Trans-omics approaches used to characterise fish nutritional biorhythms in leopard coral grouper (Plectropomus leopardus)

Abstract

Aquaculture is now a major supplier of fish, and has the potential to be a major source of protein in the future. Leopard coral groupers are traded in Asian markets as superior fish, and production via aquaculture has commenced. As feeding efficiency is of great concern in aquaculture, we sought to examine the metabolism of leopard coral groupers using trans-omics approaches. Metabolic mechanisms were comprehensively analysed using transcriptomic and metabolomic techniques. This study focused on the dynamics of muscular metabolites and gene expression. The omics data were discussed in light of circadian rhythms and fasting/feeding. The obtained data suggest that branched-chain amino acids played a role in energy generation in the fish muscle tissues during fasting. Moreover, glycolysis, TCA cycles, and purine metabolic substances exhibited circadian patterns, and gene expression also varied. This study is the first step to understanding the metabolic mechanisms of the leopard coral grouper.

Metabolomics and fish nutrition: a review in the context of sustainable feed development

“…Therefore, metabolomics can be the entry point to understand metabolic functions, and its combination with other omic approaches should foster confidence in the results. Mekuchi et al (2017) have, for example, used transcriptomics and metabolomics to assess the influence of circadian rhythm on nutritional state of leopard coral grouper (Plectropomus leopardus).…”

“…The effect of food deprivation combined with the influence of circadian rhythm led to an increase in leucine and isoleucine amino acids as a potential signal at cell level to regulate energy metabolism (Mekuchi et al . ). Such energetic metabolites variations are also observed in fish fed with diets in which marine resources are replaced by plant products, suggesting that energy status become deficient in these fish.…”

“…As revealed by GC-MS, depleted levels of oxalate and malonate, which are inhibitors of specific TCA cycle enzymes, also suggest an alteration in energy status in fasted fish (Baumgarner & Cooper 2012). The effect of food deprivation combined with the influence of circadian rhythm led to an increase in leucine and isoleucine amino acids as a potential signal at cell level to regulate energy metabolism (Mekuchi et al 2017). Such energetic metabolites variations are also observed in fish fed with diets in which marine resources are replaced by plant products, suggesting that energy status become deficient in these fish.…”

“…Mekuchi et al . () have, for example, used transcriptomics and metabolomics to assess the influence of circadian rhythm on nutritional state of leopard coral grouper ( Plectropomus leopardus ).…”

Regional feature extraction of various fishes based on chemical and microbial variable selection using machine learning

“…We have previously proposed that the host chemical 39 and gut microbial proles of sh are strongly inuenced by their food source, 22 suggesting that these host and symbiotic proles could provide insights into the habitats the samples were collected from. From this perspective, we have developed visualization methods to examine the chemical and microbial correlations in aquatic environments (e.g.…”