysfunction of brain-gut axis. This study aims to determine whether trans-Resveratrol affects chronic-acute combined stress (CACS)-induced IBS-like symptoms including depression, anxiety and intestinal dysfunction.Methods: ICR male mice were exposed to the CACS for 3 weeks. trans-Resveratrol were administrated daily (2.5, 5, and 10 mg/kg, i.g.) 30 min before CACS. Behavioral tests were performed to evaluate the treatment effects of trans-Resveratrol on IBS. Hippocampus tissues were collected and processed Golgi staining and immuno-blot analysis. Ileum and colon tissues were collected and processed Hematoxylin and Eosin staining and immuno-blot analysis.Results: Administration with trans-Resveratrol before CACS for 3 weeks significantly reversed CACS-induced depression- and anxiety-like behaviors and intestinal dysfunction in mice, which implied a crucial role of trans-Resveratrol in treatment of IBS-like disorder. Furthermore, trans-Resveratrol improved hippocampal neuronal remodeling, protected ileal and colonic epithelial barrier structure against CACS insults. The further study suggested that trans-Resveratrol normalized phosphodiesterases 4A (PDE4A) expression and CREB-BDNF signaling that were disturbed by CACS. The increased pCREB and BDNF expression in the hippocampus were found, while decreased pCREB and BDNF levels were observed after treatment with trans-Resveratrol.Conclusions: The dual effects of trans-Resveratrol on stress-induced psychiatric and intestinal dysfunction may be related to normalization of PDE4A expression and subsequent pCREB-BDNF signaling in the hippocampus, ileum and colon.

Differentially Expressed Genes in the Brain of Aging Mice With Cognitive Alteration and Depression- and Anxiety-Like Behaviors

“…Morris water maze (MWM) was another approach to evaluate spatial learning in rodents, as described previously (Vorhees and Williams, 2006;Xu et al, 2018). Mice were trained in a water maze (120 cm in diameter, 50 cm in depth) filled with water (25 cm in depth,23 ± 0.5 • C).…”

“…Paw withdrawal frequencies in response to mechanical stimuli were tested as described before (Xu et al, 2018). Briefly, each mouse was placed individually in a Plexiglas chamber on an elevated mesh.…”

Transcriptome Analysis of Juvenile Tilapia (Oreochromis niloticus) Blood, Fed With Different Concentrations of Resveratrol

“…In the comparison between hepatic (previous data) and blood transcriptomes, 89 shared DEGs were enriched in antigen processing and presentation (Bonifaz et al, 2015;Matheoud et al, 2016), cell adhesion molecules (associated with phosphorylation and apoptosis (Gledhill et al, 2007;Lin et al, 2009) via the Sirt1-PGC1α-PPARα pathway (Milton-Laskibar et al, 2018), and the phagosome (Rubinsztein et al, 2011) pathways. However, 3479 and 747 DEGs were enriched as specifically expressed genes in liver and blood, and it hinted that RES aroused different response in blood (differ from liver), but it may exist some crosstalk based on the shared pathways (like circulation axis, Xu et al, 2018;Zhou et al, 2019). Actually no reports focused on the pathological features of Alzheimer's Disease in fish, but a lot of studies used zebrafish as the model for discussing how to care with neurological disorders, or nervous system diseases.…”

<p>Influence Of Narrative Medicine-Based Health Education Combined With An Online Patient Mutual Assistance Group On The Health Of Patients With Inflammatory Bowel Disease and Arthritis</p>

“…On the other hand, narrative medicine has shown to affect the physical symptoms of patients by affecting their psychological state. 35 The relationship between psychology and digestive tract symptoms is mainly through the brain-gut axis. The brain-gut axis is bidirectionally regulated.…”