Training protocols differently affect AMPK–PGC-1α signaling pathway and redox state in trout muscle

Pengam, Morgane; Moisan, C.; Simon, Bernard; Guernec, Anthony; Inizan, Manon; Amérand, Aline

doi:10.1016/j.cbpa.2020.110673

Cited by 8 publications

(20 citation statements)

References 63 publications

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“…Fish have separated slow and fast muscle fibres (McClelland, 2012), which are differently recruited according to the training type as in mammals (Godin et al, 2010;Jayne & Lauder, 1994). Pengam et al, 2020;Trewin et al, 2018). Other signalling pathways stimulating mitochondrial biogenesis, such as p38 mitogenactivated protein kinase and the tumour suppressor p53, are also phylogenetically conserved in fish but studied to a lesser extent than in mammals (Magnoni et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…It is known that mitochondrial biogenesis could result from the cumulative effects of transient increases in mRNA transcripts encoding mitochondrial proteins after successive exercise sessions (Perry et al, enzymatic systems is more stimulated by high exercise intensity than by moderate exercise intensity in trout muscles (Pengam et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The present study complied with principles and standards for reporting experiments in Experimental Physiology (Grundy, 2015). a Experimental groups used in a previous study (Pengam et al, 2020). HIIT, high-intensity interval training; MICT, moderate-intensity continuous training; U crit , critical swimming speed; n: number of fish per group.…”

Section: Ethical Approvalmentioning

confidence: 98%

“…efficient than a moderate intensity-high volume training at stimulating the transcripts related to the AMPK-PGC-1α signalling pathway, mitochondrial functioning and antioxidant defences (Pengam et al, 2020). These responses were also greater in red muscle (RM) than in white muscle (WM).…”

Section: New Findingsmentioning

confidence: 99%

“…Fish are a relevant model to explore mitochondrial adaptations to different modes of exercise training. Compared with mammals, fish offer the advantage of having clearly separated slow and fast fibres (McClelland, 2012; Pengam et al., 2020). This is a relevant criterion for exercise physiology studies because red oxidative and white glycolytic fibres are recruited differently depending on the type and the duration of exercise (Hvas & Oppedal, 2017).…”

Section: Introductionmentioning

confidence: 99%

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How do exercise training variables stimulate processes related to mitochondrial biogenesis in slow and fast trout muscle fibres?

Pengam¹,

Amérand²,

Simon³

et al. 2021

Experimental Physiology

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Exercise is known to be a powerful way to improve health through the stimulation of mitochondrial biogenesis in skeletal muscle, which undergoes cellular and molecular adaptations. One of the current challenges in human is to define the optimal training stimulus to improve muscle performance. Fish are relevant models for exercise training physiology studies mainly because of their distinct slow and fast muscle fibres. Using rainbow trout, we investigated the effects of six different training protocols defined by manipulating specific training variables (such as exercise intensity, volume, duration and frequency), on mRNAs and some proteins related to four subsystems (AMP-activated protein kinase-peroxisome proliferator-activated receptor γ coactivator-1α signalling pathway, mitochondrial function, antioxidant defences and lactate dehydrogenase (LDH) metabolism) in both red and white muscles (RM and WM, respectively). In both muscles, high-intensity exercise stimulated more mRNA types and enzymatic activities related to mitochondrial biogenesis than moderate-intensity exercise. For volume, duration and frequency variables, we demonstrated fibre typespecific responses. Indeed, for high-intensity interval training, RM transcript levels are increased by a low training volume, but WM transcript responses are stimulated by a high training volume. Moreover, transcripts and enzymatic activities related to mitochondria and LDH show that WM tends to develop aerobic metabolism with a high training volume. For transcript stimulation, WM requires a greater duration and frequency of exercise than RM, whereas protein adaptations are efficient with a long training duration and a high frequency in both muscles.

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