Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K

The pKNOCK Series of Broad-Host-Range Mobilizable Suicide Vectors for Gene Knockout and Targeted DNA Insertion into the Chromosome of Gram-Negative Bacteria

“…Upon stimulation of the Fas receptor, FADD and the cytosolic domain of Fas receptor interact through a domain known as death domain (DD), located in the C terminus of both proteins. Simultaneously, the FADD N-terminal region, termed death effector domain (DED), interacts with at least one member of the caspase family, caspase-8 (FLICE, MACH, Mch5), linking it to Fas receptor (2,3,5,8). The FADD gene comprises 2 exons, 1 and 2, which code for DED and DD domains, respectively (7).…”

Preponderance of Fis-binding sites in the R6K gamma origin and the curious effect of the penicillin resistance marker on replication of this origin in the absence of Fis

“…The enhancer contains DnaA box 1 (79, 80) and a small segment, stb, involved in stable maintenance of ␥-origin-containing plasmids (81). We subdivided the core into three distinct regions (16): (i) the AT-rich region, bound by and IHF (11,13,15,17,19,45,52); (ii) seven 22-bp direct repeats (DRs) bound by (19,23,28,60); and (iii) a multiprotein-binding region interacting with DnaA, IHF, and RNA polymerase (11,13,15,32,77,79).Replication from each R6K origin requires the R6K initiator protein , encoded by the pir gene (47,48,(70)(71)(72). This gene is required in cis for activation of the ␤ origin (71, 72) but can be provided in cis or trans to regulate activities of the ␥ and ␣ origins (47,48,70).…”

“…Replication from each R6K origin requires the R6K initiator protein , encoded by the pir gene (47,48,(70)(71)(72). This gene is required in cis for activation of the ␤ origin (71, 72) but can be provided in cis or trans to regulate activities of the ␥ and ␣ origins (47,48,70).…”

“…This gene is required in cis for activation of the ␤ origin (71, 72) but can be provided in cis or trans to regulate activities of the ␥ and ␣ origins (47,48,70). When provided at a low intracellular level, activates R6K replication, but high levels inhibit replication (20).…”

“…1). Each origin requires in cis the 277-bp core segment and an additional segment unique to that origin (47,48,(70)(71)(72). The ␥ origin, for example, includes the core and the adjacent enhancer (for a recent review, see reference 16).…”