Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens.

O'Sullivan, Dan; O’Gara, Fergal

doi:10.1128/mmbr.56.4.662-676.1992

Cited by 433 publications

(156 citation statements)

References 100 publications

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“…against several fungal pathogens, including R. solani. The inhibition of R. solani by bacteria may be due to the production of antibiotics or siderophores or both (O'Sullivan & O'Gara, 1992). In the present study, we found that 16 of the 57 strongly antagonistic isolates produced fluorescent siderophores and that they belonged to the group fluorescent Pseudomonas.…”

Section: Discussionsupporting

confidence: 48%

Evidence for specificity of cultivable bacteria associated with arbuscular mycorrhizal fungal spores

Bharadwaj

Lundquist

Persson

et al. 2008

FEMS Microbiology Ecology

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Bacteria associated with arbuscular mycorrhizal (AM) fungal spores may play functional roles in interactions between AM fungi, plant hosts and defence against plant pathogens. To study AM fungal spore-associated bacteria (AMB) with regard to diversity, source effects (AM fungal species, plant host) and antagonistic properties, we isolated AMB from surface-decontaminated spores of Glomus intraradices and Glomus mosseae extracted from field rhizospheres of Festuca ovina and Leucanthemum vulgare. Analysis of 385 AMB was carried out by fatty acid methyl ester (FAME) profile analysis, and some also identified using 16S rRNA gene sequence analysis. The AMB were tested for capacity to inhibit growth in vitro of Rhizoctonia solani and production of fluorescent siderophores. Half of the AMB isolates could be identified to species (similarity index 0.6) within 16 genera and 36 species. AMB were most abundant in the genera Arthrobacter and Pseudomonas and in a cluster of unidentified isolates related to Stenotrophomonas. The AMB composition was affected by AM fungal species and to some extent by plant species. The occurrence of antagonistic isolates depended on AM fungal species, but not plant host, and originated from G. intraradices spores. AM fungal spores appear to host certain sets of AMB, of which some can contribute to resistance by AM fungi against plant pathogens.

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Section: Discussionsupporting

confidence: 48%

Evidence for specificity of cultivable bacteria associated with arbuscular mycorrhizal fungal spores

Bharadwaj

Lundquist

Persson

et al. 2008

FEMS Microbiology Ecology

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“…Similarly, endophytes can also promote plant growth in an attempt to outcompete cell apoptosis induced by infecting pathogens (Berg and Hallmann, 2006). Plant growth promotion by endophytes may be exerted by several mechanisms, such as production of phytohormones (Tudzynski, 1997), synthesis of siderophores (O'Sullivan and O'Gara, 1992), nitrogen fixation, solubilisation of minerals such as phosphorus (Richardson et al, 2009), or via enzymatic activities, such as ethylene suppression by 1-aminocyclopropane-1-carboxylate deaminase (Glick et al, 1998). The plant host also benefits from the endophytes by their natural resistance to soil contaminants (Siciliano et al, 2001), their ability to degrade xenobiotics, or their action as vectors to introduce degradative traits to plants, which substantially assist phytoremediation (Ryan et al, 2008).…”

Section: Endophytes As Sources Of Natural Productsmentioning

confidence: 99%

Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds

Alvin

Miller

Neilan

2014

Microbiological Research

317

153

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Natural product drug discovery has regained interest due to low production costs, structural diversity, and multiple uses of active compounds to treat various diseases. Attention has been directed towards medicinal plants as these plants have been traditionally used for generations to treat symptoms of numerous diseases. It is established that plants harbour microorganisms, collectively known as endophytes. Exploring the as-yet untapped natural products from the endophytes increases the chances of finding novel compounds. The concept of natural products targeting microbial pathogens has been applied to isolate novel antimycobacterial compounds, and the rapid development of drug-resistant Mycobacterium tuberculosis has significantly increased the need for new treatments against this pathogen. It remains important to continuously screen for novel compounds from natural sources, particularly from rarely encountered microorganisms, such as the endophytes. This review focuses on bioprospecting for polyketides and small peptides exhibiting antituberculosis activity, although current treatments against tuberculosis are described. It is established that natural products from these structure classes are often biosynthesised by microorganisms. Therefore it is hypothesised that some bioactive polyketides and peptides originally isolated from plants are in fact produced by their endophytes. This is of interest for further endophyte natural product investigations.

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“…For several antibiotics produced by strains of di¡erent bacterial genera, biosynthetic genes have been cloned and partially or fully sequenced. These include zwittermycin A produced by B. cereus [4], AFC-BC11 produced by B. cepacia [5] and the antibiotics 2,4diacetylphloroglucinol (2,, phenazines (PHZs), pyrrolnitrin (PRN), and pyoluteorin (PLT) produced by various Pseudomonas species [6^11]. A prerequisite for DNA-based detection and isolation is that the genes of interest must be conserved among a wide range of bacterial strains harboring this trait.…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms within the prnD and pltC genes from pyrrolnitrin and pyoluteorin-producing Pseudomonas and Burkholderia spp.

Souza¹,

Raaijmakers²

2003

FEMS Microbiology Ecology

124

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Pyrrolnitrin (PRN) and pyoluteorin (PLT) are broad-spectrum antibiotics produced by several strains of Pseudomonas and Burkholderia species. Both antibiotics play an important role in the suppression of multiple plant pathogenic fungi. Primers were developed from conserved sequences and amplified prnD and pltC fragments from 18 Pseudomonas and four Burkholderia spp. of worldwide origin that produce either PRN or PLT or both. Subsequent RFLP (restriction fragment length polymorphisms) analysis of the 438-bp pltC fragment showed no polymorphisms among PLT-producing Pseudomonas strains. Polymorphisms within the 786-bp prnD fragment, however, allowed the assessment of the diversity among PRN-producing Pseudomonas and Burkholderia spp. to a level similar to that obtained by three 10-mer primers in random amplified polymorphic DNA analysis. Phylogenetic analysis of 16S rDNA sequences of strains representative of PRN-producing Pseudomonas and Burkholderia species correlated well with their taxonomic status. Phylogenetic relationships inferred from each of the four prn genes and from the complete sequence of the prn biosynthetic locus were similar to 16S rDNA-based phylogeny for most strains, except for Burkholderia pyrrocinia DSM 10685. Both RFLP analysis and comparison of the prn gene sequences showed that B. pyrrocinia DSM 10685 was more closely related to PRN-producing Pseudomonas strains, suggesting that lateral gene transfer may have occurred. Colony hybridization and PCR with PRN- and PLT-specific probes and primers showed that Pseudomonas and Burkholderia spp. harboring the prnD and pltC gene were not present at detectable levels on roots of wheat grown in five agricultural soils collected in The Netherlands, two of them being naturally suppressive to Gaeumannomyces graminis var. tritici. These results suggest that PRN- and PLT-producing Pseudomonas and Burkholderia sp. do not contribute to the natural suppressiveness found in these Dutch take-all decline soils.

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Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens.

Cited by 433 publications

References 100 publications

Evidence for specificity of cultivable bacteria associated with arbuscular mycorrhizal fungal spores

Evidence for specificity of cultivable bacteria associated with arbuscular mycorrhizal fungal spores

Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds

Polymorphisms within the prnD and pltC genes from pyrrolnitrin and pyoluteorin-producing Pseudomonas and Burkholderia spp.

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