Traitor-virus-guided discovery of novel antiviral factors
Caterina Prelli Bozzo,
Alexandre Laliberté,
Aurora De Luna
et al.
Abstract:Complex pathogen-host interactions govern the outcome of viral exposures but remain poorly understood because current methods to elucidate antiviral mechanisms are prone to artefacts and lack sensitivity. Here, we developed a virus-guided technology platform where the pathogen itself reveals its cellular opponents. To accomplish this, we engineered replication-competent HIV-1 expressing sgRNAs targeting potential antiviral genes in Cas9-expressing CD4+T cells. Simultaneous analysis of HIV-1 constructs targetin… Show more
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