Trait Variation between Two Wild Specimens of Pleurotus ostreatus and Their Progeny in the Context of Usefulness in Nematode Control

Abstract

Pleurotus ostreatus is one of the most widespread mushrooms in the world. It is a valuable and widely cultivated edible mushroom with nematicidal properties. The mycelium of this mushroom produces a toxin that paralyzes nematodes, after which the nematode becomes infected with the filament, resulting in its death. This feature can be put to practical use as a natural nematicidal agent. In this paper, we have described studies on two wild strains of P. ostreatus (PO1, PO2) and the monokaryotic progeny obtained from their fruiting bodies. Monokaryons were crossed with each other within the progeny of one strain (PO1xPO1; PO2xPO2) and of two different strains (PO2xPO1). The growth conditions, morphological and molecular characteristics, toxicity and ability to form hyphal knobs of the maternal strains, monokaryons and those obtained by crossing were studied. The toxicity of mycelium was tested against the model organism Caenorhabditis elegans. The results of the study allowed the selection of five progeny heterokaryotic mycelia with the best parameters useful for in-vitro control of nematodes.

An Approach For Control Of Phytopathogenic Caenorhabditis elegans N2 Via Regulation of Growth Conditions And Pleurotus ostreatus Po4

“…For example, the use of polyphenolic compounds from olive mill waste waters 32 or biosynthesized silver nanoparticles 33 as well as activity of bacteria (Lysobacter capsici, Bacillus cereus, B. thuringiensis, Pseudomonas chlororaphis, Serratia proteamaculans, etc. 31 ) and fungi (Arthrobotrys oligospora, Drechslerella dactyloides 34 , P. ostreatus 7,11,12 , etc.) are considered to be promising for nematodes control.…”

“…The mechanisms of nematodes' control are still unclear. However, P. ostreatus is considered to be the one of the most promising to control nematodes [10][11][12] .…”

An Approach for the Control of Caenorhabditis elegans N2 via the Regulation of Growth Conditions and Pleurotus ostreatus Po4

“…For example, the use of polyphenolic compounds from olive mill waste waters [32] or biosynthesized silver nanoparticles [33] as well as the activities of bacteria (Lysobacter capsici, Bacillus cereus, B. thuringiensis, Pseudomonas chlororaphis, Serratia proteamaculans, etc. [31]) and fungi [34] (Arthrobotrys oligospora, Drechslerella dactyloides [35], P. ostreatus [8,[12][13][14], etc.) are considered to be promising for nematode control.…”

“…There are several compounds produced by this fungus: trans-2-decenedioic nematocidal toxin that paralyzes and kills nematodes and linoleic acid, which reduces nematodes' head sizes. The mechanisms of nematodes' control are still unclear [11][12][13]. Another substance (3-octanone) produced by P. ostreatus was recently shown with the potential to kill Caenorhabditis elegans by causing the disruption of cell membrane integrity, calcium influx, etc.…”