Tralopyril induces developmental toxicity in zebrafish embryo (Danio rerio) by disrupting the thyroid system and metabolism

Chen, Xiangguang; Teng, Miaomiao; Zhang, Jie; Qian, Le; Duan, Manman; Cheng, Yi; Zhao, Feng; Wang, Chengju

doi:10.1016/j.scitotenv.2020.141860

Cited by 35 publications

(10 citation statements)

References 57 publications

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“…The significant increase in citric acid showed that chlorfenapyr affected the tricarboxylic acid cycle. Creatine is an amino acid derivative that helps provide energy to muscles and nerve cells: when an organism lacks ATP, creatine can quickly resynthesize ATP to supply energy . In the current study, the changes to creatine are similar to the effect of tralopyril on zebrafish larvae, verifying that chlorfenapyr was metabolized into tralopyril in zebrafish.…”

Section: Discussionsupporting

confidence: 69%

“…55 Therefore, the change of phenylalanine content indicated that chlorfenapyr may also disturb thyroid hormone homeostasis in zebrafish, just as tralopyril disrupted thyroid hormone levels in zebrafish larvae. 46 Depuration. After 9 days of depuration following chronic chemical exposure, the expression level of genes related to oxidative damage, apoptosis, and immunity, as well as enzyme activity, returned to normal, but serious lesions in the liver and brain were still observed.…”

Section: Quantification Of Chlorfenapyr and Tralopyril Inmentioning

confidence: 99%

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Bioaccumulation, Metabolism and the Toxic Effects of Chlorfenapyr in Zebrafish (Danio rerio)

Chen

Teng

Zhang

et al. 2021

J. Agric. Food Chem.

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Chlorfenapyr is widely used as an insecticide/miticide. Tralopyril, the active metabolite of chlorfenapyr, is used as an antifouling biocide in antifouling systems, and negatively affects aquatic environments. However, it is unclear whether tralopyril is a metabolite of chlorfenapyr in aquatic vertebrates, and there is little data on the bioaccumulation and toxicity of chlorfenapyr to aquatic vertebrates. In this study, the bioaccumulation and elimination of chlorfenapyr in zebrafish were assessed, and tralopyril, the active metabolite of chlorfenapyr, was determined. The effects of chronic exposure to chlorfenapyr on zebrafish liver and brain oxidative damage, apoptosis, immune response, and metabolome were investigated. These results showed that chlorfenapyr has a high bioaccumulation in zebrafish, with bioaccumulation factors of 864.6 and 1321.9 after exposure to 1.0 and 10 μg/L chlorfenapyr for 21 days, respectively. Chlorfenapyr at these concentrations also rapidly accumulated in zebrafish, reaching 615.5 and 10336 μg/kg on the second and third days of exposure, respectively. Chlorfenapyr was degraded to tralopyril in zebrafish; therefore, both chlorfenapyr and tralopyril should be considered when evaluating the risk of chlorfenapyr to aquatic organisms. In addition, chronic exposure caused oxidative damage, apoptosis, and immune disorders in zebrafish liver. Chronic exposure also altered the levels of endogenous metabolites in liver and brain. After 9 days of depuration, some indicators of oxidative damage, apoptosis, and immunity returned to normal levels, but the concentration of endogenous metabolites in zebrafish liver was still altered. Overall, these results provide useful information for evaluating the toxicity and environmental fate of chlorfenapyr in aquatic vertebrates.

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Section: Discussionsupporting

confidence: 69%

Section: Quantification Of Chlorfenapyr and Tralopyril Inmentioning

confidence: 99%

Bioaccumulation, Metabolism and the Toxic Effects of Chlorfenapyr in Zebrafish (Danio rerio)

Chen

Teng

Zhang

et al. 2021

J. Agric. Food Chem.

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“…The authors used a combination of 1 H-NMR for the analysis of monosaccharides, amino acids, and organic acids, GC-MS for cholesterol and fatty acids, and LC-MS for the analysis of lipids. However, more recent zebrafish toxicometabolomics studies (≥2017) reported using LC-HRMS (Orbitrap or QTOF) [15,28,29,31,33,36,48,51,[85][86][87][88], followed by 1 H-NMR [24,30,[89][90][91][92][93] and GC-HRMS [21,94], while multi-analytical platform studies remain scarce.…”

Section: Instrumental Analysismentioning

confidence: 99%

Optimization of a liquid chromatography-ion mobility-high resolution mass spectrometry platform for untargeted lipidomics and application to HepaRG cell extracts

Silva

Iturrospe

Heyrman

et al. 2021

Talanta

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“…The authors used a combination of 1 H-NMR for the analysis of monosaccharides, amino acids, and organic acids, GC-MS for cholesterol and fatty acids, and LC-MS for the analysis of lipids. However, more recent zebrafish toxicometabolomics studies (≥2017) reported using LC-HRMS (Orbitrap or QTOF) [ 15 , 28 , 29 , 31 , 33 , 36 , 48 , 51 , 85 , 86 , 87 , 88 ], followed by 1 H-NMR [ 24 , 30 , 89 , 90 , 91 , 92 , 93 ] and GC-HRMS [ 21 , 94 ], while multi-analytical platform studies remain scarce.…”

Section: Experimental Designmentioning

confidence: 99%

Mass Spectrometry-Based Zebrafish Toxicometabolomics: A Review of Analytical and Data Quality Challenges

et al. 2021

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Metabolomics has achieved great progress over the last 20 years, and it is currently considered a mature research field. As a result, the number of applications in toxicology, biomarker, and drug discovery has also increased. Toxicometabolomics has emerged as a powerful strategy to provide complementary information to study molecular-level toxic effects, which can be combined with a wide range of toxicological assessments and models. The zebrafish model has gained importance in recent decades as a bridging tool between in vitro assays and mammalian in vivo studies in the field of toxicology. Furthermore, as this vertebrate model is a low-cost system and features highly conserved metabolic pathways found in humans and mammalian models, it is a promising tool for toxicometabolomics. This short review aims to introduce zebrafish researchers interested in understanding the effects of chemical exposure using metabolomics to the challenges and possibilities of the field, with a special focus on toxicometabolomics-based mass spectrometry. The overall goal is to provide insights into analytical strategies to generate and identify high-quality metabolomic experiments focusing on quality management systems (QMS) and the importance of data reporting and sharing.

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Tralopyril induces developmental toxicity in zebrafish embryo (Danio rerio) by disrupting the thyroid system and metabolism

Cited by 35 publications

References 57 publications

Bioaccumulation, Metabolism and the Toxic Effects of Chlorfenapyr in Zebrafish (Danio rerio)

Bioaccumulation, Metabolism and the Toxic Effects of Chlorfenapyr in Zebrafish (Danio rerio)

Optimization of a liquid chromatography-ion mobility-high resolution mass spectrometry platform for untargeted lipidomics and application to HepaRG cell extracts

Mass Spectrometry-Based Zebrafish Toxicometabolomics: A Review of Analytical and Data Quality Challenges

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