“…We assessed general pathways enriched in NRVM and iPSC‐CM by gene ontology, which further supports our findings that iPSC‐CM are in a maturation pseudotime between P7 and P14 CM, whereas NRVM are in P0 pseudotime, with ensuing distinct transcriptomic profiles. Our results are corroborated by a recent single‐cell study characterizing the maturation of iPSC‐CM and P0‐P84 CM 74 that also suggested an overlap between iPSC‐CM and P11‐P14 CM.…”
Anthracyclines such as doxorubicin (Dox) are effective chemotherapeutic agents; however, their use is hampered by subsequent cardiotoxicity risk. Our understanding of cardiomyocyte protective pathways activated following anthracyclineinduced cardiotoxicity (AIC) remains incomplete. Insulin-like growth factor binding protein (IGFBP) 3 (Igfbp-3), the most abundant IGFBP family member in the circulation, is associated with effects on the metabolism, proliferation, and survival of various cells. Whereas Igfbp-3 is induced by Dox in the heart, its role in AIC is ill-defined. We investigated molecular mechanisms as well as systemslevel transcriptomic consequences of manipulating Igfbp-3 in AIC using neonatal rat ventricular myocytes and human-induced pluripotent stem cell-derived cardiomyocytes. Our findings reveal that Dox induces the nuclear enrichment of
“…We assessed general pathways enriched in NRVM and iPSC‐CM by gene ontology, which further supports our findings that iPSC‐CM are in a maturation pseudotime between P7 and P14 CM, whereas NRVM are in P0 pseudotime, with ensuing distinct transcriptomic profiles. Our results are corroborated by a recent single‐cell study characterizing the maturation of iPSC‐CM and P0‐P84 CM 74 that also suggested an overlap between iPSC‐CM and P11‐P14 CM.…”
Anthracyclines such as doxorubicin (Dox) are effective chemotherapeutic agents; however, their use is hampered by subsequent cardiotoxicity risk. Our understanding of cardiomyocyte protective pathways activated following anthracyclineinduced cardiotoxicity (AIC) remains incomplete. Insulin-like growth factor binding protein (IGFBP) 3 (Igfbp-3), the most abundant IGFBP family member in the circulation, is associated with effects on the metabolism, proliferation, and survival of various cells. Whereas Igfbp-3 is induced by Dox in the heart, its role in AIC is ill-defined. We investigated molecular mechanisms as well as systemslevel transcriptomic consequences of manipulating Igfbp-3 in AIC using neonatal rat ventricular myocytes and human-induced pluripotent stem cell-derived cardiomyocytes. Our findings reveal that Dox induces the nuclear enrichment of
“…We also published an extended in vivo cardiomyocyte reference from embryonic day 14 (e14) to postnatal day 84 (p84). 34 > load("clean_060720.RData") …”
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